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91.
Ecosystem processes in agricultural landscapes are often triggered by resource availability in crop and noncrop habitats.
We investigated how oilseed rape (OSR; Brassica napus, Brassicaceae) affects noncrop plants in managed systems and semi-natural habitat, using trophic interactions among wild
mustard (Sinapis arvensis, Brassicaceae), rape pollen beetles (Meligethes aeneus, Nitidulidae) and their parasitoids (Tersilochus heterocerus, Ichneumonidae). We exposed wild mustard as phytometer plants in two cropland habitat types (wheat field, field margin) and
three noncrop habitat types (fallow, grassland, wood margin) across eight landscapes along a gradient from simple to complex
(quantified as % arable land). Both landscape and local factors affected the abundance of rape pollen beetles and parasitoids.
Rape pollen beetle infestation and parasitism rates on these plants were lower in noncrop habitats and higher in wheat fields
and field margins, whereas beetles and parasitoids responded differently to landscape scale parameters. We found the hypothesized
spillover from OSR crop onto wild plants in surrounding habitats only for parasitoids, but not for pollen beetles. Parasitism
rates were not related to landscape simplification, but benefited from increasing proportions of OSR. In contrast, rape pollen
beetles benefited from simple landscape structures, presumably due to multi-annual population build-ups resulting from long-term
OSR planting (as part of the crop rotation). In conclusion, we showed that spillover from cropland affects parasitism rates
on related wild plants outside cropland, which has not been shown so far, but can be expected to be a widespread effect shaping
noncrop food webs. 相似文献
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93.
Mussalem JS Squaiella-Baptistão CC Teixeira D Yendo TM Thies FG Popi AF Mariano M Longo-Maugéri I 《PloS one》2012,7(3):e33955
B-1 lymphocytes are the predominant cells in mouse peritoneal cavity. They express macrophage and lymphocyte markers and are divided into B-1a, B-1b and B-1c subtypes. The role of B-1 cells is not completely clear, but they are responsible for natural IgM production and seem to play a regulatory role. An enriched B-1b cell population can be obtained from non-adherent peritoneal cell cultures, and we have previously demonstrated that these cells undergo differentiation to acquire a mononuclear phagocyte phenotype upon attachment to the substrate in vitro. Nevertheless, the B-1 cell response to antigens or adjuvants has been poorly investigated. Because killed Propionibacterium acnes exhibits immunomodulatory effects on both macrophages and B-2 lymphocytes, we analyzed whether a killed bacterial suspension or its soluble polysaccharide (PS) could modulate the absolute number of peritoneal B-1 cells in BALB/c mice, the activation status of these cells and their ability to differentiate into phagocytes in vitro. In vivo, P. acnes treatment elevated the absolute number of all B-1 subsets, whereas PS only increased B-1c. Moreover, the bacterium increased the number of B-1b cells that were positive for MHC II, TLR2, TLR4, TLR9, IL-4, IL-5 and IL-12, in addition to up-regulating TLR9, CD80 and CD86 expression. PS increased B-1b cell expression of TLR4, TLR9, CD40 and CD86, as well as IL-10 and IL-12 synthesis. Both of the treatments decreased the absolute number of B-1b cells in vitro, suggesting their early differentiation into B-1 cell-derived phagocytes (B-1CDP). We also observed a higher phagocytic activity from the phagocytes that were derived from B-1b cells after P. acnes and PS treatment. The adjuvant effect that P. acnes has on B-1 cells, mainly the B-1b subtype, reinforces the importance of B-1 cells in the innate and adaptive immune responses. 相似文献
94.
95.
Andreas O. H. Gerstner Friedrich Bootz Daniel L. Farkas Ron Martin Jörg Bendix Boris Thies 《Journal of biophotonics》2012,5(3):255-262
The aim of this study was to proof applicability of hyperspectral imaging for the analysis and classification of human mucosal surfaces in vivo. The larynx as a prototypical anatomically well‐defined surgical test area was analyzed by microlaryngoscopy with a polychromatic lightsource and a synchronous triggered monochromatic CCD‐camera. Image stacks (5 benign, 7 malignant tumors) were analyzed by established software (principal component analysis PCA, hyperspectral classification, spectral profiles). Hyperspectral image datacubes were analyzed and classified by conventional software. In PCA, images at 590–680 nm loaded most onto the first PC which typically contained 95% of the total information. Hyperspectral classification clustered the data highlighting altered mucosa. The spectral profiles clearly differed between the different groups. Hyperspectral imaging can be applied to mucosal surfaces. This approach opens the way to analyze spectral characteristics of histologically different lesions in order to build up a spectral library and to allow non‐touch optical biopsy. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
96.
Landscape perspectives on agricultural intensification and biodiversity – ecosystem service management 总被引:1,自引:0,他引:1
Teja Tscharntke Alexandra M. Klein reas Kruess Ingolf Steffan-Dewenter Carsten Thies 《Ecology letters》2005,8(8):857-874
Understanding the negative and positive effects of agricultural land use for the conservation of biodiversity, and its relation to ecosystem services, needs a landscape perspective. Agriculture can contribute to the conservation of high‐diversity systems, which may provide important ecosystem services such as pollination and biological control via complementarity and sampling effects. Land‐use management is often focused on few species and local processes, but in dynamic, agricultural landscapes, only a diversity of insurance species may guarantee resilience (the capacity to reorganize after disturbance). Interacting species experience their surrounding landscape at different spatial scales, which influences trophic interactions. Structurally complex landscapes enhance local diversity in agroecosystems, which may compensate for local high‐intensity management. Organisms with high‐dispersal abilities appear to drive these biodiversity patterns and ecosystem services, because of their recolonization ability and larger resources experienced. Agri‐environment schemes (incentives for farmers to benefit the environment) need to broaden their perspective and to take the different responses to schemes in simple (high impact) and complex (low impact) agricultural landscapes into account. In simple landscapes, local allocation of habitat is more important than in complex landscapes, which are in total at risk. However, little knowledge of the relative importance of local and landscape management for biodiversity and its relation to ecosystem services make reliable recommendations difficult. 相似文献
97.
