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991.
Tetsuya Akamatsu Shigeo Daikoku Hideaki Nagamune Shigeru Yoshida Kenji Mori Akihiko Tsuji Y. Matsuda 《Histochemistry and cell biology》1997,108(2):95-103
PACE4 is a mammalian Kexin family protease that is involved in the maturation of precursor proteins. Four PACE4 isoforms
have been identified. We identified a novel PACE4 isoform, PACE4E, from a human cerebellum cDNA library, which possesses a
hydrophobic cluster in its C-terminus participating in membrane association. The size of PACE4E mRNA from adult rat brain
was estimated by Northern blotting to be 4.4 kb. In situ hybridization histochemistry revealed that the highest level of PACE4E
mRNA was expressed in the mitral cells of the adult rat olfactory bulb (OB). The OB is a unique sensory organ in that it has
a lifelong regenerating capacity and it affects brain development. We further analyzed the expression of PACE4E mRNA in the
developing olfactory system. On day 13.5 of gestation, PACE4E mRNA was expressed at high levels in the neuroepithelium of
the forebrain vesicle (FV), olfactory epithelium, and cells in the fiber bundles projecting to the FV. As development proceeded,
PACE4E mRNA was expressed in developing mitral cells but decreased in the olfactory epithelium. In the newborn, its expression
was confined to the mitral cells in both the main and accessory OB and in some periglomerular cells, as shown in adult rats.
The spatio-temporal expression of PACE4E suggests that it plays a role in the establishment and maintenance of the olfactory
receptor system.
Accepted: 15 April 1997 相似文献
992.
Masayuki Ezoe Toru Minami Yutaro Ogawa Shigeyuki Yagi Hiroyuki Nakazumi Tetsuya Matsuyama Kenji Wada Hiromichi Horinaka 《Photochemical & photobiological sciences》2005,4(8):641-646
Structurally controlled zinc porphyrin-anthracene dyads, syn-arranged 1 and anti-arranged 2, were newly synthesized employing a diarylurea linkage, and the excitation energy transfer (EET) from the anthracene to the zinc porphyrin chromophore was investigated by steady-state fluorescence emission spectroscopy as well as fluorescence lifetime measurement, especially focusing on the effect of the chromophoric orientation on the EET. In both of the dyads, intramolecular EET was facilitated upon excitation of the anthracene chromophore (lamda(ex)= 401 nm), and the zinc porphyrin S1-S0 emission (580-720 nm) was enhanced. The EET in the syn-arranged dyad 1 was more efficient than in the anti-arranged 2: the S1-S0 emission in 1 was 1.8 times larger than that in the zinc porphyrin reference compound 3, whereas that in 2 was enhanced by 1.6 times, compared to that in 3. In the fluorescence lifetime measurement, the quiet short-lived component assignable to the EET was observed for the dyads 1 and 2 beyond the analysis limit (<25 ps). The EET rate constants in the dyads 1 and 2 were estimated as not less than 4.0 x 10(10) s-1. However, in the case of 2, the residual long-lived component assigned to the anthracene emission was also observed at 425 nm. These results showed that the syn-arrangement of the zinc porphyrin and anthracene chromophores was more preferred for intramolecular EET to the anti-arrangement. 相似文献
993.
Atsuko Kushi Kiyotaka Akiyama Masato Noguchi Koji Edamura Takayuki Yoshida Hitoshi Sasai 《Experimental Animals》2004,53(5):437-443
Alpha-1-antitrypsin (alpha1-AT) is a member of the serine protease inhibitor family regulating numerous proteolytic processes. The genetic disorder, alpha1-AT deficiency, is well known as a cause of hereditary pulmonary emphysema and liver cirrhosis. To create an animal model of human alpha1-AT deficiency, we disrupted the major murine isoform PI2, which is similar to human alpha1-AT and is one of 7 alpha1-AT isoforms found in the mouse. The ability of the serum to inhibit the activities of human leukocyte elastase (HLE) and human chymotrypsin (CYT) was significantly lower in heterozygous mice (alpha1-AT/PI2 -/+) than wild-type (alpha1-AT/PI2 +/+) mice (73.2% vs. 100% for HLE and 67.8% vs.100% for CYT, respectively; P<0.05). The distribution of genotypes among F(2) progeny was not in accordance with Mendelian distribution (P<0.01), as the percentages of wild-type, heterozygotes and homozygotes were 47.8%, 37.3% and 14.9%, respectively. Thus, it is likely that impairment of the protease inhibitor had a critical effect on fetus development. The alpha1-AT/PI2 deficient mouse will be a useful animal model for elucidating the function of alpha1-AT in fetal development, studying the mechanisms of chronic inflammatory disease and evaluating therapeutic candidates for the treatment of inflammatory disease. 相似文献
994.
