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81.
Vrinda S. Thaker Sant Saroop Pankaj P. Vaishnav Yash Dev Singh 《Journal of Plant Growth Regulation》1986,5(1):17-27
Cytoplasmic and salt-extracted wall peroxidase and nonspecific esterase activities along with growth analysis were investigated during the entire period of cotton fiber development. Both the peroxidase fractions, when assayed with chlorogenic and ferulic acids as substrates, recorded low levels during the fiber elongation phase, and a close relationship between cessation of elongation growth and increase in peroxidase activity was discernible. Nonspecific esterase activity in both cytoplasmic and salt-extracted fractions, on the other hand, showed higher activity during the elongation phase, whereas during the secondary thickening phase it decreased. The role of cytoplasmic peroxidase in IAA oxidation is discussed. It is suggested that esterases and peroxidases associated with wall fractions may well be involved in turnover of phenolic acids that are cross-linked to wall polysaccharides. 相似文献
82.
Graeme Shannon Maria Thaker Abi Tamim Vanak Bruce R. Page Rina Grant Rob Slotow 《Ecosystems》2011,14(8):1372-1381
Elephant and fire are considered to be among the most important agents that can modify the African savanna ecosystem. Although
the synergistic relationship between these two key ecological drivers is well documented, it has proved much more difficult
to establish the relative effects they have on savanna vegetation structure at a fine-scale over time. In this study, we explore
the comparative impacts of fire and elephant on 2,522 individually identified large trees (≥5 m in height) in the Kruger National
Park, South Africa. Data were collected from 21 transects first surveyed in April 2006 and resurveyed in November 2008, to
determine the relative importance of past damage by these agents on subsequent impacts and mortality. The occurrence of fire
or elephant damage in 2006 affected the amount of tree volume subsequently removed by both these agents; elephant removed
more tree volume from previously burned trees and the impact of subsequent fire was higher on previously burned or elephant-utilized
trees than on undamaged trees. Mortality was also affected by an interaction between previous and recent damage, as the probability
of mortality was highest for trees that suffered from fire or elephant utilization after being pushed over. Subsequent fire
damage, but not elephant utilization, on debarked trees also increased the probability of mortality. Mortality was twice (4.6%
per annum) that of trees progressing into the ≥5 m height class, suggesting an overall decline in large tree density during
the 30-month study period. The responses of large trees were species and landscape-specific in terms of sensitivity to elephant
and fire impacts, as well as for levels of mortality and progression into the ≥5 m height class. These results emphasize the
need for fine-scale site-specific knowledge for effective landscape level understanding of savanna dynamics. 相似文献
83.
Sankaranarayanan Rishikesan Youg R. Thaker Ragunathan Priya Shovanlal Gayen Malathy S. S. Manimekalai Cornelia Hunke 《Molecular membrane biology》2013,30(5):400-410
A critical point in the V1 sector and entire V1VO complex is the interaction of stalk subunits G (Vma10p) and E (Vma4p). Previous work, using precipitation assays, has shown that both subunits form a complex. In this work, we have analysed the N-terminal segment of subunit G (G1–59) of the V1VO ATPase from Saccharomyces cerevisiae by using nuclear magnetic resonance (NMR) spectroscopy. Analyses of 1H-15N heteronuclear single quantum coherence (HSQC) spectra of G1–59 in the absence and presence of the N-terminal peptides E1–18 and E18–38 as well as the produced and purified C-terminal segment (E39–233) shows specific interactions only with the peptide fragment E18–38. The binding of this peptide occurs via the residues M1, V2, S3, and K5 as well for V22, S23, K24, A25 and R26 of G1–59. The specific E18–38/G1–59 binding has been confirmed by fluorescence correlation spectroscopy data. The E18–38 peptide has been studied by CD spectroscopy and NMR. The 3D structure of this peptide adopts a stable helix-hinge-helix formation in solution. A model structure of the E18–38/G1–59 complex reveals the orientation of E18–38 relative to G1–59 via salt-bridges of the polar residues and van der Waals forces at the very N-terminus of both segments. 相似文献