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991.
Jun-Ichi Nakamura Shin Yazawa Toshikazu Hada Takayuki Asao Hiroshi Naitoh Seiichi Takenoshita Masaaki Kosaka Suguru Akamatsu Tetsuya Tachikawa Yukio Nagamachi 《Glycoconjugate journal》1997,14(1):81-87
Levels of fucosylated antigens in sera from patients with liver diseases were examined by a newly developed sandwich-type enzyme immuno assay with the aid of anti-fucosylated antigen antibody, YB-2 which reacts simultaneously with Y, Leb and H type 2 antigens. When the cut-off value was set arbitrarily at mean [3 SD values of normal, 30 (69.8%) of the 43 patients with HCC, 14 (53.8%) of the 26 patients with liver cirrhosis (LC) and 24 (45.3%) of the 53 patients with chronic hepatitis (CH) were found to be positive, whereas all of the 30 samples from healthy controls were negative. The levels of -fetoprotein (AFP) and protein induced by vitamin K absence or antagonist-II (PIVKA-II) in HCC were not correlated with those of YB-2 antigens. The positive rates of the combination YB-2 and AFP assay and YB-2 and PIVKA-II assay in HCC were significantly higher (83.7 and 86.0%, respectively) than that of the AFP and PIVKA-II combination (65.1%) which had been reported to be the best combination up to this time. 相似文献
992.
A linear analogue network model is proposed to describe the neuronal circuit of the outer retina consisting of cones, horizontal
cells, and bipolar cells. The model reflects previous physiological findings on the spatial response properties of these neurons
to dim illumination and is expressed by physiological mechanisms, i.e., membrane conductances, gap-junctional conductances,
and strengths of chemical synaptic interactions. Using the model, we characterized the spatial filtering properties of the
bipolar cell receptive field with the standard regularization theory, in which the early vision problems are attributed to
minimization of a cost function. The cost function accompanying the present characterization is derived from the linear analogue
network model, and one can gain intuitive insights on how physiological mechanisms contribute to the spatial filtering properties
of the bipolar cell receptive field. We also elucidated a quantitative relation between the Laplacian of Gaussian operator
and the bipolar cell receptive field. From the computational point of view, the dopaminergic modulation of the gap-junctional
conductance between horizontal cells is inferred to be a suitable neural adaptation mechanism for transition between photopic
and mesopic vision.
Received: 20 December 1996 / Accepted in revised form: 16 May 1997 相似文献
993.
Involvement of the protein of Xenopus vasa homolog (Xenopus vasa-like gene 1, XVLG1) in the differentiation of primordial germ cells 总被引:1,自引:0,他引:1
In order to understand the role of the protein of Xenopus vasa homolog ( Xenopus vasa -like gene 1, XVLG1 ) in germ line cells, an attempt was made to perturb the function of the protein with the anti-vasa antibody 2L-13. The 2L-13 or the control antibody was microinjected with a lineage tracer (FITC-dextran-lysine, FDL) into single vegetal blastomeres containing the germ plasm of Xenopus 32-cell embryos, the descendants of which were destined to differentiate into a small number of primordial germ cells (PGC) and a large number of somatic cells, mostly of endodermal tissues at the tadpole stage. No significant effect of the injection of the antibodies on FDL-labeled, presumptive PGC (pPGC) was observed in embryos until stage 37/38. However, FDL-labeled PGC were not observed in almost all the 2L-13 antibody-injected tadpoles, although a similar number of labeled somatic cells were always present. As 2L-13 antibody specifically reacts with XVLG1 protein in the embryos by immunoblotting, the present results suggest that the antibody perturbed the function of XVLG1 protein in the pPGC, resulting in failure of PGC differentiation at the tadpole stage. 相似文献
994.
Recording of mitochondrial transmembrane potential and volume in cultured rat osteoclasts by confocal laser scanning microscopy 总被引:2,自引:0,他引:2
H. Fujii S. H. Cody U. Seydel J. M. Papadimitriou D. J. Wood M.-H. Zheng 《Journal of molecular histology》1997,29(8):571-581
Osteoclasts are multinuclear bone-resorbing cells which contain abundant mitochondria. Morphological studies have suggested
that a correlation may exist between mitochondrial concentration and bone resorption by osteoclasts. However, investigation
of mitochondrial transmembrane potential (Delta rho) and volume has been hampered by the difficulty in obtaining a sufficient
number of osteoclasts for assessing these characteristics by flow cytometric analysis. In this study, we have used confocal
laser scanning microscopy after loading the cells with Rhodamine 123 and 10-nonyl Acridine Orange to record mitochondrial
Delta rho and volume, respectively, in isolated rat osteoclasts cultured on bovine bone slices. Optimal staining conditions
were found to be 10 mu g ml-1 for 40 min for Rhodamine, and 1 mu S mD for 10 min for the 10-nonyl Acridine Orange derivative.
