全文获取类型
收费全文 | 2178篇 |
免费 | 161篇 |
国内免费 | 2篇 |
专业分类
2341篇 |
出版年
2022年 | 19篇 |
2021年 | 26篇 |
2020年 | 12篇 |
2019年 | 20篇 |
2018年 | 26篇 |
2017年 | 33篇 |
2016年 | 47篇 |
2015年 | 73篇 |
2014年 | 81篇 |
2013年 | 120篇 |
2012年 | 143篇 |
2011年 | 137篇 |
2010年 | 70篇 |
2009年 | 76篇 |
2008年 | 133篇 |
2007年 | 127篇 |
2006年 | 118篇 |
2005年 | 109篇 |
2004年 | 118篇 |
2003年 | 96篇 |
2002年 | 99篇 |
2001年 | 46篇 |
2000年 | 39篇 |
1999年 | 50篇 |
1998年 | 17篇 |
1997年 | 25篇 |
1996年 | 15篇 |
1995年 | 28篇 |
1994年 | 28篇 |
1993年 | 17篇 |
1992年 | 37篇 |
1991年 | 28篇 |
1990年 | 28篇 |
1989年 | 38篇 |
1988年 | 39篇 |
1987年 | 25篇 |
1986年 | 31篇 |
1985年 | 27篇 |
1984年 | 26篇 |
1983年 | 12篇 |
1982年 | 28篇 |
1981年 | 10篇 |
1980年 | 9篇 |
1979年 | 6篇 |
1978年 | 7篇 |
1977年 | 5篇 |
1974年 | 4篇 |
1973年 | 4篇 |
1970年 | 5篇 |
1969年 | 5篇 |
排序方式: 共有2341条查询结果,搜索用时 0 毫秒
971.
972.
Multiple Contributions of Peroxisomal Metabolic Function to Fungal Pathogenicity in Colletotrichum lagenarium 下载免费PDF全文
In Colletotrichum lagenarium, which is the causal agent of cucumber anthracnose, PEX6 is required for peroxisome biogenesis and appressorium-mediated infection. To verify the roles of peroxisome-associated metabolism in fungal pathogenicity, we isolated and functionally characterized ICL1 of C. lagenarium, which encodes isocitrate lyase involved in the glyoxylate cycle in peroxisomes. The icl1 mutants failed to utilize fatty acids and acetate for growth. Although Icl1 has no typical peroxisomal targeting signals, expression analysis of the GFP-Icl1 fusion protein indicated that Icl1 localizes in peroxisomes. These results indicate that the glyoxylate cycle that occurs inside the peroxisome is required for fatty acid and acetate metabolism for growth. Importantly, in contrast with the pex6 mutants that form nonmelanized appressoria, the icl1 mutants formed appressoria that were highly pigmented with melanin, suggesting that the glyoxylate cycle is not essential for melanin biosynthesis in appressoria. However, the icl1 mutants exhibited a severe reduction in virulence. Appressoria of the icl1 mutants failed to develop penetration hyphae in the host plant, suggesting that ICL1 is involved in host invasion. The addition of glucose partially restored virulence of the icl1 mutant. Heat shock treatment of the host plant also enabled the icl1 mutants to develop lesions, implying that the infection defect of the icl1 mutant is associated with plant defense. Together with the requirement of PEX6 for appressorial melanization, our findings suggest that peroxisomal metabolic pathways play functional roles in appressorial melanization and subsequent host invasion steps, and the latter step requires the glyoxylate cycle. 相似文献
973.
Fujita K Yamaguchi Y Mori T Muramatsu N Miyamoto T Yano M Miyata H Ootsuyama A Sawada M Matsuda H Kaji R Sakaguchi S 《Cellular and molecular neurobiology》2011,31(7):999-1008
We first verified that a single chain Fv fragment against prion protein (anti-PrP scFv) was secreted by HEK293T cells and prevented prion replication in infected cells. We then stably expressed anti-PrP scFv in brain-engraftable murine microglial cells and intracerebrally injected these cells into mice before or after infection with prions. Interestingly, the injection before or at an early time point after infection attenuated the infection marginally but significantly prolonged survival times of the mice. These suggest that the ex vivo gene transfer of anti-PrP scFvs using brain-engraftable cells could be a possible immunotherapeutic approach against prion diseases. 相似文献
974.
975.
NSm and 78-kilodalton proteins of Rift Valley fever virus are nonessential for viral replication in cell culture 总被引:1,自引:0,他引:1 下载免费PDF全文
Rift Valley fever viruses carrying mutations of the M gene preglycoprotein region, one lacking NSm protein expression, one lacking 78-kDa protein expression, and one lacking expression of both proteins, were compared in cell culture. All of the mutants and their parent virus produced plaques with similar sizes and morphologies in Vero E6 cells and had similar growth kinetics in Vero, C6/36, and MRC5 cells, demonstrating that the NSm and 78-kDa proteins were not needed for the virus to replicate efficiently in cell culture. A competition-propagation assay revealed that the parental virus was slightly more fit than the mutant virus lacking expression of both proteins. 相似文献
976.
977.
Alpha‐2 macroglobulin as a region‐specific secretory protein in male reproductive tract,and its dynamics during sperm transit toward the female spermatheca in the blue crab 下载免费PDF全文
978.
979.
Paroxysmal nocturnal haemoglobinuria (PNH) is caused by somatic mutations in the PIG-A gene. 总被引:17,自引:1,他引:16 下载免费PDF全文
M Bessler P J Mason P Hillmen T Miyata N Yamada J Takeda L Luzzatto T Kinoshita 《The EMBO journal》1994,13(1):110-117
Paroxysmal nocturnal haemoglobinuria (PNH), an acquired clonal blood disorder, is caused by the absence of glycosyl phosphatidylinositol (GPI)-anchored surface proteins due to a defect in a specific step of GPI-anchor synthesis. The cDNA of the X-linked gene, PIG-A, which encodes a protein required for this step has recently been isolated. We have carried out a molecular and functional analysis of the PIG-A gene in four cell lines deficient in GPI-linked proteins, obtained by Epstein-Barr virus (EBV) transformation of affected B-lymphocytes from PNH patients. In all four cell lines transfection with PIG-A cDNA restored normal expression of GPI-linked proteins. In three of the four cell lines the primary lesion is a frameshift mutation. In two of these there is a reduction in the amount of full-length mRNA. The fourth cell line contains a missense mutation in PIG-A. In each case the mutation was present in the affected granulocytes from peripheral blood of the patients, but not in normal sister cell lines from the same patient. These data prove that PNH is caused in most patients by a single mutation in the PIG-A gene. The nature of the mutation can vary and most likely occurs on the active X-chromosome in an early haematopoietic stem cell. 相似文献
980.
Pyrrolidine, one of biogenic volatile amines, possesses nicotine-like synaptotropic actions on the nervous systems. In the present study, pyrrolidine levels in the tissues were examined by using mass fragmentographic technique. High concentrations of pyrrolidine were found in the seminal vesicle and lung of rabbits. Only trace amounts of pyrrolidine existed in the brain of mice and rats, although higher concentrations were detected in the brain of rabbits. In the rat brain, however, high levels of pyrrolidine were found in the pineal gland, pituitary gland and corpus striatum. 相似文献