98.
99.
Contrasting responses of arable spiders to the landscape matrix at different spatial scales 总被引:11,自引:1,他引:10
Martin H. Schmidt Carsten Thies Wolfgang Nentwig Teja Tscharntke 《Journal of Biogeography》2008,35(1):157-166
Aim Animal communities can be influenced by the composition of the surrounding landscape through immigration. Depending on habitat preferences, however, the effect of the landscape matrix can be positive or negative and can vary with scale. We tested this idea with arable spiders and tried to infer dispersal distances from relationships between local density and landscape composition at different spatial scales. Location Thirty‐eight landscapes around the cities of Göttingen and Giessen, Germany. Methods Spiders were captured with pitfall traps in one field of winter wheat in each landscape. Landscape composition around the fields was characterized at 11 scales from 95 m to 3 km radius by land‐use mapping and subsequent GIS analysis. Correlation tests were performed between landscape composition and local densities or species richness. Results In both study regions, local species richness was enhanced by non‐crop habitats on a landscape scale. The overall densities of wolf spiders (Lycosidae), long‐jawed spiders (Tetragnathidae), crab spiders (Thomisidae), and dwarf sheet spiders (Hahniidae) increased significantly in landscapes with high percentages of non‐crop habitats. Out of the 40 species tested, 19 responded positively to the percentage of non‐crop habitats in the surrounding landscape, and five responded negatively. Depending on the species, the spatial scales with the highest explanatory power ranged from 95 m to 3 km radius around the study fields, potentially reflecting dispersal distances. Main conclusions Arable spider species showed contrasting responses to the landscape context with respect both to the direction and to the spatial scale of the relationship. The variation in landscape requirements among species ensures high spider densities in a wide range of situations, which contributes to ecosystem resilience. However, species richness of arable spiders depends on heterogeneous landscapes with high percentages of non‐crop habitats. 相似文献
100.
Long-term orchard groundcover management systems affect soil microbial communities and apple replant disease severity 总被引:2,自引:0,他引:2
Apple replant disease (ARD) is a soil-disease syndrome of complex etiology that affects apple tree roots in replanted orchards,
resulting in stunted tree growth and reduced yields. To investigate whether different groundcover management systems (GMSs)
influence subsequent ARD severity, we grew apple seedlings in an outdoor nursery in pots containing orchard soil from field
plots where four GMSs had been maintained for 14 years in an orchard near Ithaca, NY, USA. The GMS treatments were: (1) pre-emergence
herbicide (Pre-H), bare soil strips maintained by applying tank-mixed glyphosate, norflurazon and diuron herbicides annually;
(2) post-emergence herbicide (Post-H), sparse weed cover maintained by applying glyphosate in May and July each year; (3)
mowed sod grass (Mowed Sod); and (4) bark mulch (Mulch). Soils were also sampled from the grass drive lane maintained between
the trees in the orchard (Grass Lane). Sampled soils (Orchard soil) were either pasteurized or left untreated, placed into
4-L pots, and planted with one apple seedling per pot. After 3 months of growth, soil (Bioassay soil) and apple tree roots
(Bioassay roots) were sampled from each pot and microbial populations colonizing samples were characterized. Seedling growth
was reduced in soils sampled from all four GMS treatments compared to the Grass Lane soils. Among the GMS treatments, seedling
biomass was greater in Pre-H than in the Post-H soil. Soil microbial communities and nutrient availability differed among
all four GMS treatments and the Grass Lane. Root-lesion (Pratylenchus sp.) nematode populations were higher in the Mowed Sod than in the other GMS treatments. Soil bacterial and fungal community
composition was assessed in Orchard and Bioassay soils and Bioassay roots with a DNA fingerprinting method (T-RFLP). Redundancy
analysis indicated that soils sampled from the different GMS treatments differentially influenced seedling biomass. A clone
library of 267 soil bacteria was developed from sampled Orchard soils and Bioassay roots. These communities were dominated
by Acidobacteria (25% of sequences), Actinobacteria (19%), δ-Proteobacteria (12%), β-Proteobacteria (10%), and these ratios
differed among the GMS soils. Members of the family Comamonadaceae were detected only in tree-row soil, not in the Grass Lanes.
The dominant sequences among 145 cloned fungi associated with apple seedling roots were Fusarium oxysporum (16% of sequences), an uncultured soil fungus submitted under DQ420986 (12%), and Rhodotorula mucilaginosa (9%). In a redundancy analysis, factors including fungal and oomycete community compositions, soil respiration rates, population
sizes of culturable bacteria and fungi, soil organic matter content, and nutrient availability, were not significant predictors
of apple seedling biomass in these soils. Different GMS treatments used by apple growers may influence subsequent ARD severity
in replanted trees, but edaphic factors commonly associated with soil fertility may not reliably predict tree-root health
and successful establishment of replanted orchards. 相似文献