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91.
Masafumi Komiya Shigehiro Asano Nobuyuki Koike Erina Koga Junetsu Igarashi Shogo Nakatani Yoshiaki Isobe 《Bioorganic & medicinal chemistry》2012,20(23):6840-6847
Based on 2-(4-phenoxybenzoyl)-5-hydroxyindole (2), a novel structural class of CaMKII inhibitors were synthesized and further optimized. The strong acidity of the hydroxyl group and the lipophilic group at the 4 and 6-positions were found to be necessary for strong CaMKII inhibition. Compound 25 was identified as a promising compound with 50-fold more potent inhibitory activity for CaMKII than 2. Compound 25 also showed high selectivity for CaMKII over off-target kinases. 相似文献
92.
par Masanao Matsui Yôichi Nakatani Yasuki Mori et Zirô Nikuni 《Bioscience, biotechnology, and biochemistry》2013,77(9):647-649
The synthesis of 3-hydroxymethyl-4,5,7-trimethoxy-2-naphthoic acid lactone (II) is described. 相似文献
93.
Kota Nakatani Yuta Katano Kenji Kojima Teisuke Takita Rie Yatsunami Satoshi Nakamura 《Bioscience, biotechnology, and biochemistry》2018,82(10):1715-1723
Site saturation mutagenesis library is a recently developed technique, in which any one out of all amino acid residues in a target region is substituted into other 19 amino acid residues. In this study, we used this technique to increase the thermostability of a GH10 xylanase, XynR, from Bacillus sp. strain TAR-1. We hypothesized that the substrate binding region of XynR is flexible, and that the thermostability of XynR will increase if the flexibility of the substrate binding region is decreased without impairing the substrate binding ability. Site saturation mutagenesis libraries of amino acid residues Tyr43–Lys115 and Ala300–Asn325 of XynR were constructed. By screening 480 clones, S92E was selected as the most thermostable one, exhibiting the residual activity of 80% after heat treatment at 80°C for 15 min in the hydrolysis of Remazol Brilliant Blue-xylan. Our results suggest that this strategy is effective for stabilization of GH10 xylanase.
Abbreviations: DNS: 3,5-dinitrosalicylic acid; RBB-xylan: Remazol Brilliant Blue-xylan 相似文献
94.
A new limonoid, 7-acetyltrichilin A, has been isolated from the root bark of Trichilia roka and identified as an antifeedant against North American and Japanese pest insects. 相似文献
95.
A kinetic study of saccharopine dehydrogenase reaction 总被引:3,自引:0,他引:3
96.
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98.
Nakatani Y Hotta S Utsunomiya I Tanaka K Hoshi K Ariga T Yu RK Miyatake T Taguchi K 《Neurochemical research》2009,34(1):149-157
To investigate the pathophysiological mechanisms of immune-mediated peripheral neuropathies, we studied the effects of sera
from patients with Guillain-Barré syndrome (GBS) on the Cav2.1 voltage-dependent calcium channel (VDCC) current in Purkinje
cells. Using the whole-cell recording technique, Cav2.1 VDCC current was measured in cerebellar Purkinje cells in the presence
of serum from GBS patients with acute motor axonal neuropathy (AMAN) or acute inflammatory demyelinating polyneuropathy (AIDP).
The AMAN patient sera significantly inhibited the Cav2.1 VDCC current compared with healthy volunteer sera, and this inhibition
was fully reversible by washing out the AMAN serum. Similarly, IgG purified from AMAN sera also inhibited the Cav2.1 VDCC
current. However, the activation and inactivation kinetics of the Cav2.1 VDCC currents were not affected by serum from an
AMAN patient. Moreover, the VDCC current of Purkinje cells was also inhibited by IgG anti-GM1 monoclonal antibody (anti-GM1
mAb). In an immunocytochemical study using double fluorescence staining, Purkinje cells were stained by monoclonal IgG anti-GM1
mAb. In contrast, AIDP patient and healthy volunteer sera did not affect the Cav2.1 VDCC current. These results suggest that
in some case of GBS, particularly of AMAN patients with IgG anti-GM1 mAb, muscle weakness may be induced by dysfunction of
Cav2.1 VDCC functioning at the motor nerve terminals.
Special issue article in honor of Dr. George DeVries. 相似文献
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100.
Nakatani K Horie S Goto Y Kobori A Hagihara S 《Bioorganic & medicinal chemistry》2006,14(15):5384-5388
Drugs targeting the stem-loop IIB of Rev responsible element (RRE) of HIV-1 mRNA are potential therapeutic agents for HIV-1 infection. The stem loop is characterized by an internal loop consist of consecutive G-G and G-A mismatches, which is the single binding site for Rev protein for nuclear export of viral mRNA. We report here that ligands binding to G-G and G-A mismatches in duplex DNA also bind to the internal loop in competition with Rev peptide and lead to the dissociation of pre-formed Rev-RRE complex in a model system. 相似文献