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81.
Melo TG Almeida DS de Meirelles Mde N Pereira MC 《European journal of cell biology》2004,83(10):531-540
Chagas' disease cardiomyopathy is an important manifestation of Trypanosoma cruzi infection, leading to cardiac dysfunction and serious arrhythmias. We have here investigated by indirect immunofluorescence assay the distribution of vinculin, a focal adhesion protein with a major role in the transmission of contraction force, during the T. cruzi-cardiomyocyte infection in vitro and in vivo. No change in vinculin distribution was observed after 24 h of infection, where control and T. cruzi-infected cardiomyocytes displayed vinculin localized at costameres and intercalated discs. On the other hand, a clear disruption of vinculin costameric distribution was noted after 72 h of infection. A significant reduction in the levels of vinculin expression was observed at all times of infection. In murine experimental Chagas' disease, alteration in the vinculin distribution was also detected in the infected myocardium, with no costameric staining in infected myocytes and irregular alignment of intercalated discs in cardiac fibers. These data suggest that the disruption of costameric vinculin distribution and the enlargement of interstitial space due to inflammatory infiltration may contribute to the reduction of transmission of cardiac contraction force, leading to alterations in the heart function in Chagas' disease. 相似文献
82.
Maria de Nazaré C. Soeiro Fernando Costa e Silva Filho Maria de Nazareth Leal de Meirelles 《Cell biochemistry and biophysics》1995,26(1):21-44
The surface charge of heart muscle cells (HMC) andTrypanosoma cruzi trypomastigotes was estimated during their interaction by means of zeta potential (ZP). Metacyclic and bloodstream trypomastigote, but not amastigote forms, are able to decrease the surface charge of HMC as well as other nonphagocytic cells. However, no alteration could be detected onT. cruzi-infected macrophage cell line. Trypomastigote forms collected from the supernatant after 20 h of contact with HMC also have their ZP value decreased. The analysis of the surface components of both the parasite and HMC involved in such interaction was also carried out. Assays concerning the kinetics of the cell-parasite interaction demonstrated the influence of parasite surface anionogenicity during its interaction with HMC. The binding of bloodstream forms to HMC was enhanced after their incubation with cationized ferritin (CF), whereas phospholipase C and neuraminidase treatments improved and trypsin treatment inhibited parasite uptake in HMC. Conversely, the incubation of HMC with phospholipase C impaired, and with trypsin enhanced, the interiorization of the parasites. These results suggest that trypomastigote forms ofT. cruzi may process the surface of HMC and its own surface either by removing molecules or by exposing ligands for their internalization. 相似文献
83.
T C Araújo Jorge H S Barbosa A L Moreira W De Souza M N Meirelles 《Zeitschrift für Parasitenkunde (Berlin, Germany)》1986,72(5):577-584
The process of interaction of bloodstream trypomastigotes from the myotropic CL and Colombiana strains and the macrophagotropic Y strain of Trypanosoma cruzi with mouse myoblasts and myotubes was analysed. After 24 h of parasite-host cell interaction, parasites from the CL and Colombiana strains appeared to be more infective to myoblasts than those from the Y strain. Parasites from the Colombiana strain were more infective for myotubes than those from the Y strain, while those from the CL strain showed very a low ability to infect the cells. For all strains the infectivity was low for short periods of interaction, increasing with time. Myoblasts infected with parasites from the Y strain fused with other infected and uninfected cells to form myotubes. However, the process of fusion was blocked when the myoblasts were infected with parasites from the CL and Colombiana strains. These data indicate a different behavior of muscle cells when in contact with myotropic or non-myotropic strains of T. cruzi. 相似文献
84.
Marcos E. Buzanskas Daniela A. Grossi Ricardo V. Ventura Flávio S. Schenkel Mehdi Sargolzaei Sarah L. C. Meirelles Fabiana B. Mokry Roberto H. Higa Maurício A. Mudadu Marcos V. G. Barbosa. da Silva Simone C. M. Niciura Roberto A. A. Torres. Júnior Maurício M. Alencar Luciana C. A. Regitano Danísio P. Munari 《PloS one》2014,9(4)
Studies are being conducted on the applicability of genomic data to improve the accuracy of the selection process in livestock, and genome-wide association studies (GWAS) provide valuable information to enhance the understanding on the genetics of complex traits. The aim of this study was to identify genomic regions and genes that play roles in birth weight (BW), weaning weight adjusted for 210 days of age (WW), and long-yearling weight adjusted for 420 days of age (LYW) in Canchim cattle. GWAS were performed by means of the Generalized Quasi-Likelihood Score (GQLS) method using genotypes from the BovineHD BeadChip and estimated breeding values for BW, WW, and LYW. Data consisted of 285 animals from the Canchim breed and 114 from the MA genetic group (derived from crossings between Charolais sires and ½ Canchim + ½ Zebu dams). After applying a false discovery rate correction at a 10% significance level, a total of 4, 12, and 10 SNPs were significantly associated with BW, WW, and LYW, respectively. These SNPs were surveyed to their corresponding genes or to surrounding genes within a distance of 250 kb. The genes DPP6 (dipeptidyl-peptidase 6) and CLEC3B (C-type lectin domain family 3 member B) were highlighted, considering its functions on the development of the brain and skeletal system, respectively. The GQLS method identified regions on chromosome associated with birth weight, weaning weight, and long-yearling weight in Canchim and MA animals. New candidate regions for body weight traits were detected and some of them have interesting biological functions, of which most have not been previously reported. The observation of QTL reports for body weight traits, covering areas surrounding the genes (SNPs) herein identified provides more evidence for these associations. Future studies targeting these areas could provide further knowledge to uncover the genetic architecture underlying growth traits in Canchim cattle. 相似文献
85.
