全文获取类型
收费全文 | 2976篇 |
免费 | 262篇 |
出版年
2023年 | 9篇 |
2022年 | 29篇 |
2021年 | 46篇 |
2020年 | 25篇 |
2019年 | 35篇 |
2018年 | 47篇 |
2017年 | 44篇 |
2016年 | 68篇 |
2015年 | 110篇 |
2014年 | 155篇 |
2013年 | 141篇 |
2012年 | 174篇 |
2011年 | 207篇 |
2010年 | 142篇 |
2009年 | 122篇 |
2008年 | 152篇 |
2007年 | 155篇 |
2006年 | 161篇 |
2005年 | 118篇 |
2004年 | 157篇 |
2003年 | 140篇 |
2002年 | 140篇 |
2001年 | 66篇 |
2000年 | 66篇 |
1999年 | 59篇 |
1998年 | 43篇 |
1997年 | 36篇 |
1996年 | 35篇 |
1995年 | 18篇 |
1994年 | 24篇 |
1993年 | 24篇 |
1992年 | 40篇 |
1991年 | 38篇 |
1990年 | 35篇 |
1989年 | 31篇 |
1988年 | 29篇 |
1987年 | 20篇 |
1986年 | 29篇 |
1985年 | 30篇 |
1984年 | 22篇 |
1983年 | 24篇 |
1982年 | 18篇 |
1981年 | 11篇 |
1980年 | 11篇 |
1979年 | 24篇 |
1978年 | 21篇 |
1977年 | 10篇 |
1976年 | 14篇 |
1972年 | 13篇 |
1971年 | 9篇 |
排序方式: 共有3238条查询结果,搜索用时 171 毫秒
141.
Direct measurement of oscillatory RhoA activity in embryonic cortical neurons stimulated with the axon guidance cue netrin‐1 using fluorescence resonance energy transfer
下载免费PDF全文
![点击此处可从《Biology of the cell / under the auspices of the European Cell Biology Organization》网站下载免费的PDF全文](/ch/ext_images/free.gif)
142.
Adrien Pinot Frédéric Barraquand Edoardo Tedesco Vincent Lecoustre Vincent Bretagnolle Bertrand Gauffre 《Population Ecology》2016,58(3):395-405
Common voles in western France exhibit three-year population cycles with winter crashes after large outbreaks. During the winter of 2011–2012, we monitored survival, reproduction, recruitment and population growth rate of common voles at different densities (from low to outbreak densities) in natura to better understand density dependence of demographic parameters. Between October and April, the number of animals decreased irrespective of initial density. However, the decline was more pronounced when October density was higher (loss of ≈54 % of individuals at low density and 95 % at high density). Using capture-mark-recapture models with Pradel's temporal symmetry approach, we found a negative effect of density on recruitment and reproduction. In contrast, density had a slightly positive effect on survival indicating that mortality did not drive the steeper declines in animal numbers at high density. We discuss these results in a population cycle framework, and suggest that crashes after outbreaks could reflect negative effects of density dependence on reproduction rather than changes in mortality rates. 相似文献
143.
Bertrand Brandt Munoz-Garay Carlos 《International journal of peptide research and therapeutics》2019,25(4):1441-1450
International Journal of Peptide Research and Therapeutics - Multidrug resistant pathogenic microorganisms have created a global health crisis. This resistance emerged due to the improper use of... 相似文献
144.
Thibault Teissier Valentine Quersin Viviane Gnemmi Mait Daroux Mike Howsam Florian Delguste Ccile Lemoine Chantal Fradin Ann‐Marie Schmidt Christelle Cauffiez Thierry Brousseau Franois Glowacki Frdric J. Tessier Eric Boulanger Marie Frimat 《Aging cell》2019,18(2)
Pro‐aging effects of endogenous advanced glycation end‐products (AGEs) have been reported, and there is increasing interest in the pro‐inflammatory and ‐fibrotic effects of their binding to RAGE (the main AGE receptor). The role of dietary AGEs in aging remains ill‐defined, but the predominantly renal accumulation of dietary carboxymethyllysine (CML) suggests the kidneys may be particularly affected. We studied the impact of RAGE invalidation and a CML‐enriched diet on renal aging. Two‐month‐old male, wild‐type (WT) and RAGE?/? C57Bl/6 mice were fed a control or a CML‐enriched diet (200 μg CML/gfood) for 18 months. Compared to controls, we observed higher CML levels in the kidneys of both CML WT and CML RAGE?/? mice, with a predominantly tubular localization. The CML‐rich diet had no significant impact on the studied renal parameters, whereby only a trend to worsening glomerular sclerosis was detected. Irrespective of diet, RAGE?/? mice were significantly protected against nephrosclerosis lesions (hyalinosis, tubular atrophy, fibrosis and glomerular sclerosis) and renal senile apolipoprotein A‐II (ApoA‐II) amyloidosis (p < 0.001). A positive linear correlation between sclerosis score and ApoA‐II amyloidosis score (r = 0.92) was observed. Compared with old WT mice, old RAGE?/? mice exhibited lower expression of inflammation markers and activation of AKT, and greater expression of Sod2 and SIRT1. Overall, nephrosclerosis lesions and senile amyloidosis were significantly reduced in RAGE?/? mice, indicating a protective effect of RAGE deletion with respect to renal aging. This could be due to reduced inflammation and oxidative stress in RAGE?/? mice, suggesting RAGE is an important receptor in so‐called inflamm‐aging. 相似文献
145.
