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41.
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Six strains of branched blue-green algae were examined in a search for lines to an explanation for apparent noncompetitiveness. Classical taxonomic treatments were often inadequate for confident species determination; forms that were very differ their morphologically frequently “keyed” to the same species. Strain distinctions based on comparative morphological and physiological studies were inconclusive. With the exception of 1 species, there were no substantial differences in physiological rates. In addition to the branching habit, diversity of cellular form was found to be an outstanding group characteristic. Individual cells of developing trichomes became a spore-like and then divided to produce either cell clusters or lateral branches. The eventual separation of daughter cells by sheath materials may be analogous to spore formation in Nostocales and could be the factor which determines the nature of these organisms. The Stigonemataceae may not represent the pinnacle of blue-green algal development but may, instead, be a primitive and basic form linking coccoid and filamentous algae. 相似文献
43.
Hydrobiologia - In regulated rivers, dams alter longitudinal gradients in flow regimes, geomorphology, water quality and temperature with associated impacts on aquatic biota. Unregulated... 相似文献
44.
Eugen F. Mesaros Jason P. BurkeJonathan D. Parrish Benjamin J. DuganAndrew V. Anzalone Thelma S. AngelesMark S. Albom Lisa D. AimoneMatthew R. Quail Weihua WanLihui Lu Zeqi HuangMark A. Ator Bruce A. RuggeriMangeng Cheng Gregory R. Ott Bruce D. Dorsey 《Bioorganic & medicinal chemistry letters》2011,21(6):1900
45.
Nina Bertaux-Skeirik Rui Feng Michael A. Schumacher Jing Li Maxime M. Mahe Amy C. Engevik Jose E. Javier Richard M. Peek Jr Karen Ottemann Veronique Orian-Rousseau Gregory P. Boivin Michael A. Helmrath Yana Zavros 《PLoS pathogens》2015,11(2)
The cytotoxin-associated gene (Cag) pathogenicity island is a strain-specific
constituent of Helicobacter pylori (H. pylori) that
augments cancer risk. CagA translocates into the cytoplasm where it stimulates cell
signaling through the interaction with tyrosine kinase c-Met receptor, leading
cellular proliferation. Identified as a potential gastric stem cell marker,
cluster-of-differentiation (CD) CD44 also acts as a co-receptor for c-Met, but
whether it plays a functional role in H. pylori-induced epithelial
proliferation is unknown. We tested the hypothesis that CD44 plays a functional role
in H. pylori-induced epithelial cell proliferation. To assay changes
in gastric epithelial cell proliferation in relation to the direct interaction with
H. pylori, human- and mouse-derived gastric organoids were
infected with the G27 H. pylori strain or a mutant G27 strain
bearing cagA deletion (∆CagA::cat). Epithelial proliferation
was quantified by EdU immunostaining. Phosphorylation of c-Met was analyzed by
immunoprecipitation followed by Western blot analysis for expression of CD44 and
CagA. H. pylori infection of both mouse- and human-derived gastric
organoids induced epithelial proliferation that correlated with c-Met
phosphorylation. CagA and CD44 co-immunoprecipitated with phosphorylated c-Met. The
formation of this complex did not occur in organoids infected with
∆CagA::cat. Epithelial proliferation in response to
H. pylori infection was lost in infected organoids derived from
CD44-deficient mouse stomachs. Human-derived fundic gastric organoids exhibited an
induction in proliferation when infected with H. pylorithat was not
seen in organoids pre-treated with a peptide inhibitor specific to CD44. In the
well-established Mongolian gerbil model of gastric cancer, animals treated with CD44
peptide inhibitor Pep1, resulted in the inhibition of H.
pylori-induced proliferation and associated atrophic gastritis. The current
study reports a unique approach to study H. pylori interaction with
the human gastric epithelium. Here, we show that CD44 plays a functional role in
H. pylori-induced epithelial cell proliferation. 相似文献
46.
