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411.
412.
Nonredundant Regulation of Rice Arbuscular Mycorrhizal Symbiosis by Two Members of the PHOSPHATE TRANSPORTER1 Gene Family 总被引:1,自引:0,他引:1
Shu-Yi Yang Mette Gr?nlund Iver Jakobsen Marianne Suter Grotemeyer Doris Rentsch Akio Miyao Hirohiko Hirochika Chellian Santhosh Kumar Venkatesan Sundaresan Nicolas Salamin Sheryl Catausan Nicolas Mattes Sigrid Heuer Uta Paszkowski 《The Plant cell》2012,24(10):4236-4251
Pi acquisition of crops via arbuscular mycorrhizal (AM) symbiosis is becoming increasingly important due to limited high-grade rock Pi reserves and a demand for environmentally sustainable agriculture. Here, we show that 70% of the overall Pi acquired by rice (Oryza sativa) is delivered via the symbiotic route. To better understand this pathway, we combined genetic, molecular, and physiological approaches to determine the specific functions of two symbiosis-specific members of the PHOSPHATE TRANSPORTER1 (PHT1) gene family from rice, ORYsa;PHT1;11 (PT11) and ORYsa;PHT1;13 (PT13). The PT11 lineage of proteins from mono- and dicotyledons is most closely related to homologs from the ancient moss, indicating an early evolutionary origin. By contrast, PT13 arose in the Poaceae, suggesting that grasses acquired a particular strategy for the acquisition of symbiotic Pi. Surprisingly, mutations in either PT11 or PT13 affected the development of the symbiosis, demonstrating that both genes are important for AM symbiosis. For symbiotic Pi uptake, however, only PT11 is necessary and sufficient. Consequently, our results demonstrate that mycorrhizal rice depends on the AM symbiosis to satisfy its Pi demands, which is mediated by a single functional Pi transporter, PT11. 相似文献
413.
K. V. Thiruvengadam 《BMJ (Clinical research ed.)》1959,2(5161):1233-1234
414.
TGF‐β gene transfer and overexpression via rAAV vectors stimulates chondrogenic events in human bone marrow aspirates 下载免费PDF全文
Janina Frisch Ana Rey‐Rico Jagadeesh Kumar Venkatesan Gertrud Schmitt Henning Madry Magali Cucchiarini 《Journal of cellular and molecular medicine》2016,20(3):430-440
Genetic modification of marrow concentrates may provide convenient approaches to enhance the chondrogenic differentiation processes and improve the repair capacities in sites of cartilage defects following administration in the lesions. Here, we provided clinically adapted recombinant adeno‐associated virus (rAAV) vectors to human bone marrow aspirates to promote the expression of the potent transforming growth factor beta (TGF‐β) as a means to regulate the biological and chondrogenic activities in the samples in vitro. Successful TGF‐β gene transfer and expression via rAAV was reached relative to control (lacZ) treatment (from 511.1 to 16.1 pg rhTGF‐β/mg total proteins after 21 days), allowing to durably enhance the levels of cell proliferation, matrix synthesis, and chondrogenic differentiation. Strikingly, in the conditions applied here, application of the candidate TGF‐β vector was also capable of reducing the hypertrophic and osteogenic differentiation processes in the aspirates, showing the potential benefits of using this particular vector to directly modify marrow concentrates to generate single‐step, effective approaches that aim at improving articular cartilage repair in vivo. 相似文献
415.
416.
Genome-scale analysis of in vivo spatiotemporal promoter activity in Caenorhabditis elegans 总被引:2,自引:0,他引:2
Dupuy D Bertin N Hidalgo CA Venkatesan K Tu D Lee D Rosenberg J Svrzikapa N Blanc A Carnec A Carvunis AR Pulak R Shingles J Reece-Hoyes J Hunt-Newbury R Viveiros R Mohler WA Tasan M Roth FP Le Peuch C Hope IA Johnsen R Moerman DG Barabási AL Baillie D Vidal M 《Nature biotechnology》2007,25(6):663-668
417.
G. Ashok Kumar Sushil K. Jalali T. Venkatesan Richard Stouthamer P. Niranjana Y. Lalitha 《Biological Control》2009,49(3):207-213
ITS-2-RFLP method was employed to distinguish 12 different trichogrammatids consisting of indigenous and exotic species such as Trichogrammatoidea armigera and Tr. bactrae, Trichogramma achaeae, T. chilonis, T. japonicum, T. embryophagum, T. pretiosum (Thelytokous Form—TF), T. brassicae, T. dendrolimi, T. evanescens and T. mwanzai. ITS-2 region was amplified; complete ITS-2 sequences of nine species were deposited in Genbank. The size of the amplified product ranged from 500 to 900 bp. Restriction enzyme digestion of ITS-2 region showed different banding profiles for these 12 species. Dichotomous keys using the size of the ITS-2 product and the restriction fragment length polymorphism for the enzymes (EcoRI, MseI, MvaI, and TaqI) allowed quick species identification of these trichogrammatids. 相似文献
418.
Saveetha Kandasamy Karthiba Loganathan Raveendran Muthuraj Saravanakumar Duraisamy Suresh Seetharaman Raguchander Thiruvengadam Balasubramanian Ponnusamy Samiyappan Ramasamy 《Proteome science》2009,7(1):1-8
Background
Heat stress (HS) and related illnesses are a major concern in military, sports, and fire brigadiers. HS results in physiologic responses of increased temperature, heart rate and sweating. In heat stroke, inflammatory response plays an important role and it is evidenced that turpentine (T) induced circulating inflammatory cytokines reduced survival rate and duration at 42°C. Here we report the alteration in the protein expression in liver cells upon HS with and without T treatment using two dimensional gel electrophoresis (2-DE), tryptic in-gel digestion and MALDI-TOF-MS/MS approaches 相似文献419.
Mutant Resources in Rice for Functional Genomics of the Grasses 总被引:2,自引:0,他引:2
420.
Selvakumar Edwardraja Rameshkumar Neelamegam Vijayaraj Ramadoss Subramanian Venkatesan Sun‐Gu Lee 《Biotechnology and bioengineering》2010,106(3):367-375
Typically, single chain Fv antibodies are unable to fold properly under a reducing cytoplasm because of the reduction of disulfide bonds. The inability to fold limits both the production of the functional scFvs and their targeting against antigens, which are generally executed in a reducing cytoplasm. In this study, the target scFv CDR was grafted with stable human consensus framework sequences, which enabled the generation of a foldable scFv in a reducing cytoplasm of Escherichia coli. Additionally, the structural features affecting the folding efficiency of the engineered scFv were identified by analyzing the predicted structure. An anti‐c‐Met scFv, which was a cytoplasmic non‐foldable protein, was redesigned as the model system. This study confirmed that the engineered anti‐c‐Met scFv was folded into its native form in the cytoplasm of E. coli BL21(DE3) without a significant loss in the specific binding activity against c‐Met antigen. The structures of the wild‐type anti‐c‐Met scFv and the engineered scFv were predicted using homology modeling. A comparative analysis based on the sequence and structure showed that the hydrophobicity of 12 solvent exposed residues decreased, and two newly formed salt bridges might have improved the folding efficiency of the engineered scFv under the reducing condition. Biotechnol. Bioeng. 2010; 106: 367–375. © 2010 Wiley Periodicals, Inc. 相似文献