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391.
Adaptation and Acclimation of Photosynthetic Microorganisms to Permanently Cold Environments 总被引:5,自引:0,他引:5
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Rachael M. Morgan-Kiss John C. Priscu Tessa Pocock Loreta Gudynaite-Savitch Norman P. A. Huner 《Microbiological reviews》2006,70(1):222-252
Persistently cold environments constitute one of our world's largest ecosystems, and microorganisms dominate the biomass and metabolic activity in these extreme environments. The stress of low temperatures on life is exacerbated in organisms that rely on photoautrophic production of organic carbon and energy sources. Phototrophic organisms must coordinate temperature-independent reactions of light absorption and photochemistry with temperature-dependent processes of electron transport and utilization of energy sources through growth and metabolism. Despite this conundrum, phototrophic microorganisms thrive in all cold ecosystems described and (together with chemoautrophs) provide the base of autotrophic production in low-temperature food webs. Psychrophilic (organisms with a requirement for low growth temperatures) and psychrotolerant (organisms tolerant of low growth temperatures) photoautotrophs rely on low-temperature acclimative and adaptive strategies that have been described for other low-temperature-adapted heterotrophic organisms, such as cold-active proteins and maintenance of membrane fluidity. In addition, photoautrophic organisms possess other strategies to balance the absorption of light and the transduction of light energy to stored chemical energy products (NADPH and ATP) with downstream consumption of photosynthetically derived energy products at low temperatures. Lastly, differential adaptive and acclimative mechanisms exist in phototrophic microorganisms residing in low-temperature environments that are exposed to constant low-light environments versus high-light- and high-UV-exposed phototrophic assemblages. 相似文献
392.
Tessa Lloyd Pia Steigler Cheleka A. M. Mpande Virginie Rozot Boitumelo Mosito Constance Schreuder Timothy D. Reid Mark Hatherill Thomas J. Scriba Francesca Little Elisa Nemes the ACS Study Team 《PLoS computational biology》2021,17(7)
The risk of tuberculosis (TB) disease is higher in individuals with recent Mycobacterium tuberculosis (M.tb) infection compared to individuals with more remote, established infection. We aimed to define blood-based biomarkers to distinguish between recent and remote infection, which would allow targeting of recently infected individuals for preventive TB treatment. We hypothesized that integration of multiple immune measurements would outperform the diagnostic performance of a single biomarker. Analysis was performed on different components of the immune system, including adaptive and innate responses to mycobacteria, measured on recently and remotely M.tb infected adolescents. The datasets were standardized using variance stabilizing scaling and missing values were imputed using a multiple factor analysis-based approach. For data integration, we compared the performance of a Multiple Tuning Parameter Elastic Net (MTP-EN) to a standard EN model, which was built to the individual adaptive and innate datasets. Biomarkers with non-zero coefficients from the optimal single data EN models were then isolated to build logistic regression models. A decision tree and random forest model were used for statistical confirmation. We found no difference in the predictive performances of the optimal MTP-EN model and the EN model [average area under the receiver operating curve (AUROC) = 0.93]. EN models built to the integrated dataset and the adaptive dataset yielded identically high AUROC values (average AUROC = 0.91), while the innate data EN model performed poorly (average AUROC = 0.62). Results also indicated that integration of adaptive and innate biomarkers did not outperform the adaptive biomarkers alone (Likelihood Ratio Test χ2 = 6.09, p = 0.808). From a total of 193 variables, the level of HLA-DR on ESAT6/CFP10-specific Th1 cytokine-expressing CD4 cells was the strongest biomarker for recent M.tb infection. The discriminatory ability of this variable was confirmed in both tree-based models.A single biomarker measuring M.tb-specific T cell activation yielded excellent diagnostic potential to distinguish between recent and remote M.tb infection. 相似文献
393.
