全文获取类型
收费全文 | 380篇 |
免费 | 22篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2021年 | 5篇 |
2020年 | 9篇 |
2019年 | 6篇 |
2018年 | 9篇 |
2017年 | 10篇 |
2016年 | 13篇 |
2015年 | 20篇 |
2014年 | 21篇 |
2013年 | 26篇 |
2012年 | 33篇 |
2011年 | 33篇 |
2010年 | 33篇 |
2009年 | 19篇 |
2008年 | 30篇 |
2007年 | 30篇 |
2006年 | 19篇 |
2005年 | 19篇 |
2004年 | 19篇 |
2003年 | 12篇 |
2002年 | 14篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 6篇 |
1998年 | 3篇 |
1997年 | 4篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1986年 | 1篇 |
排序方式: 共有402条查询结果,搜索用时 281 毫秒
1.
J. Brian Parent Tet-Kin Yeol Kiang-Teck Yeol Kenneth Olden 《Molecular and cellular biochemistry》1986,72(1-2):21-33
To investigate our earlier hypothesis that carbohydrates play a regulatory role in the intracellular transport of secretory glycoproteins, we used 1-deoxynojirimycin (DNJ), and inhibitor of glucosidase I and II of the rough endoplasmic reticulum (RER), to modify the structure of N-linked glycan moieties of secretory glycoproteins of human hepatoma (Hep G2) cells in culture. Using a pulse-chase protocol, we found that treatment of Hep G2 cultures with 1.25 mM DNJ markedly reduced the rate of secretion of 1-protease inhibitor, ceruloplasmin, and 2-macroglobulin, but had no effect on the export of fibronectin, -fetoprotein and transferrin, nor on albumin which lacks carbohydrate. For example, 50% of newly synthesized 1-protease inhibitor, the glycoprotein most dramatically affected, was secreted by 27 min in control cultures versus 110 min in DNJ-treated cultures. Percoll gradient cell fractionation analyses revealed that DNJ inhibited transport of the affected secretory glycoproteins in the RER segment of the ER/Golgi pathway. For example, 50% of newly synthesized 1-protease inhibitor was lost from the RER fraction by 10 min in untreated cells, but 70 min was required for the transport of a similar amount of protein in DNJ-treated cells. DNJ treatment also inhibited the rate at which the N-linked glycan moieties of the affected glycoproteins became resistant to endo H in the Golgi. Since the glycan moiety of secreted forms of the affected glycoproteins were fully processed to the complex structure, suggesting escape from DNJ inhibition, we concluded that removal of terminal glucose residues from the glycan chain of secretory glycoproteins is required for their transport from the RER to the Golgi. We suggest that the oligosaccharide moieties on 1-protease inhibitor, ceruloplasmin and 2-macroglobulin form part of the binding site for a receptor which regulates transport of these glycoproteins. 相似文献
2.
3.
Anh Duc Nguyen In Yeub Hwang Jeon Young Chan Eun Yeol Lee 《Biotechnology and Bioprocess Engineering》2016,21(4):477-482
One-carbon feedstock such as methanol and formate has attracted much attention as carbon substrate of industrial biotechnology for production of value-added chemicals and biofuels. Productivity improvement of natural one-carbon metabolic pathways in native hosts such as methanotrophs is somewhat difficult due to inefficient genetic tools and low specific growth rate. As an alternative, metabolic engineering can create new and efficient metabolic pathways of one-carbon substrate that can be readily transferred to non-native hosts. In this paper, recent progresses in protein and metabolic engineering for creation of methanol and formate-utilizing synthetic pathways based on RuMP cycle and formolase are reviewed. Perspectives on one-carbon metabolic pathway engineering in non-native host are also discussed. 相似文献
4.
Hee Jin Park Hyeong Cheol Park Sang Yeol Lee Hans J. Bohnert Dae-Jin Yun 《Journal of Plant Biology》2011,54(5):275-285
Posttranslational modifications of proteins by small polypeptides including ubiquitination, neddylation (related to ubiquitin
(RUB) conjugation), and sumoylation are implicated in plant growth and development, and they regulate protein degradation,
location, and interaction with other proteins. Ubiquitination mediates the selective degradation of proteins by the ubiquitin
(Ub)/proteasome pathway. The ubiquitin-like protein RUB is conjugated to cullins, which are part of a ubiquitin E3 ligase
complex that is involved in auxin hormonal signaling. Sumoylation, by contrast, is known for its involvement in guiding protein
interactions related to abiotic and biotic stresses and in the regulation of flowering time. ATG8/ATG12-mediated autophagy
influences degradation and recycling of cellular components. Other ubiquitin-like modifiers (ULPs) such as homology to Ub-1,
ubiquitin-fold modifier 1, and membrane-anchored Ub-fold are also found in Arabidopsis. ULPs share similar three-dimensional structures and a conjugation system, including E1 activating enzymes, E2 conjugation
enzymes, and E3 ligases, as well as proteases for deconjugation and recycling of the tags. However, each of the ULP posttranslational
modifications possesses its own specific enzymes and modifies its specific targets selectively. This review discusses recent
findings on ubiquitination and ubiquitin-like modifier processes and their roles in the posttranslational modification of
proteins in Arabidopsis. 相似文献
5.
