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51.
Fabrice Collin Joëlle Hindo Patrice Thérond Martine Couturier Claudine Cosson Daniel Jore Monique Gardès-Albert 《Biochimie》2010
An investigation of radiation-induced oxidation of aqueous bovine serum albumin (BSA) in the presence of linoleate (LH) at pH 10.5 has been carried out in order to better understand the respective oxidative processes involved in both lipid and protein phases. Solutions containing BSA (15 μmol L−1) and linoleate (15–600 μmol L−1) below the critical micellar concentration (cmc = 2000 μmol L−1), have been irradiated by γ-rays (137Cs) at radiation doses ranging from 10 to 400 Gy (dose rate 9.5 Gy min−1). It can be noticed that, in the absence of BSA, the main hydroperoxides formed from HO•-induced linoleate oxidation below the cmc, do not exhibit a conjugated dienic structure. This was also verified in the presence of BSA. Selected chemical markers of oxidation have been monitored: non-conjugated dienic hydroperoxides and conjugated dienes (without hydroperoxide function) for linoleate oxidation, and carbonyl groups for BSA oxidation. We have shown that for the lowest linoleate concentration (15 μmol L−1) in the presence of BSA (15 μmol L−1), the formation of conjugated dienes was not observed, meaning that LH was not exposed to HO• radicals attack. However, non-conjugated dienic lipid hydroperoxides were simultaneously detected, indicating that LH was secondarily oxidised by BSA oxidised species. Moreover, the oxidation of linoleate was found to be enhanced by the presence of BSA. For the highest linoleate concentration (600 μmol L−1), the expected protection of BSA by LH was not observed, even if LH monomers were responsible for the total scavenging of HO• radicals. In this latter case, the formation of non-conjugated dienic lipid hydroperoxides was lower than expected. Those results showed that BSA was not oxidised by the direct action of HO• radicals but was undergoing a secondary oxidation by non-dienic lipid hydroperoxides and/or lipid radical intermediates, coming from the HO•-induced linoleate oxidation. 相似文献
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Kazuyuki Tanabe Toshihiro Mita Thibaut Jombart Anders Eriksson Shun Horibe Nirianne Palacpac Lisa Ranford-Cartwright Hiromi Sawai Naoko Sakihama Hiroshi Ohmae Masatoshi Nakamura Marcelo U. Ferreira Ananias A. Escalante Franck Prugnolle Anders Björkman Anna Färnert Akira Kaneko Toshihiro Horii Andrea Manica Hirohisa Kishino Francois Balloux 《Current biology : CB》2010,20(14):1283-1289
54.
M. Gust E. Vulliet B. Giroud F. Garnier S. Couturier J. Garric T. Buronfosse 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2010,878(19):1487-1492
The role of vertebrate-like sex-steroids (testosterone, T, progesterone, P, and 17β-estradiol, E2) in molluscs is still debated, but they could represent potential biomarkers of endocrine disruption. A radioimmunoassay (RIA) and a liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS) methods have been developed and compared to measure their levels in a gastropod snail Potamopyrgus antipodarum. Both methods showed a good reproducibility despite the complex matrix and the very low levels of vertebrate-like sex-steroids. Only T and P were detected using the LC–MS/MS method, while the RIA method reached lower detection limits and enabled the detection of all three steroids. Results indicated that T and P were mainly present as unconjugated forms. Both methods were compared in the analysis of snails exposed to waste water treatment plant effluents and led to the same conclusions concerning the modulation of steroids levels. Moreover, they both were in agreement concerning T measurements. On the other hand, LC–MS/MS appeared to be more suitable when analyzing P levels due to a low sensitivity of the RIA method. As E2 was not measured using the LC–MS/MS method because of a higher detection limit compared to the other steroids, the results obtained with the RIA method should be interpreted with caution. LC–MS/MS remains the gold standard for sex-steroid determinations, however a relevant and alternative method based on RIA was developed, requiring fewer organisms. RIA seems a promising method as a screening tool for experimental use, allowing comparison of sex-steroid levels in the mudsnail both in laboratory and in field experiments. 相似文献
55.