Two osteoclast populations, whose shape seemed to reflect bone resorption and migratory functions, were identified depending
on their shape and on the distribution of the two dye probes. ‘Round-shaped’ osteoclasts had significantly higher mitochondrial
Delta rho and volume in the apical regions than in the basolateral portions (p 0.00001). In contrast, mitochondrial Delta
rho and volume in ‘irregular- shaped’ osteoclasts were rather evenly distributed in both these regions (p > 0.05). Our results
indicate that there is an apical polarization of mitochondria in osteoclasts corresponding to the energy demands associated
with bone resorption.
This revised version was published online in November 2006 with corrections to the Cover Date. 相似文献
995.
Teiji Takechi Katsuhisa Koizumi Atsushi Azuma Masakazu Fukushima Katsutoshi Kobayashi Shinya Oda Katsuhiko Yanaga Leon Mullenders Peter Karran Masatsugu Ueda Yoshito Terai Minoru Ueki Masaru Sakamoto Aako Kondo Kiyohiko Miyake Yauko Koyamatsu Tsukasa Akiya Makoto Nakano Hiroshi Iwabuchi Tetsuya Muroya Yoshio Tenjin Kazunori Ochiai Tadao Tanaka Kyosuke Ymada Kazu Ueda Akihiko Misawa Aikou Okamoto Eizo Kimura Makoto Yasuda 《Human cell》2004,17(2):16-21
996.
997.
R J Castellani P L Harris L M Sayre J Fujii N Taniguchi M P Vitek H Founds C S Atwood G Perry M A Smith 《Free radical biology & medicine》2001,31(2):175-180
Advanced glycation end products are a diverse class of posttranslational modifications, stemming from reactive aldehyde reactions, that have been implicated in the pathogenesis of a number of degenerative diseases. Because advanced glycation end products are accelerated by, and result in formation of, oxygen-derived free radicals, they represent an important component of the oxidative stress hypothesis of Alzheimer disease (AD). In this study, we used in situ techniques to assess N(epsilon)-(Carboxymethyl)lysine (CML), the predominant advanced glycation end product that accumulates in vivo, along with its glycation-specific precursor hexitol-lysine, in patients with AD as well as in young and aged-matched control cases. Both CML and hexitol-lysine were increased in neurons, especially those containing intracellular neurofibrillary pathology in cases of AD. The increase in hexitol-lysine and CML in AD suggests that glycation is an early event in disease pathogenesis. In addition, because CML can result from either lipid peroxidation or advanced glycation, while hexitol-lysine is solely a product of glycation, this study, together with studies demonstrating the presence of 4-hydroxy-2-nonenal adducts and pentosidine, provides evidence of two distinct oxidative processes acting in concert in AD neuropathology. Our findings support the notion that aldehyde-mediated modifications, together with oxyradical-mediated modifications, are critical pathogenic factors in AD. 相似文献
998.
999.
Makoto J Tabata Tatsushi Matsumura Takafumi Fujii Makoto Abe Kojiro Kurisu 《The journal of histochemistry and cytochemistry》2003,51(12):1673-1679
During tooth development, the growth and differentiation of ameloblast lineage (AL) cells are regulated by epithelial-mesenchymal interactions. To examine the dynamic effects of components of the basement membrane, which is the extracellular matrix (ECM) lying between the epithelium and mesenchyme, we prepared AL cells from the epithelial layer sheet of mandibular incisors of postnatal day 7 rats and cultured them on plates coated with type IV collagen, laminin-1, or fibronectin. The growth of AL cells was supported by type IV collagen and fibronectin but not by laminin-1 in comparison with that on type I collagen as a reference. Clustering and differentiation of AL cells were observed on all matrices examined. AL cells showed normal growth and differentiation at low cell density on fibronectin but not on type I collagen. Furthermore, the population of cytokeratin 14-positive cells on fibronectin was lower than that on other ECM components, suggesting that fibronectin may be a modulator to accelerate the differentiation of AL cells. After the cells had been cultured for 9 days on fibronectin, crystal-like structures were observed. These structures overlaid the cell clusters and were positive for von Kossa staining. These findings indicate that each matrix component has a regulative role in the proliferation and differentiation of AL cells and that fibronectin causes the greatest acceleration of AL cell differentiation. 相似文献
1000.