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排序方式: 共有186条查询结果,搜索用时 15 毫秒
171.
ABSTRACT: BACKGROUND: When genetic structure is identified using mitochondrial DNA (mtDNA), but no structure is identified using biparentally-inherited nuclear DNA, the discordance is often attributed to differences in dispersal potential between the sexes. RESULTS: We sampled the intertidal rocky shore mussel Perna perna in a South African bay and along the nearby open coast, and sequenced maternally-inherited mtDNA (there is no evidence for paternally-inherited mtDNA in this species) and a biparentally-inherited marker. By treating males and females as different populations, we identified significant genetic structure on the basis of mtDNA data in the females only. CONCLUSIONS: This is the first study to report sex-specific differences in genetic structure based on matrilineally-inherited mtDNA in a passively dispersing species that lacks social structure or sexual dimorphism. The observed pattern most likely stems from females being more vulnerable to selection in habitats from which they did not originate, which also manifests itself in a male-biased sex ratio. Our results have three important implications for the interpretation of population genetic data. First, even when mtDNA is inherited exclusively in the female line, it also contains information about males. For that reason, using it to identify sex-specific differences in genetic structure by contrasting it with biparentally-inherited markers is problematic. Second, the fact that sex-specific differences were found in a passively dispersing species in which sex-biased dispersal is unlikely highlights the fact that significant genetic structure is not necessarily a function of low dispersal potential or physical barriers. Third, even though mtDNA is typically used to study historical demographic processes, it also contains information about contemporary processes. Higher survival rates of males in non-native habitats can erase the genetic structure present in their mothers within a single generation. 相似文献
172.
Jörg Teske Jens-Peter WellerArmin Fieguth Thomas RothämelYvonne Schulz Hans Dieter Tröger 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2010,878(27):2659-2663
The current paper describes a validated method for the detection and quantification of naphthalen-1-yl-(1-pentylindol-3-yl)methanone (JWH-018), an ingredient of a herbal mixture called “Spice”, by means of HPLC–ESI–MS–MS in serum. Lower limit of detection and lower limit of quantification were 0.07 and 0.21 ng/ml, respectively. In 2 subjects who consumed ca. 50 μg/kg of JWH-018 by smoking, the active ingredient was detected by means of the described method. Thereby, the serum concentrations reached values of approx. 10 ng/ml and dropped within 3 h very fast (<10% of the measured maximum concentrations). 相似文献
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175.
Teske CA von Lieres E Schröder M Ladiwala A Cramer SM Hubbuch JJ 《Biotechnology and bioengineering》2006,95(1):58-66
Confocal laser scanning microscopy has been previously applied to the study of protein uptake in porous chromatography resins. This method requires labeling the protein with a fluorescent probe. The labeled protein is then diluted with a large quantity of native protein so that the fluorescence intensity is a linear function of the labeled protein concentration. Ideally, the attachment of a fluorescent probe should not affect the affinity of the protein for the stationary phase; however, recent experimental work has shown that this assumption is difficult to satisfy. In the present study, we present a mathematical model of protein diffusion and adsorption in a single adsorbent particle. The differences in adsorption behavior of labeled and native protein are accounted for by treating the system as a two-component system (labeled and native protein) described by the steric mass action isotherm (SMA). SMA parameters are regressed from experimental linear gradient elution data for lysozyme and lysozyme-dye conjugates (for the fluorescent dyes Cy3, Cy5, Bodipy FL, and Atto635). When the regressed parameters are employed in the model, an overshoot in the labeled lysozyme concentration is predicted for Cy5- and Bodipy-labeled lysozyme, but not for Atto635-labeled lysozyme. The model predictions agree qualitatively well with recent work showing the dependence of the concentration overshoot on the identity of the attached dye and provide further evidence that the overshoot is likely caused by the change of binding characteristics due to the fluorescent label. 相似文献
176.
Sondra S. Teske Robert G. Arnold 《Reviews in Environmental Science and Biotechnology》2008,7(2):107-124
The ecological impacts of natural estrogens and xenoestrogens in treated wastewater include altered sexual development and
sex ratios among continuously exposed organisms. The primary sources of estrogenic activity in wastewater are natural estrogens
such as estrone, 17β-estradiol and estriol and synthetic compounds like 17α-ethinylestradiol, alkylphenols and alklphenol
ethoxylates. Precursors in raw wastewater can yield estrogenic intermediates during wastewater treatment. All these compounds
can be destroyed by biochemical processes, albeit at significantly different rates or under different conditions. That is,
estrogenic compounds can be, but are not always, destroyed by conventional wastewater treatment processes, suggesting that
conventional processes can be optimized for removal of estrogenic activity from wastewater. Sorption to sludges derived from
wastewater treatment affects the fates of hydrophobic xenoestrogens such as nonylphenol, in part because the biodegradability
of sorbed contaminants is limited. It may also be possible to tailor sludge stabilization processes to remove trace contaminants,
including estrogens. For example, there are significant differences in the efficiencies of aerobic and anaerobic digestion
for destruction of alkylphenols and probably other estrogenic compounds with aromatic moieties. Because advanced wastewater
treatment is not economically feasible for most communities, there is ample incentive to develop accurate relationships between
operational parameters and removal of estrogenic compounds during secondary wastewater treatment. 相似文献
177.
