全文获取类型
收费全文 | 344篇 |
免费 | 16篇 |
专业分类
360篇 |
出版年
2018年 | 4篇 |
2017年 | 4篇 |
2015年 | 4篇 |
2014年 | 5篇 |
2013年 | 14篇 |
2012年 | 12篇 |
2011年 | 13篇 |
2010年 | 4篇 |
2009年 | 5篇 |
2008年 | 9篇 |
2007年 | 7篇 |
2006年 | 12篇 |
2005年 | 8篇 |
2004年 | 7篇 |
2003年 | 10篇 |
2002年 | 8篇 |
1996年 | 4篇 |
1994年 | 6篇 |
1993年 | 4篇 |
1992年 | 4篇 |
1991年 | 8篇 |
1988年 | 4篇 |
1986年 | 5篇 |
1985年 | 4篇 |
1982年 | 3篇 |
1981年 | 8篇 |
1980年 | 6篇 |
1979年 | 8篇 |
1973年 | 4篇 |
1972年 | 4篇 |
1971年 | 4篇 |
1967年 | 3篇 |
1966年 | 3篇 |
1965年 | 5篇 |
1961年 | 3篇 |
1958年 | 4篇 |
1957年 | 5篇 |
1955年 | 3篇 |
1952年 | 4篇 |
1939年 | 4篇 |
1937年 | 4篇 |
1936年 | 4篇 |
1932年 | 3篇 |
1927年 | 3篇 |
1925年 | 3篇 |
1924年 | 3篇 |
1914年 | 3篇 |
1863年 | 3篇 |
1861年 | 3篇 |
1860年 | 4篇 |
排序方式: 共有360条查询结果,搜索用时 15 毫秒
81.
Dr. Franz Theodor Münzer 《Development genes and evolution》1923,98(1-2):249-282
Ohne Zusammenfassung 相似文献
82.
Theodor Dürrigl 《International journal of biometeorology》1965,9(2):199
Ohne Zusammenfassung 相似文献
83.
84.
85.
86.
Ureteric peristalsis, which occurs via alternating contraction and relaxation of ureteric smooth muscle, ensures the unidirectional flow of urine from the kidney to the bladder. Understanding of the molecular mechanisms underlying ureteric excitation–contraction coupling, however, is limited. To address these knowledge deficits, and in particular to test the hypothesis that Ca2+ sensitization via activation of the RhoA/Rho-associated kinase (ROK) pathway plays an important role in ureteric smooth muscle contraction, we carried out a thorough characterization of the electrical activity, Ca2+ signaling, MYPT1 (myosin targeting subunit of myosin light chain phosphatase, MLCP) and myosin regulatory light chain (LC20) phosphorylation, and force responses to membrane depolarization induced by KCl (electromechanical coupling) and carbachol (CCh) (pharmacomechanical coupling). The effects of ROK inhibition on these parameters were investigated. We conclude that the tonic, but not the phasic component of KCl- or CCh-induced ureteric smooth muscle contraction is highly dependent on ROK-catalyzed phosphorylation of MYPT1 at T855, leading to inhibition of MLCP and increased LC20 phosphorylation. 相似文献
87.
Dimitrios Kalpaxis Ilse Zündorf Herbert Werner Norbert Reindl Emmanuelle Boy-Marcotte Michel Jacquet Theodor Dingermann 《Molecular & general genetics : MGG》1991,225(3):492-500
Summary In the cellular slime mould Dictyostelium discoideum the two enzymatic activities of the pyrimidine pathway, orotidine-5-phosphate decarboxylase (EC 4.1.1.23; OMPdecase) and orotate phosphoribosyl transferase (EC 2.4.2.10; OPRTase), are encoded by a single gene (DdPYR5-6). As in higher eukaryotes the bifunctional enzyme is referred to as UMP synthase. Here we present a method that allows efficient generation and selection of mutants lacking UMP synthase. D. discoideum cells are transformed with either of two different types of plasmids. One plasmid type contains no sequences homologous to the UMP synthase gene whereas the other type contains at least parts of this gene. UMP synthase– mutants, which were positively selected for in the presence of 5-fluoroorotic acid (5-FOA), were obtained with both plasmids. However, mutation rates were at least one order of magnitude higher if plasmids containing various portions of the UMP synthase gene were used as opposed to plasmids that lack any homology to the UMP synthase locus. Several mutant strains were extensively characterized. These strains lack OMPdecase activity and exhibit in addition to 5-FOA resistance a ura
– phenotype. All mutants carry UMP synthase loci with deletions of various extents but integration of transforming plasmids was not detected. This efficient generation of 5-FOA resistance is part of a proposed complex selection scheme which allows multiple rounds of transformation of D. discoideum. 相似文献
88.
89.
90.