全文获取类型
收费全文 | 3104篇 |
免费 | 140篇 |
专业分类
3244篇 |
出版年
2018年 | 22篇 |
2017年 | 32篇 |
2016年 | 47篇 |
2015年 | 73篇 |
2014年 | 66篇 |
2013年 | 118篇 |
2012年 | 129篇 |
2011年 | 129篇 |
2010年 | 90篇 |
2009年 | 52篇 |
2008年 | 127篇 |
2007年 | 110篇 |
2006年 | 107篇 |
2005年 | 116篇 |
2004年 | 118篇 |
2003年 | 108篇 |
2002年 | 110篇 |
2001年 | 58篇 |
2000年 | 56篇 |
1999年 | 60篇 |
1998年 | 25篇 |
1997年 | 23篇 |
1996年 | 32篇 |
1995年 | 44篇 |
1994年 | 38篇 |
1993年 | 32篇 |
1992年 | 61篇 |
1991年 | 66篇 |
1990年 | 62篇 |
1989年 | 60篇 |
1988年 | 48篇 |
1987年 | 45篇 |
1986年 | 46篇 |
1985年 | 61篇 |
1984年 | 56篇 |
1983年 | 39篇 |
1982年 | 41篇 |
1981年 | 41篇 |
1980年 | 27篇 |
1979年 | 36篇 |
1978年 | 33篇 |
1977年 | 39篇 |
1976年 | 19篇 |
1975年 | 29篇 |
1974年 | 29篇 |
1973年 | 26篇 |
1971年 | 17篇 |
1970年 | 13篇 |
1969年 | 13篇 |
1968年 | 12篇 |
排序方式: 共有3244条查询结果,搜索用时 0 毫秒
51.
Models for forest ecosystem management: a European perspective 总被引:1,自引:0,他引:1
BACKGROUND: Forest management in Europe is committed to sustainability. In the face of climate change and accompanying risks, however, planning in order to achieve this aim becomes increasingly challenging, underlining the need for new and innovative methods. Models potentially integrate a wide range of system knowledge and present scenarios of variables important for any management decision. In the past, however, model development has mainly focused on specific purposes whereas today we are increasingly aware of the need for the whole range of information that can be provided by models. It is therefore assumed helpful to review the various approaches that are available for specific tasks and to discuss how they can be used for future management strategies. SCOPE: Here we develop a concept for the role of models in forest ecosystem management based on historical analyses. Five paradigms of forest management are identified: (1) multiple uses, (2) dominant use, (3) environmentally sensitive multiple uses, (4) full ecosystem approach and (5) eco-regional perspective. An overview of model approaches is given that is dedicated to this purpose and to developments of different kinds of approaches. It is discussed how these models can contribute to goal setting, decision support and development of guidelines for forestry operations. Furthermore, it is shown how scenario analysis, including stand and landscape visualization, can be used to depict alternatives, make long-term consequences of different options transparent, and ease participation of different stakeholder groups and education. CONCLUSIONS: In our opinion, the current challenge of forest ecosystem management in Europe is to integrate system knowledge from different temporal and spatial scales and from various disciplines. For this purpose, using a set of models with different focus that can be selected from a kind of toolbox according to particular needs is more promising than developing one overarching model, covering ecological, production and landscape issues equally well. 相似文献
52.
A comparative study was conducted on the effect of NH4Cl on growth, vesicle formation and formation of nitrogenase of Frankia strains Cc1.17 and Cp1.2, derived from root nodules of Colletia cruciata and Comptonia peregrina, respectively. On a medium without combined nitrogen (P-N), both strains formed spherical cells, called vesicles, like many other Frankia strains. Data are presented on the number of vesicles per mg protein, after cultivation in media with sodium propionate as C-source without combined nitrogen (P-N) or with 0.2 g NH4Cl/l (P+N). Strain Cp1.2 as may other Frankia strains, showed on P+N medium a very strong reduction of vesicle formation of 99% relative to the number of vesicles formed on P-N medium, after 11 days growth. However, in strain Cc11.17 this reduction was only 70%. The occurence of relatively large numbers of vesicles in P+N media has not yet been reported for other Frankia strains. No acetylene reduction activity was found in NH
4
+
-grown cells. The regulation of induction of nitrogenase in Frankia by NH4Cl was tested by immuno-gelectrophoresis using antisera against nitrogenase of Rhizobium leguminosarum PRE. The component I of the enzyme showed crossreactivity while the component II had only a weak crossreaction. The experiments indicated that no nitrogenase was detectable in the NH
4
+
-grown cells. For the localization of nitrogenase, relative amounts of the enzyme were compared in whole cells and vesicle-enriched fractions. Western blots showed a significant enrichment of nitrogenase in the vesicle fractions, which indicated that most of the nitrogenase was localized in the vesicle. 相似文献
53.