Claire Bertrand Annabelle Thibessard Claude Bruand Franois Lecointe Pierre Leblond 《Molecular microbiology》2019,111(5):1139-1151
Double‐strand breaks (DSBs) are the most detrimental DNA damage encountered by bacterial cells. DBSs can be repaired by homologous recombination thanks to the availability of an intact DNA template or by Non‐Homologous End Joining (NHEJ) when no intact template is available. Bacterial NHEJ is performed by sets of proteins of growing complexity from Bacillus subtilis and Mycobacterium tuberculosis to Streptomyces and Sinorhizobium meliloti. Here, we discuss the contribution of these models to the understanding of the bacterial NHEJ repair mechanism as well as the involvement of NHEJ partners in other DNA repair pathways. The importance of NHEJ and of its complexity is discussed in the perspective of regulation through the biological cycle of the bacteria and in response to environmental stimuli. Finally, we consider the role of NHEJ in genome evolution, notably in horizontal gene transfer. 相似文献
146.
147.
Liwang Liu Laurent Plawinski Marie‐Christine Durrieu Bertrand Audoin 《Journal of biophotonics》2019,12(8)
Advances in microscopy with new visualization possibilities often bring dramatic progress to our understanding of the intriguing cellular machinery. Picosecond optoacoustic micro‐spectroscopy is an optical technique based on ultrafast pump‐probe generation and detection of hypersound on time durations of picoseconds and length scales of nanometers. It is experiencing a renaissance as a versatile imaging tool for cell biology research after a plethora of applications in solid‐state physics. In this emerging context, this work reports on a dual‐probe architecture to carry out real‐time parallel detection of the hypersound propagation inside a cell that is cultured on a metallic substrate, and of the hypersound reflection at the metal/cell adhesion interface. Using this optoacoustic modality, several biophysical properties of the cell can be measured in a noncontact and label‐free manner. Its abilities are demonstrated with the multiple imaging of a mitotic macrophage‐like cell in a single run experiment. 相似文献
148.
The main selective force driving floral evolution and diversity is plant–pollinator interactions. Pollinators use floral signals and indirect cues to assess flower reward, and the ensuing flower choice has major implications for plant fitness. While many pollinator behaviors have been described, the impact of parasites on pollinator foraging decisions and plant–pollinator interactions have been largely overlooked. Growing evidence of the transmission of parasites through the shared‐use of flowers by pollinators demonstrate the importance of behavioral immunity (altered behaviors that enhance parasite resistance) to pollinator health. During foraging bouts, pollinators can protect themselves against parasites through self‐medication, disease avoidance, and grooming. Recent studies have documented immune behaviors in foraging pollinators, as well as the impacts of such behaviors on flower visitation. Because pollinator parasites can affect flower choice and pollen dispersal, they may ultimately impact flower fitness. Here, we discuss how pollinator immune behaviors and floral traits may affect the presence and transmission of pollinator parasites, as well as how pollinator parasites, through these immune behaviors, can impact plant–pollinator interactions. We further discuss how pollinator immune behaviors can impact plant fitness, and how floral traits may adapt to optimize plant fitness in response to pollinator parasites. We propose future research directions to assess the role of pollinator parasites in plant–pollinator interactions and evolution, and we propose better integration of the role of pollinator parasites into research related to pollinator optimal foraging theory, floral diversity and agricultural practices. 相似文献
149.
150.
FXR-deficiency confers increased susceptibility to torpor 总被引:1,自引:0,他引:1
Cariou B Bouchaert E Abdelkarim M Dumont J Caron S Fruchart JC Burcelin R Kuipers F Staels B 《FEBS letters》2007,581(27):5191-5198
The role of the nuclear receptor FXR in adaptive thermogenesis was investigated using FXR-deficient mice. Despite elevated serum bile acid concentrations and increased mRNA expression profiles of thermogenic genes in brown adipose tissue, FXR-deficiency did not alter energy expenditure under basal conditions. However, FXR-deficiency accelerated the fasting-induced entry into torpor in a leptin-dependent manner. FXR-deficient mice were also extremely cold-intolerant. These altered responses may be linked to a more rapid decrease in plasma concentrations of metabolic fuels (glucose, triglycerides) thus impairing uncoupling protein 1-driven thermogenesis. These results identify FXR as a modulator of energy homeostasis. 相似文献