Anahid H. Saradjian Dany Paleressompoulle Didier Louber Thelma Coyle Jean Blouin Laurence Mouchnino 《PloS one》2014,9(9)
We recently found that the cortical response to proprioceptive stimulation was greater when participants were planning a step than when they stood still, and that this sensory facilitation was suppressed in microgravity. The aim of the present study was to test whether the absence of gravity-related sensory afferents during movement planning in microgravity prevented the proprioceptive cortical processing to be enhanced. We reestablished a reference frame in microgravity by providing and translating a horizontal support on which the participants were standing and verified whether this procedure restored the proprioceptive facilitation. The slight translation of the base of support (lateral direction), which occurred prior to step initiation, stimulated at least cutaneous and vestibular receptors. The sensitivity to proprioceptive stimulation was assessed by measuring the amplitude of the cortical somatosensory-evoked potential (SEP, over the Cz electrode) following the vibration of the leg muscle. The vibration lasted 1 s and the participants were asked to either initiate a step at the vibration offset or to remain still. We found that the early SEP (90–160 ms) was smaller when the platform was translated than when it remained stationary, revealing the existence of an interference phenomenon (i.e., when proprioceptive stimulation is preceded by the stimulation of different sensory modalities evoked by the platform translation). By contrast, the late SEP (550 ms post proprioceptive stimulation onset) was greater when the translation preceded the vibration compared to a condition without pre-stimulation (i.e., no translation). This suggests that restoring a body reference system which is impaired in microgravity allowed a greater proprioceptive cortical processing. Importantly, however, the late SEP was similarly increased when participants either produced a step or remained still. We propose that the absence of step-induced facilitation of proprioceptive cortical processing results from a decreased weight of proprioception in the absence of balance constraints in microgravity. 相似文献
47.
48.
The role of CagA status in gastric and extragastric complications of Helicobacter pylori. 总被引:11,自引:0,他引:11
G I Perez-Perez R M Peek A J Legath P R Heine L B Graff 《Journal of physiology and pharmacology》1999,50(5):833-845
Two major markers of virulence have been described in H. pylori. The first is a secreted protein (VacA) that is toxic to human cells in tissue culture. This cytotoxin causes vacuolation of epithelial cells in vitro and induces epithelial cell damage in mice. The second is a 40-Kb pathogenicity island for which the gene cagA (cytotoxin-associated gene A) is a marker. Approximately 60% of H. pylori isolates in Western countries are cagA+. The protein encoded by cagA+ has a molecular weight of 120-140 kDa and exhibits sequence heterogeneity among strains isolated from Western and Eastern countries. Although no specific function has been identified for CagA, there is increasing evidence that cagA+ strains are associated with increased intensity of gastric inflammation and increased mucosal concentration of particular cytokines including interleukin 8. Inactivation of picB (Hp 0544) or any of several other genes in the cag island ablates the enhanced IL-8 secretion of human gastric epithelial cells in tissue culture. Furthermore, persons colonized with cagA+ strains have an increased risk of developing more severe gastric diseases such as peptic ulcer and distal (non-cardia) gastric cancer than those harboring cagA- strains. We investigated the role of cagA status in both gastroduodenal and extragastroduodenal disease with H. pylori. Among the diseases limited to the antrum and body of the stomach and the duodenum, we demonstrated a correlation between CagA seropositivity and peptic ulcer disease. We also showed correlation between distal gastric cancer rated and CagA prevalence in populations in both developed and developing countries. In addition, we found that for several Asian populations, the relationship between CagA seropositivity and gastroduodenal diseases was complex. For extragastroduodenal diseases, our results confirmed previous reports that demonstrated that CagA status did not play a role in diseases such as rheumatoid arthritis and hyperemesis gravidarum. However, we found a clear negative association between the presence of a positive response to CagA and esophageal diseases. Therefore, CagA seropositivity (and thus gastric carriage) is associated with increased risks of certain diseases (involving the lower stomach and duodenum) and decreased risks of GERD and its sequelae. This apparent paradox can best be explained by differences in the interaction of cagA+ and cagA- strains with their hosts. 相似文献
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