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排序方式: 共有432条查询结果,搜索用时 15 毫秒
81.
82.
H Hondermarck J Sy R A Bradshaw S M Arfin 《Biochemical and biophysical research communications》1992,189(1):280-288
Dipeptide inhibitors of the ubiquitin-dependent proteolysis pathway governed by N-terminal recognition (N-end rule) in reticulocyte lysates significantly suppress NGF- and bFGF-induced neurite outgrowth in rat pheochromocytoma PC12 cells, but do not cause retraction of already formed neurites. Peptides which do not inhibit proteolysis are also without effect on PC12 cell differentiation. Suppression of neurite outgrowth is readily reversible upon removal of the inhibitors. These data demonstrate a requirement for specific protein turnover in the process of neuron-like differentiation in PC12 cells and provide the first demonstration of a physiological role for the N-end rule. 相似文献
83.
84.
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86.
Hieu Sy Vu Rebecca Roston Sunitha Shiva Manhoi Hur Eve Syrkin Wurtele Xuemin Wang Jyoti Shah Ruth Welti 《Plant signaling & behavior》2015,10(9)
Mechanical wounding of Arabidopsis thaliana leaves results in modifications of most membrane lipids within 6 hours. Here, we discuss the lipid changes, their underlying biochemistry, and possible relationships among activated pathways. New evidence is presented supporting the role of the processive galactosylating enzyme SENSITIVE TO FREEZING2 in the wounding response. 相似文献
87.
A novel gene coding for an endo-beta-1,4-mannanase (manA) from Bacillus subtilis strain G1 was cloned and overexpressed in P. pastoris GS115, and the enzyme was purified and characterized. The manA gene consisted of an open reading frame of 1,092 nucleotides, encoding a 364-aa protein, with a predicted molecular mass of 41 kDa. The beta-mannanase showed an identity of 90.2-92.9% (< or =95%) with the corresponding amino acid sequences from B. subtilis strains deposited in GenBank. The purified beta- mannanase was a monomeric protein on SDS-PAGE with a specific activity of 2,718 U/mg and identified by MALDITOF mass spectrometry. The recombinant beta-mannanase had an optimum temperature of 45 degrees C and optimum pH of 6.5. The enzyme was stable at temperatures up to 50 degrees C (for 8 h) and in the pH range of 5-9. EDTA and most tested metal ions showed a slightly to an obviously inhibitory effect on enzyme activity, whereas metal ions (Hg2+, Pb2+, and Co2+) substantially inhibited the recombinant beta-mannanase. The chemical additives including detergents (Triton X- 100, Tween 20, and SDS) and organic solvents (methanol, ethanol, n-butanol, and acetone) decreased the enzyme activity, and especially no enzyme activity was observed by addition of SDS at the concentrations of 0.25-1.0% (w/v) or n-butanol at the concentrations of 20-30% (v/v). These results suggested that the beta-mannanase expressed in P. pastoris could potentially be used as an additive in the feed for monogastric animals. 相似文献
88.
Nguyen Duc Quang Phan Thi Phuong Hoa Mai Sy Tuan Nguyen Xuan Viet Amadu Jalloh Hiroyuki Matsuoka 《Biochemical genetics》2009,47(5-6):370-383
The patterns of molecular evolution of the most diverse region of the apical membrane antigen 1 (AMA1) gene in Plasmodium falciparum from a Vietnamese subpopulation (Bao Loc) were investigated. Within the Bao Loc population, the sequenced gene region showed relatively high allelic and nucleotide diversity (0.985 and 0.02694, respectively). Further, the level of population recombination was substantial, resulting in a significant decay of linkage disequilibrium along the gene region. The results suggest that AMA1 is a useful genetic marker for studying the relationships between adaptation of parasite populations (to the human host immune system) and malaria epidemiology. 相似文献
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90.
Serge Mostowy Anne Danckaert To Nam Tham Christophe Machu St��phanie Guadagnini Javier Pizarro-Cerd�� Pascale Cossart 《The Journal of biological chemistry》2009,284(17):11613-11621
Septins are filament-forming GTPases implicated in several cellular
functions, including cytokinesis. We previously showed that SEPT2, SEPT9, and
SEPT11 colocalize with several bacteria entering into mammalian non-phagocytic
cells, and SEPT2 was identified as essential for this process. Here, we
investigated the function of SEPT11, an interacting partner of SEPT9 whose
function is still poorly understood. In uninfected HeLa cells, SEPT11
depletion by siRNA increased cell size but surprisingly did not affect actin
filament formation or the colocalization of SEPT9 with actin filaments. SEPT11
depletion increased Listeria invasion, and incubating SEPT11-depleted
cells with beads coated with the Listeria surface protein InlB also
led to increased entry as compared with control cells. Strikingly, as shown by
fluorescence resonance energy transfer, the InlB-mediated stimulation of Met
signaling remained intact in SEPT11-depleted cells. Taken together, our
results show that SEPT11 is not required for the bacterial entry process and
rather restricts its efficacy. Because SEPT2 is essential for the
InlB-mediated entry of Listeria, but SEPT11 is not, our findings
distinguish the roles of different mammalian septins.Septins were discovered in the budding yeast Saccharomyces
cerevisiae (1) where they
organize into a ring at the mother-bud neck during cell division
(2). Septins are GTPases of
30-65 kDa found in most eukaryotes, except plants, sharing an essential role
in cytokinesis (3,
4). Fourteen septins have been
identified in humans and classified on the basis of sequence identity into
four distinct groups (3,
5). Septins from different
groups polymerize into hetero-oligomeric protein complexes and filaments and
may associate with cellular membranes, actin filaments, and microtubules
(6,
7). Septins are increasingly
regarded as novel cytoskeletal elements
(8), but their role in
post-mitotic events remains poorly understood.The crystal structure of the SEPT2-SEPT6-SEPT7 complex recently highlighted
that septins, as opposed to actin and microtubules, form non-polar filaments
(9). In the
SEPT7-SEPT6-SEPT2-SEPT2-SEPT6-SEPT7 complex, SEPT2 has a central role in
filament formation (9), whereas
SEPT6 is thought to be replaceable with other SEPT6 group members, including
SEPT11 (3). Widely expressed in
mammalian tissues (10), SEPT11
may also be a substitute for SEPT6 in other mammalian septin complexes such as
SEPT7-SEPT9-SEPT11 (10) or
SEPT5-SEPT7-SEPT11 (11).
Because other septins homologous to SEPT11 might compensate for its deficiency
(12), the degree to which
SEPT11 is required for septin filament structure and function is not yet
known. Listeria monocytogenes is an invasive bacterium that enters
into most mammalian cells in vitro through the interaction of the
bacterial surface protein InlB with its host cellular receptor Met, the
hepatocyte growth factor receptor
(13). We originally identified
SEPT9 associated with phagosomes containing latex beads coated with InlB
(14). Given the association of
septins with the cytoskeleton, and the importance of the cytoskeleton in
bacterial invasion, we have started investigating septin function during
infection of invasive bacteria in non-phagocytic cells. We have discovered
that SEPT9, and its interacting partners SEPT2 and SEPT11, are recruited as
0.6-μm collars next to actin at the site of entry of invasive bacteria
(15). Although functional
studies using siRNA3
have revealed an essential role for SEPT2 in regulating bacterial entry, the
role of SEPT11 has not yet been investigated. We thus addressed SEPT11
function in the context of Listeria infection. 相似文献