Mariko Naito Yoshitoshi Ogura Takehiko Itoh Mikio Shoji Masaaki Okamoto Tetsuya Hayashi Koji Nakayama 《DNA research》2016,23(1):11-19
Prevotella intermedia is a pathogenic bacterium involved in periodontal diseases. Here, we present the complete genome sequence of a clinical strain, OMA14, of this bacterium along with the results of comparative genome analysis with strain 17 of the same species whose genome has also been sequenced, but not fully analysed yet. The genomes of both strains consist of two circular chromosomes: the larger chromosomes are similar in size and exhibit a high overall linearity of gene organizations, whereas the smaller chromosomes show a significant size variation and have undergone remarkable genome rearrangements. Unique features of the Pre. intermedia genomes are the presence of a remarkable number of essential genes on the second chromosomes and the abundance of conjugative and mobilizable transposons (CTns and MTns). The CTns/MTns are particularly abundant in the second chromosomes, involved in its extensive genome rearrangement, and have introduced a number of strain-specific genes into each strain. We also found a novel 188-bp repeat sequence that has been highly amplified in Pre. intermedia and are specifically distributed among the Pre. intermedia-related species. These findings expand our understanding of the genetic features of Pre. intermedia and the roles of CTns and MTns in the evolution of bacteria. 相似文献
995.
Hattan Jun-ichiro Shindo Kazutoshi Ito Tomoko Shibuya Yurica Watanabe Arisa Tagaki Chie Ohno Fumina Sasaki Tetsuya Ishii Jun Kondo Akihiko Misawa Norihiko 《Planta》2016,243(4):959-972
Planta - A novel terpene synthase ( Tps ) gene isolated from Camellia brevistyla was identified as hedycaryol synthase, which was shown to be expressed specifically in flowers. Camellia plants are... 相似文献
996.
997.
Kaho Nishizawa Tetsuya Masuda Yasuyuki Takenaka Hironori Masui Fumito Tani 《Bioscience, biotechnology, and biochemistry》2016,80(8):1623-1631
Sword bean (Canavalia gladiata) seeds are a traditional food in Asian countries. In this study, we aimed to determine the optimal methods for the precipitation of sword bean proteins useful for the food development. The soaking time for sword beans was determined by comparing it with that for soybeans. Sword bean proteins were extracted from dried seeds in distilled water using novel methods. We found that most proteins could be precipitated by heating the extract at more than 90 °C. Interestingly, adding magnesium chloride to the extract at lower temperatures induced specific precipitation of a single protein with a molecular weight of approximately 48 kDa. The molecular weight and N-terminal sequence of the precipitated protein was identical to that of canavalin. These data suggested that canavalin was precipitated by the addition of magnesium chloride to the extract. Our results provide important insights into the production of processed foods from sword bean. 相似文献
998.
999.
Futoshi Suizu Noriyuki Hirata Kohki Kimura Tatsuma Edamura Tsutomu Tanaka Satoko Ishigaki Thoria Donia Hiroko Noguchi Toshihiko Iwanaga Masayuki Noguchi 《The EMBO journal》2016,35(12):1346-1363
A primary cilium is a microtubule‐based sensory organelle that plays an important role in human development and disease. However, regulation of Akt in cilia and its role in ciliary development has not been demonstrated. Using yeast two‐hybrid screening, we demonstrate that Inversin (INVS) interacts with Akt. Mutation in the INVS gene causes nephronophthisis type II (NPHP2), an autosomal recessive chronic tubulointerstitial nephropathy. Co‐immunoprecipitation assays show that Akt interacts with INVS via the C‐terminus. In vitro kinase assays demonstrate that Akt phosphorylates INVS at amino acids 864–866 that are required not only for Akt interaction, but also for INVS dimerization. Co‐localization of INVS and phosphorylated form of Akt at the basal body is augmented by PDGF‐AA. Akt‐null MEF cells as well as siRNA‐mediated inhibition of Akt attenuated ciliary growth, which was reversed by Akt reintroduction. Mutant phosphodead‐ or NPHP2‐related truncated INVS, which lack Akt phosphorylation sites, suppress cell growth and exhibit distorted lumen formation and misalignment of spindle axis during cell division. Further studies will be required for elucidating functional interactions of Akt–INVS at the primary cilia for identifying the molecular mechanisms underlying NPHP2. 相似文献
1000.
The Restorer‐of‐fertility‐like 2 pentatricopeptide repeat protein and RNase P are required for the processing of mitochondrial orf291 RNA in Arabidopsis
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