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21.
Teresa Garcia Pentti Tuohimaa Jan Mester Thierry Buchou Jack-Michel Renoir Etienne-Emile Baulieu 《Biochemical and biophysical research communications》1983,113(3):960-966
Preparations of the 90K and 110K components of the chick oviduct progesterone receptor (PR) purified to near homogeneity were tested for protein kinase activity. The 90K component was shown to incorporate radioactive phosphate from [γ-32P]-ATP in the presence of Ca2+ but not of Mg2+ ions, while the 110K component was phosphorylated in the presence of Mg2+, but not of Ca2+. The enzymatic activity of the 90K polypeptide appeared selective, since added proteins (histones) did not become phosphorylated. However, all proteins present in the 110K preparations were phosphorylated in the presence of Mg2+. These data suggest that components of the chick oviduct PR display protein kinase activity. 相似文献
22.
23.
The productive gene for alpha-H chain disease protein MAL is highly modified by insertion-deletion processes 总被引:7,自引:0,他引:7
A Tsapis M Bentaboulet P Pellet E Mihaesco D Thierry M Seligmann J C Brouet 《Journal of immunology (Baltimore, Md. : 1950)》1989,143(11):3821-3827
alpha-H chain diseases (HCD) is a human lymphoproliferative disorder, characterized by the production of truncated alpha-Ig H chains, without associated L chains. In this study, we have analysed the serum protein, the alpha-HCD mRNA and the rearranged alpha-HCD gene from the leukemic cells of a patient (MAL) with alpha-HCD. The abnormal MAL serum Ig consisted of short alpha 1-chains, lacking VH and CH1 domains (only CH2 and CH3 domains were present). The alpha-HCD mRNA (1.2 kb) was shorter than a normal alpha-mRNA (2 kb); the corresponding cDNA had sequences for the leader, a 84-bp sequence of unknown origin and the CH2 and CH3 exons. The establishment of the sequence of the productive alpha-HCD MAL allele revealed two major deletions; that of the VH region as well as that of the CH1 region. The JH region is altered by multiple mutations, small insertions and a duplication of the psi JH3 region. A large insert (INS1), of 360 bp (containing the 84 bp exon found in the cDNA), replaces the deleted VH region. INS1 is non-Ig related and apparently of nongenomic origin. A large second insert (509 bp), is located between the enhancer and the switch region. Insert2 contains repetitive non-Ig-related sequences and a small Ig-related sequence. All these alterations resulted in an abnormal mRNA, which comprises the leader, a 84-bp alien exon derived from INS1 and the CH2 and CH3 exons of the alpha 1-gene. 相似文献
24.
Thierry Becquer Denis Merlet Jean-Pierre Boudot James Rouiller Francis Gras 《Plant and Soil》1990,125(1):95-107
Nitrate uptake and leaching were measured during one year in a declined fir forest on the Vosges highlands (eastern France),
in order to investigate whether excess nitrification could be responsible for a deleterious acidification of the ecosystem.
Nitrate uptake by the vegetation was active mainly from spring to early fall, and then reached about 66 kg N ha-1. No significant leaching loss occurred during the growth period of the vegetation. Significant nitrate leaching occurred
in winter (about 17 kg N ha-1). During fall and winter the nitrification rate was of the same magnitude as values reported for other ecosystems, and, thus,
was not considered to be abnormaly strong. No abnormal temporal discoupling of nitrate production and nitrate uptake occurred
in the ecosystem, and forest decline must therefore have some other cause. 相似文献
25.
26.
Fatal attack on an infant by an adult female tonkean macaque 总被引:1,自引:0,他引:1
We describe a fatal attack on a 10-month-old female infant by an adult female Tonkean macaque (Macaca tonkeana)in a semi-free-ranging group. During an intense conflict involving many group members, an adult female, the second most dominant
in the group, attacked the infant of a lower-ranking female. The attacker remained near the victim for approximately 20 min,
grabbing and biting it repeatedly, until the mother retrieved the infant. She died 2 days later. An autopsy revealed no lethal
injury;the main cause of death was starvation or dehydration or both. We discuss social conditions under which a fatal attack by
an adult female might occur. 相似文献
27.
Identification and expression of water stress- and abscisic acid-regulated genes in a drought-tolerant sunflower genotype 总被引:17,自引:0,他引:17
28.
A new gene coding for an antigen recognized by autologous cytolytic T lymphocytes on a human renal carcinoma 总被引:7,自引:0,他引:7
Béatrice Gaugler Nathalie Brouwenstijn Valérie Vantomme Jean-Pierre Szikora Corry W. Van der Spek Jean-Jacques Patard Thierry Boon Peter Schrier Benoît J. Van den Eynde 《Immunogenetics》1996,44(5):323-330
Previous reports have described antigens that are recognized on human melanoma cells by autologous cytolytic T lymphocytes
(CTL). The genes coding for a number of these antigens have been identified. Here we report the cloning of a gene that codes
for an antigen recognized by autologous CTL on a human renal carcinoma cell line. This antigen is presented byHLA-B7 and is encoded by a new gene that we have namedRAGE1. No expression ofRAGE1 was found in normal tissues other than retina. RAGE1 expression was found in only one of 57 renal cell carcinoma samples,
and also in some sarcomas, infiltrating bladder carcinomas, and melanomas. This represents the first identification of an
antigen recognized by autologous CTL on a renal tumor. 相似文献
29.
Crystal structure of a prokaryotic aspartyl tRNA-synthetase. 总被引:3,自引:3,他引:0
M Delarue A Poterszman S Nikonov M Garber D Moras J C Thierry 《The EMBO journal》1994,13(14):3219-3229
The crystal structure of Thermus thermophilus aspartyl tRNA-synthetase (AspRS) refined at 2.5 A resolution is described. This molecular structure is a textbook illustration of the modular organization of aminoacyl-tRNA synthetases. In addition to the three domains found in yeast AspRS, each monomer exhibits a module specific to prokaryotic enzymes, which corresponds to a helix-turn-helix motif in yeast AspRS, a domain implicated in the stabilization of the complex with tRNA. Its topology matches that of the histidine-containing phosphocarrier HPr which has been linked recently to another group of proteins containing the ferredoxin fold. We propose a more extensive alignment of these folds, which involves a circular permutation of the sequences and changes the point of entry of the whole domain. The C-terminal extension, another prokaryotic characteristic, leads to a significant increase in the network of interaction at the dimer interface. Some potential communication pathways suggest how a transfer of information between the two active sites of the homodimer might occur. Most of the residues involved belong to the class II-specific motifs in correlation with the dimeric state of nearly all class II enzymes. The T. thermophilus enzyme exhibits some features not found in any of the six other known AspRSs from mesophilic organisms. 相似文献
30.
Bienvenu Thierry Hubert Dominique Fonknechten Nuria Dusser Daniel Kaplan Jean Claude Beldjord Cherif 《Human genetics》1994,94(1):65-68
Human Genetics - Cystic fibrosis is caused by mutations in the cystic fibrosis transmembrane conductance regulator gene (CFTR). Analysis of DNA from a pancreatic sufficient patient by means of... 相似文献