Mariana Royer Gaëtan Herbette Véronique Eparvier Jacques Beauchêne Bernard Thibaut Didier Stien 《Phytochemistry》2010,71(14-15):1708-1713
In order to explain the durability of the Moraceae plant family, phytochemistry of Bagassa guianensis was performed. Ethyl acetate extract was obtained from the heartwood and 18 secondary metabolites were isolated, including 6 moracins [6-O-methyl-moracin M, 6-O-methyl-moracin N and moracin Z; previously identified: moracin M, moracin N and moracin P], 8 stilbenoids [presently identified: (?)-epialboctalol and arachidin 4; previously identified: alboctalol, trans-resveratrol, arachidin 2, trans-oxyresveratrol and artogomezianol], 3 previously identified flavonoids, steppogenin, katuranin and dihydromorin, β-sitosterol and resorcinol. Previous studies suggest that stilbenoids are responsible for the natural durability of wood. Our study has determined that B. guianensis is closely related to Morus sp. in phylogeny and should be included in the Moreae sensu stricto tribe of the Moraceae family. 相似文献
56.
David Navarro Marie Couturier Gabriela Ghizzi Damasceno da Silva Jean-Guy Berrin Xavier Rouau Marcel Asther Christophe Bignon 《Microbial cell factories》2010,9(1):58
Background
To reduce the production cost of bioethanol obtained from fermentation of the sugars provided by degradation of lignocellulosic biomass (i.e., second generation bioethanol), it is necessary to screen for new enzymes endowed with more efficient biomass degrading properties. This demands the set-up of high-throughput screening methods. Several methods have been devised all using microplates in the industrial SBS format. Although this size reduction and standardization has greatly improved the screening process, the published methods comprise one or more manual steps that seriously decrease throughput. Therefore, we worked to devise a screening method devoid of any manual steps. 相似文献57.
González-Fernández Andrea Segarra Joel Sunny Armando Couturier Stephane 《Biodiversity and Conservation》2022,31(3):871-894
Biodiversity and Conservation - In 2013, the protection status of the Nevado de Toluca Volcano was changed from National Park to a less restrictive category. Much controversy has arisen surrounding... 相似文献
58.
Howard Donninger Jennifer Clark Francesca Rinaldo Nicholas Nelson Thibaut Barnoud M. Lee Schmidt Katharine R. Hobbing Michele D. Vos Brian Sils Geoffrey J. Clark 《Molecular and cellular biology》2015,35(1):277-287
RASSF1A may be the most frequently inactivated tumor suppressor identified in human cancer so far. It is a proapoptotic Ras effector and plays an important role in the apoptotic DNA damage response (DDR). We now show that in addition to DDR regulation, RASSF1A also plays a key role in the DNA repair process itself. We show that RASSF1A forms a DNA damage-regulated complex with the key DNA repair protein xeroderma pigmentosum A (XPA). XPA requires RASSF1A to exert full repair activity, and RASSF1A-deficient cells exhibit an impaired ability to repair DNA. Moreover, a cancer-associated RASSF1A single-nucleotide polymorphism (SNP) variant exhibits differential XPA binding and inhibits DNA repair. The interaction of XPA with other components of the repair complex, such as replication protein A (RPA), is controlled in part by a dynamic acetylation/deacetylation cycle. We found that RASSF1A and its SNP variant differentially regulate XPA protein acetylation, and the SNP variant hyperstabilizes the XPA-RPA70 complex. Thus, we identify two novel functions for RASSF1A in the control of DNA repair and protein acetylation. As RASSF1A modulates both apoptotic DDR and DNA repair, it may play an important and unanticipated role in coordinating the balance between repair and death after DNA damage. 相似文献
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60.
Gilbert Richarme Mouadh Mihoub Julien Dairou Linh Chi Bui Thibaut Leger Aazdine Lamouri 《The Journal of biological chemistry》2015,290(3):1885-1897
Glycation is an inevitable nonenzymatic covalent reaction between proteins and endogenous reducing sugars or dicarbonyls (methylglyoxal, glyoxal) that results in protein inactivation. DJ-1 was reported to be a multifunctional oxidative stress response protein with poorly defined function. Here, we show that human DJ-1 is a protein deglycase that repairs methylglyoxal- and glyoxal-glycated amino acids and proteins by acting on early glycation intermediates and releases repaired proteins and lactate or glycolate, respectively. DJ-1 deglycates cysteines, arginines, and lysines (the three major glycated amino acids) of serum albumin, glyceraldehyde-3-phosphate dehydrogenase, aldolase, and aspartate aminotransferase and thus reactivates these proteins. DJ-1 prevented protein glycation in an Escherichia coli mutant deficient in the DJ-1 homolog YajL and restored cell viability in glucose-containing media. These results suggest that DJ-1-associated Parkinsonism results from excessive protein glycation and establishes DJ-1 as a major anti-glycation and anti-aging protein. 相似文献