6.
Hong Sung Hyun Singh Sudhir Tripathi Bhumi Nath Mondal Suvendu Lee Sangmin Jung Hyun Suk Cho Chuloh Kaur Shubhpreet Kim Jin-Hong Lee Sungbeom Bai Hyoung-Woo Bae Hyeun-Jong Lee Sang Yeol Lee Seung Sik Chung Byung Yeoup 《Protoplasma》2020,257(3):807-817
Protoplasma - Alkyl hydroperoxide reductase subunit F (AhpF) is a well-known flavoprotein that transfers electrons from pyridine nucleotides to the peroxidase protein AhpC via redox-active... 相似文献
7.
Pitna Kim Jin Hee Park Chang Soon Choi Inah Choi So Hyun Joo Min Kyoung Kim Soo Young Kim Ki Chan Kim Seung Hwa Park Kyoung Ja Kwon Jongmin Lee Seol-Heui Han Jong Hoon Ryu Jae Hoon Cheong Jung Yeol Han Ki Narm Ko Chan Young Shin 《Neurochemical research》2013,38(3):620-631
Prenatal exposure to alcohol has consistently been associated with adverse effects on neurodevelopment, which is collectively called fetal alcohol spectrum disorder (FASD). Increasing evidence suggest that prenatal exposure to alcohol increases the risk of developing attention deficit/hyperactivity disorder-like behavior in human. In this study, we investigated the behavioral effects of prenatal exposure to EtOH in offspring mice and rats focusing on hyperactivity and impulsivity. We also examined changes in dopamine transporter and MeCP2 expression, which may underlie as a key neurobiological and epigenetic determinant in FASD and hyperactive, inattentive and impulsive behaviors. Mouse or rat offspring born from dam exposed to alcohol during pregnancy (EtOH group) showed hyper locomotive activity, attention deficit and impulsivity. EtOH group also showed increased dopamine transporter and norepinephrine transporter level compared to control group in the prefrontal cortex and striatum. Prenatal exposure to EtOH also significantly decreased the expression of MeCP2 in both prefrontal cortex and striatum. These results suggest that prenatal exposure to EtOH induces hyperactive, inattentive and impulsive behaviors in rodent offspring that might be related to global epigenetic changes as well as aberration in catecholamine neurotransmitter transporter system. 相似文献
8.
Joon Ha Park Choong Hyun Lee In Hye Kim Ji Hyeon Ahn Jeong-Hwi Cho Bing Chun Yan Jae-Chul Lee Tae Hun Lee Jeong Yeol Seo Jun Hwi Cho Moo-Ho Won Il-Jun Kang 《Neurochemical research》2013,38(12):2640-2649
Glucose is a main energy source for normal brain functions. Glucokinase (GK) plays an important role in glucose metabolism as a glucose sensor, and GK activity is modulated by glucokinase regulatory protein (GKRP). In this study, we examined the changes of GK and GKRP immunoreactivities in the gerbil hippocampus after 5 min of transient global cerebral ischemia. In the sham-operated-group, GK and GKRP immunoreactivities were easily detected in the pyramidal neurons of the stratum pyramidale of the hippocampus. GK and GKRP immunoreactivities in the pyramidal neurons were distinctively decreased in the hippocampal CA1 region (CA), not CA2/3, 3 days after ischemia–reperfusion (I–R). Five days after I–R, GK and GKRP immunoreactivities were hardly detected in the CA1, not CA2/3, pyramidal neurons; however, at this point in time, GK and GKRP immunoreactivities were newly expressed in astrocytes, not microglia, in the ischemic CA1. In brief, GK and GKRP immunoreactivities are changed in pyramidal neurons and newly expressed in astrocytes in the ischemic CA1 after transient cerebral ischemia. These indicate that changes of GK and GKRP expression may be related to the ischemia-induced neuronal damage/death. 相似文献
9.
10.
Yong‐Chan Kwon In‐Seok Oh Nahum Lee Kyung‐Ho Lee Yeo Joon Yoon Eun Yeol Lee Byung‐Gee Kim Dong‐Myung Kim 《Biotechnology and bioengineering》2013,110(4):1193-1200
Harnessing the isolated protein synthesis machinery, cell‐free protein synthesis reproduces the cellular process of decoding genetic information in artificially controlled environments. More often than not, however, generation of functional proteins requires more than simple translation of genetic sequences. For instance, many of the industrially important enzymes require non‐protein prosthetic groups for biological activity. Herein, we report the complete cell‐free biogenesis of a heme prosthetic group and its integration with concurrent apoenzyme synthesis for the production of functional P450 monooxygenase. Step reactions required for the syntheses of apoenzyme and the prosthetic group have been designed so that these two separate pathways take place in the same reaction mixture, being insulated from each other. Combined pathways for the synthesis of functional P450 monooxygenase were then further integrated with in situ assay reactions to enable real‐time measurement of enzymatic activity during its synthesis. Biotechnol. Bioeng. 2013; 110: 1193–1200. © 2012 Wiley Periodicals, Inc. 相似文献