Bahr M Crump BC Klepac-Ceraj V Teske A Sogin ML Hobbie JE 《Environmental microbiology》2005,7(8):1175-1185
Sulfate reduction, mediated by sulfate-reducing bacteria (SRB), is the dominant remineralization pathway in sediments of New England salt marshes. High sulfate reduction rates are associated with the rhizosphere of Spartina alterniflora when plants elongate aboveground. The growth process concurrently produces significant amounts of new rhizome material belowground and the plants leak dissolved organic compounds. This study investigated the diversity of SRB in a salt marsh over an annual growth cycle of S. alterniflora by exploring the diversity of a functional gene, dissimilatory sulfite reductase (dsrAB). Because the dsrAB gene is a key gene in the anaerobic sulfate-respiration pathway, it allows the identification of microorganisms responsible for sulfate reduction. Conserved dsrAB primers in polymerase chain reaction (PCR) generated full-length dsrAB amplicons for cloning and DNA sequence analysis. Nearly 80% of 380 clone sequences were similar to genes from Desulfosarcina and Desulfobacterium species within Desulfobacteraceae. This reinforces the hypothesis that complete oxidizers with high substrate versatility dominate the marsh. However, the phylotypes formed several clades that were distinct from cultured representatives, indicating a greater diversity of SRB than previously appreciated. Several dsrAB sequences were related to homologues from gram-positive, thermophilic and non-thermophilic Desulfotomaculum species. One dsrAB lineage formed a sister group to cultured members of the delta-proteobacterial group Syntrophobacteraceae. A deeply branching dsrAB lineage was not affiliated with genes from any cultured SRB. The sequence data from this study will allow for the design of probes or primers that can quantitatively assess the diverse range of sulfate reducers present in the environment. 相似文献
178.
179.
Kamphuis J. A. M. Linschoten M. Cramer M. J. Alsemgeest F. van Kessel D. J. W. Urgel K. Post M. C. Manintveld O. C. Hassing H. C. Liesting C. Wardeh A. J. Olde Bijvank E. G. M. Schaap J. Stevense-den Boer A. M. Doevendans P. A. Asselbergs F. W. Teske A. J. 《Netherlands heart journal》2021,29(5):288-294
Netherlands Heart Journal - The relative new subspecialty ‘cardio-oncology’ was established to meet the growing demand for an interdisciplinary approach to the management of cancer... 相似文献
180.
Anne Ammerdorffer Hendrik-I J. Roest Annemieke Dinkla Jacob Post Teske Schoffelen Marcel van Deuren Tom Sprong Johanna M. Rebel 《PloS one》2014,9(10)
In humans, infection with Coxiella burnetii, the causative agent of Q fever, leads to acute or chronic infection, both associated with specific clinical symptoms. In contrast, no symptoms are observed in goats during C. burnetii infection, although infection of the placenta eventually leads to premature delivery, stillbirth and abortion. It is unknown whether these differences in clinical outcome are due to the early immune responses of the goats. Therefore, peripheral blood mononuclear cells (PBMCs) were isolated from pregnant goats. In total, 17 goats were included in the study. Six goats remained naive, while eleven goats were infected with C. burnetii. Toll-like receptor (TLR) and cytokine mRNA expression were measured after in vitro stimulation with heat-killed C. burnetii at different time points (prior infection, day 7, 35 and 56 after infection). In naive goats an increased expression of interleukin (IL)-1β, tumor necrosis factor (TNF)-α, IL-10 and interferon (IFN)-γ mRNA upon C. burnetii stimulation was detected. In addition, TLR2 expression was strongly up-regulated. In goats infected with C. burnetii, PBMCs re-stimulated in vitro with C. burnetii, expressed significantly more TNF-α mRNA and IFN-γ mRNA compared to naive goats. In contrast, IL-10 mRNA production capacity was down-regulated during C. burnetii infection. Interestingly, at day 7 after inoculation a decreased IFN-γ protein level was observed in stimulated leukocytes in whole blood from infected goats, whereas at other time-points increased production of IFN-γ protein was seen. Our study shows that goats initiate a robust pro-inflammatory immune response against C. burnetii in vitro. Furthermore, PBMCs from C. burnetii infected goats show augmented pro-inflammatory cytokine responses compared to PBMCs from non-infected goats. However, despite this pro-inflammatory response, goats are not capable of clearing the C. burnetii infection. 相似文献