By means of thin layer chromatography in conjunction with mass spectrometry the pyrrolizidine alkaloid patterns derived fromSymphytum asperum, several cytotypes ofS. officinale agg. and the artificial hybrids of the former taxa, were compared. The obtained patterns were not essentially affected by
variation in cytotype, harvesting times and -location of plants. Lycopsamine, acetyl-lycopsamine and symphytine or their isomers
were generally found in theS. officinale cytotypes, echimidine and symphytine inS. asperum. The interspecific hybrids contained all alkaloids mentioned. The definite lack of echimidine in the 2 n=40 cytotype proves
that it is conspecific withS. officinale and does not belong to a hybrid-swarmS. asperum × S. officinale with 2 n=48.
Part I of this series of contributions. 相似文献
54.
Cell polarity development and protein trafficking in hepatocytes lacking E-cadherin/beta-catenin-based adherens junctions 下载免费PDF全文
Théard D Steiner M Kalicharan D Hoekstra D van Ijzendoorn SC 《Molecular biology of the cell》2007,18(6):2313-2321
Using a mutant hepatocyte cell line in which E-cadherin and beta-catenin are completely depleted from the cell surface, and, consequently, fail to form adherens junctions, we have investigated adherens junction requirement for apical-basolateral polarity development and polarized membrane trafficking. It is shown that these hepatocytes retain the capacity to form functional tight junctions, develop full apical-basolateral cell polarity, and assemble a subapical cortical F-actin network, although with a noted delay and a defect in subsequent apical lumen remodeling. Interestingly, whereas hepatocytes typically target the plasma membrane protein dipeptidyl peptidase IV first to the basolateral surface, followed by its transcytosis to the apical domain, hepatocytes lacking E-cadherin-based adherens junctions target dipeptidyl peptidase IV directly to the apical surface. Basolateral surface-directed transport of other proteins or lipids tested was not visibly affected in hepatocytes lacking E-cadherin-based adherens junctions. Together, our data show that E-cadherin/beta-catenin-based adherens junctions are dispensable for tight junction formation and apical lumen biogenesis but not for apical lumen remodeling. In addition, we suggest a possible requirement for E-cadherin/beta-catenin-based adherens junctions with regard to the indirect apical trafficking of specific proteins in hepatocytes. 相似文献
55.
M. Dürr K. Urech Th. Boller A. Wiemken J. Schwencke M. Nagy 《Archives of microbiology》1979,122(2):169-175
The conditions for synthesis, purification, and properties of tryptophanase by a marine organism (Vibrio K-7) were studied. Tryptophanase was induced by tryptophan and its analogs, and partially repressed by 0.5% glucose or glycerol. NaCl (0.4M) was required for optimal growth and tryptophanase activity in whole cells. The enzyme was purified to 92% homogeneity by heat treatment, hydroxyapatite chromatography and fractionation with ammonium sulfate. This tryptophanase has been found to have kinetic properties similar to the tryptophanase from other microorganisms. It carries out both , -elimination reactions (using tryptophan, serine, cysteine and S-methyl-cysteine as substrates) and -replacement reactions (forming tryptophan from indole and serine, cysteine or S-methyl-cysteine). The enzyme has a sedimentation coefficient of 9.2S and requires pyridoxal 5-phosphate as a cofactor. The optimal pH for the tryptophanase reaction is pH 8.0.Nonstandard Abbreviations PLP
pyridoxal 5-phosphate
- TPase
tryptophanase
- TSase
tryptophan synthase
- DHase
dehydratase
- TCA
tricarboxylic acid
- BSA
bovine serum albumin
Preliminary reports of this work have been presented (M. J. Klug and R. D. DeMoss, Bacteriol. Proc. 1971, p. 132; D. D. Whitt and R. D. DeMoss, Abstr. Annu. Meet. Am. Soc. Microbiol. 1973, p. 148) 相似文献
56.