Use of SSCP analysis to identify germline mutations in HNPCC families fulfilling the Amsterdam criteria 总被引:14,自引:0,他引:14
N. E. Beck I. P. M. Tomlinson Tessa Homfray I. Frayling Shirley V. Hodgson Chris Harocopos Walter F. Bodmer 《Human genetics》1997,99(2):219-224
Hereditary non-polyposis colorectal cancer (HNPCC) is a clinical syndrome characterised by an inherited predisposition to
early onset colorectal and uterine cancers and an increased incidence of other cancers. It is caused by germline defects in
the human mismatch repair genes. Defects in two of the known mismatch repair genes (namely hMSH2 and hMLH1) account for over
90% of mutations found in HNPCC families. In this study we have identified 14 families that fulfilled the clinical criteria
for HNPCC and screened the hMSH2 and hMLH1 genes for germline mutations using single-strand conformational polymorphism (SSCP)
analysis and DNA sequencing. Seven mutations were identified. Of these, there were five frameshifts, one missense mutation
and a further novel mutation that involved separate transition and transversion changes in successive amino acid residues.
Three of the mutations were in hMSH2 and four in hMLH1. The identification of germ-line mutations in an HNPCC family enables
targeted surveillance and the possibility of early curative intervention. SSCP is a simple and effective method for identifying
most mutations in the human mismatch repair genes using DNA from fresh, frozen or archival material.
Received: 24 July 1996 / Revised: 26 September 1996 相似文献
394.
The cellular and molecular basis of peripheral nerve regeneration 总被引:48,自引:0,他引:48
Functional recovery from peripheral nerve injury and repair depends on a multitude of factors, both intrinsic and extrinsic
to neurons. Neuronal survival after axotomy is a prerequisite for regeneration and is facilitated by an array of trophic factors
from multiple sources, including neurotrophins, neuropoietic cytokines, insulin-like growth factors (IGFs), and glial-cell-line-derived
neurotrophic factors (GDNFs). Axotomized neurons must switch from a transmitting mode to a growth mode and express growth-associated
proteins, such as GAP-43, tubulin, and actin, as well as an array of novel neuropeptides and cytokines, all of which have
the potential to promote axonal regeneration. Axonal sprouts must reach the distal nerve stump at a time when its growth support
is optimal. Schwann cells in the distal stump undergo proliferation and phenotypical changes to prepare the local environment
to be favorable for axonal regeneration. Schwann cells play an indispensable role in promoting regeneration by increasing
their synthesis of surface cell adhesion molecules (CAMs), such asN-CAM, Ng-CAM/L1, N-cadherin, and L2/HNK-1, by elaborating basement membrane that contains many extracellular matrix proteins,
such as laminin, fibronectin, and tenascin, and by producing many neurotrophic factors and their receptors. However, the growth
support provided by the distal nerve stump and the capacity of the axotomized neurons to regenerate axons may not be sustained
indefinitely. Axonal regeneration may be facilitated by new strategies that enhance the growth potential of neurons and optimize
the growth support of the distal nerve stump in combination with prompt nerve repair. 相似文献
395.
Molinacuaria indonesiensis n. sp. from the stomach of Rattus argentiventer, the ricefield rat, in Sukamandi, Java, Indonesia is described and figured from the examination of seven males and three
females. The species is separated from the three other species of the genus, namely, M. bendelli (Adams & Gibson, 1969) Wong & Lankester, 1985, M. acholonui (Schmidt & Kuntz, 1972) Wong & Lankester, 1985 and M. gallinulae (Wang, 1966) Wong & Lankester, 1985. The species is characterised by (i) the larger body measurements, (ii) the presence
of a fold rather than a deep groove separating the anterior cephalic region and the ptilina which form four distinct shields,
(iii) the morphology of the four ptilina, each one being tripartite with a pointed middle section twice as long at the two
lateral sections (iv) the number of papillae on the posterior end of the male (two pairs pre-cloacal, four pairs post-cloacal),
(v) the morphology of the left spicule, and (vi) the female tail being straight with a terminal knob. 相似文献
396.
397.