Juliana Saraiva Claudia Meirelles Siqueira Carlos Henrique Tomich de Paula da Silva Vinicius Barreto da Silva Valeria Gomes Tudella Rosângela Silva 《Journal of enzyme inhibition and medicinal chemistry》2013,28(2):599-606
The effects on mitochondrial respiration and complex I NADH oxidase activity of cubebin and derivatives were evaluated. The compounds inhibited the state 3 glutamate/malate-supported respiration of hamster liver mitochondria with IC50 values ranging from 12.16 to 83.96 μM. NADH oxidase reaction was evaluated in submitochondrial particles. The compounds also inhibited this activity, showing the same order of potency observed for effects on state 3 respiration, as well as a tendency towards a non-competitive type of inhibition (KI values ranging from 0.62 to 16.1 μM). A potential binding mode of these compounds with complex I subunit B8, assessed by docking calculations, is proposed. 相似文献
86.
Antioxidant responses and deregulation of epigenetic writers and erasers link oxidative stress and DNA methylation in bovine blastocysts 下载免费PDF全文
87.
Danuta Sastre Nathália Nogueira da Costa André Luiz Alves de Sá Stefanne Dhúllia Braga Conceição Marcos Roberto Chiaratti Paulo Roberto Adona Samuel Guemra Flávio Vieira Meirelles Simone do Socorro Damasceno Santos Leonardo Sena Otávio Mitio Ohashi Eduardo José Melo dos Santos Moysés dos Santos Miranda 《Theriogenology》2014
88.
Fernanda Menezes de Oliveira e Silva Vitor Luz Carvalho Juliana Plácido Guimarães Jociery Einhardt Vergara-Parente Ana Carolina Oliveira de Meirelles Miriam Marmontel Maria Angelica Miglino 《Zoomorphology》2014,133(3):343-350
The objective was to determine the prevalence of accessory spleens in cetaceans stranded on the north and northeastern coasts of Brazil and to describe their macroscopic and microscopic characteristics, thereby providing insights into the contribution of these structures to the immune system of cetaceans. Sixty-three Odontocetes and Mysticetes (total of 14 species), male and female, ranging from calves to adults, stranded from 2009 to 2013 on the Brazilian north and northeastern coasts, were evaluated. Accessory spleens were present in 38 animals (60.3 %), with 1–14 accessory spleens per animal. Their location varied among species, ranging from firmly adherent to the spleen, to the large curvature of the first stomach or both. The presence of these structures was apparently not related to age or sex. However, there was a higher prevalence in animals with a greater body size and known to make deeper dives. Both primary and accessory spleens had similar macroscopic morphology with no demarcation between cortex and medulla. Both primary and accessory spleens had similar histological characteristics. Furthermore, it was noteworthy that germinal centers became more discrete and reduced in number with increasing age. In conclusion, we inferred that accessory spleens may be an additional mechanism for adaptation to diving and that they have a complementary reservoir function and thus can be considered compensatory lymphoid organs to splenic activity. 相似文献
89.
Vânia Regina Pivello Imma Oliveras Heloísa Sinátora Miranda Mundayatan Haridasan Margarete Naomi Sato Sérgio Tadeu Meirelles 《Plant and Soil》2010,337(1-2):111-123
Fire is common in savannas but its effects on soil are poorly understood. We analyzed long-term effects of fire on surface soil of an open Brazilian savanna (campo sujo) in plots submitted to different fire regimes during 18 years. The five fire regimes were: unburned, quadrennial fires in middle dry season, and biennial fires in early, middle or late dry season. Soil was collected during the wet and the middle dry season of 2008, and analyzed for pH, organic matter, total N, potential acidity, exchangeable cations and available P, S, Mn, Cu, Zn and Fe. We applied multivariate analysis to search for patterns related to fire regimes, and to local climate, fuel, and fire behavior. Spearman test was used to establish correlations between soil variables and the multivariate analysis gradient structure. Seasonal differences were tested using t-test. We found evidence of long-term fire effects: the unburned plot was segregated mainly by lower soil pH; the quadrennial plot was also segregated by lower soil pH and higher amount of exchangeable cations; the time of burning during the dry season in biennial plots did not significantly affect soil availability of nutrients. Differences in elements amounts due to the season of soil sampling (wet or dry) were higher than due to the effect of fires. Higher availability of nutrients in the soil during the wet season was probably related to higher nutrient inputs via rainfall and higher microbial activity. 相似文献
90.
Tiana Baqueiro Momtchilo Russo Virgínia MG Silva Thayna Meirelles Pablo RS Oliveira Eliane Gomes Renato Barboza Ana T Cerqueira-Lima Camila A Figueiredo Lain Pontes-de-Carvalho Neuza M Alcantara-Neves 《Respiratory research》2010,11(1):51