William?H.?Morgan Elisa?Thébault Colleen?L.?Seymour F.?J.?Frank?van?VeenEmail author 《BioControl》2017,62(2):175-184
Host–parasitoid dynamics are intrinsically unstable unless the risk of parasitism is sufficiently heterogeneous among hosts. Spatial aggregation of parasitoids can contribute to this heterogeneity, stabilising host–parasitoid population dynamics and thereby reducing pest outbreaks. We examined the spatial distribution of mango gall fly (Procontarinia matteiana, Kiefer and Cecconi), a non-native pest of South African mango orchards, which is controlled by a single parasitoid (Chrysonotomyia pulcherrima, Kerrich). We assessed whether spatial aggregation of parasitoids is associated with proximity to natural vegetation and/or to host density-dependent and host density-independent factors at three spatial scales. We found evidence for higher parasitism rates near natural vegetation at the field scale, and inverse host-density dependent and density-independent parasitoid aggregation at both the leaf scale and field scale. Therefore, we conclude that natural vegetation plays a role in promoting stabilising aggregation of parasitoids, possibly through provision of non-host resources (nectar, pollen), in this system. 相似文献
57.
58.
El-Murr N Abidi Z Wanherdrick K Svrcek M Gaub MP Fléjou JF Hamelin R Duval A Lesuffleur T 《PloS one》2012,7(2):e31862
Mismatch repair-deficient colorectal cancers (CRC) display widespread instability at DNA microsatellite sequences (MSI). Although MSI has been reported to commonly occur at coding repeats, leading to alterations in the function of a number of genes encoding cancer-related proteins, nothing is known about the putative impact of this process on non-coding microRNAs. In miRbase V15, we identified very few human microRNA genes with mono- or di-nucleotide repeats (n = 27). A mutational analysis of these sequences in a large series of MSI CRC cell lines and primary tumors underscored instability in 15 of the 24 microRNA genes successfully studied at variable frequencies ranging from 2.5% to 100%. Following a maximum likelihood statistical method, microRNA genes were separated into two groups that differed significantly in their mutation frequencies and in their tendency to represent mutations that may or may not be under selective pressures during MSI tumoral progression. The first group included 21 genes that displayed no or few mutations in CRC. The second group contained three genes, i.e., hsa-mir-1273c, hsa-mir-1303 and hsa-mir-567, with frequent (≥ 80%) and sometimes bi-allelic mutations in MSI tumors. For the only one expressed in colonic tissues, hsa-mir-1303, no direct link was found between the presence or not of mono- or bi-allelic alterations and the levels of mature miR expression in MSI cell lines, as determined by sequencing and quantitative PCR respectively. Overall, our results provide evidence that DNA repeats contained in human miRNA genes are relatively rare and preserved from mutations due to MSI in MMR-deficient cancer cells. Functional studies are now required to conclude whether mutated miRNAs, and especially the miR-1303, might have a role in MSI tumorigenesis. 相似文献
59.
In 1980, the seasonal changes in nitrogen and phosphorus concentration of various plant parts of Nymphoides peltata (Gmel.) O. Kuntze, together with aspects of nitrogen and phosphorus cycling by this species were studied in an oxbow lake of the river Waal (The Netherlands). The nitrogen and phosphorus stores of the water, seston, sediment and macrophyte compartments were assessed each month.The underground Nymphoides structures had high nitrogen and phosphorus concentrations before and after the main growing season, while during summer the aboveground plant parts had high nutrient contents. Nymphoides peltata accumulated maximum amounts of nitrogen (334 mmol m−2) and phosphorus (56.6 mmol m−2) in July. The upper layers of the bottom appeared to be an enormous nutrient reservoir (94–99% of total) of which the largest part was not directly available to Nymphoides. Nutrient uptake from the sediments by N. peltata is suggested by the fact that the bottom and/or interstitial water of the sample station devoid of rooted macrophytes, contained higher concentrations of nitrogen and phosphorus than that of the Nymphoides stands. The annual flux of nutrients from Nymphoides to the detritus compartment was estimated to be ca. 1200 mmol nitrogen and 164 mmol phosphorus per m2 of littoral. During breakdown of the detritus there was a relatively fast net conversion of organically bound nitrogen and phosphorus to inorganic forms, especially at higher temperatures.Nymphoides has the potential to function as an important nitrogen and phosphorus pump, which regenerates sediment nutrients. 相似文献