Arne Janssen Farid Faraji Tessa van der Hammen Sara Magalhães & Maurice W. Sabelis 《Ecology letters》2002,5(4):490-494
It is well known that young, small predator stages are vulnerable to predation by conspecifics, intra-guild competitors or hyperpredators. It is less known that prey can also kill vulnerable predator stages that present no danger to the prey. Since adult predators are expected to avoid places where their offspring would run a high predation risk, this opens the way for potential prey to deter dangerous predator stages by killing vulnerable predator stages. We present an example of such a complex predator–prey interaction. We show that (1) the vulnerable stage of an omnivorous arthropod prey discriminates between eggs of a harmless predator species and eggs of a dangerous species, killing more eggs of the latter; (2) prey suffer a minor predation risk from newly hatched predators; (3) adult predators avoid ovipositing near killed predator eggs, and (4) vulnerable prey near killed predator eggs experience an almost fourfold reduction of predation. Hence, by attacking the vulnerable stage of their predator, prey deter adult predators and thus reduce their own predation risk. This provides a novel explanation for the killing of vulnerable stages of predators by prey and adds a new dimension to anti-predator behaviour. 相似文献
398.
Tessa Goetghebuer Martin O C Ota Bunja Kebbeh Malick John Dolly Jackson-Sillah Johan Vekemans Arnaud Marchant Melanie Newport Helen A Weiss 《Twin research》2003,6(4):279-284
Twins are prone to developmental delay due to prematurity and low birthweight. However it is unknown if twinning is an independent risk factor for developmental delay. The objective of this study was to compare the attainment of a set of gross motor milestones in a cohort of twins and singletons in The Gambia. Eighty-four pairs of twins and 72 singletons were enrolled at birth and followed up until 18 months of age. The mean age at achieving milestones was higher in twins for each development outcome and the difference between twins and singletons was significant after adjustment for confounders for maintaining head, sitting without support and walking. In twins, we found a highly significant correlation within pairs for most milestones. When monozygotic and dizygotic twins were compared, a significant heritability was observed for crawling, sitting, standing and walking, with over 90% of population variance observed due to genetic factors rather than environmental factors. There was little evidence for a genetic contribution towards very early milestones. In conclusion, our data suggest that twinning is an independent risk factor for developmental delay in early life in The Gambia, and that genetic factors contribute strongly to certain motor development outcomes. 相似文献
399.
Several studies have found relationships between early life factors (birth weight, length of gestation, height, weight, duration of breast-feeding, maternal age, social class, periods of infection, presence of adverse life events, and quality of housing conditions in childhood) and age at menarche but none has considered all of these factors in the same study. The follow-up study of the Newcastle Thousand Families birth cohort, established in 1947, provided age at menarche data collected retrospectively at age 50 from 276 women who returned self-completion questionnaires in 1997. Three main independent associations were observed: girls who experienced a shorter gestation, girls whose mothers were younger when they were born, and girls who were heavier at age 9 had earlier menarche. Birth weight, standardized for gestational age, was found to have different relationships with age at menarche depending upon how heavy or light a girl was at age 9. The results of this study support the hypotheses that conditions in fetal and early life are associated with the timing of menarche and that greater childhood growth is associated with earlier menarche. It is suggested that future work should focus on illuminating the mechanisms underlying these statistical relationships. 相似文献
400.
Tessa H. Pocock Vaughan Hurry Leonid V. Savitch Norman P. A. Huner 《Physiologia plantarum》2001,113(4):499-506
Five winter and five spring wheat ( Triticum aestivum L.) cultivars were grown under either control conditions (20°C/250 photosynthetic photon flux density (PPFD) [μmol m−2 s−1 ]), high irradiance (20°C/800 PPFD) or at low temperature (either 5°C/250 PPFD or 5°C/50 PPFD). To eliminate any potential bias, the wheat cultivars were arbitrarily chosen without any previous knowledge of their freezing tolerance or photosynthetic competence. We show that the differential susceptibilities to photoinhibition exhibited between spring and winter wheat cultivars, as assessed by chlorophyll fluorescence cannot be explained on the basis of either growth irradiance or low growth temperature per se. The role of excitation pressure is discussed. We assessed the correlation between susceptibility to low-temperature photoinhibition, maximum ribulose 1,5-bisphosphate carboxylase-oxygenase (EC 4.1.1.39) and NADP-dependent malate dehydrogenase (EC 1.1.1.82) activities, chlorophyll and protein concentrations and freezing tolerance determined by electrolyte leakage. Susceptibility to photoinhibition is the only parameter examined that is strongly and negatively correlated with freezing tolerance. We suggest that the assessment of susceptibility to photoinhibition may be a useful predictor of freezing tolerance and field survival of cereals. 相似文献