全文获取类型
收费全文 | 575篇 |
免费 | 49篇 |
出版年
2021年 | 5篇 |
2019年 | 6篇 |
2018年 | 7篇 |
2017年 | 8篇 |
2016年 | 8篇 |
2015年 | 9篇 |
2014年 | 15篇 |
2013年 | 56篇 |
2012年 | 23篇 |
2011年 | 21篇 |
2010年 | 22篇 |
2009年 | 16篇 |
2008年 | 25篇 |
2007年 | 24篇 |
2006年 | 24篇 |
2005年 | 28篇 |
2004年 | 36篇 |
2003年 | 20篇 |
2002年 | 22篇 |
2001年 | 17篇 |
2000年 | 26篇 |
1999年 | 23篇 |
1998年 | 11篇 |
1997年 | 8篇 |
1996年 | 8篇 |
1995年 | 7篇 |
1994年 | 4篇 |
1993年 | 8篇 |
1992年 | 7篇 |
1991年 | 11篇 |
1990年 | 7篇 |
1989年 | 2篇 |
1988年 | 6篇 |
1987年 | 6篇 |
1985年 | 21篇 |
1984年 | 6篇 |
1983年 | 7篇 |
1982年 | 2篇 |
1980年 | 4篇 |
1979年 | 2篇 |
1978年 | 7篇 |
1977年 | 7篇 |
1976年 | 7篇 |
1975年 | 7篇 |
1974年 | 4篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1970年 | 4篇 |
1969年 | 3篇 |
1968年 | 3篇 |
排序方式: 共有624条查询结果,搜索用时 46 毫秒
31.
Keisuke Nakatani Haruto Ishikawa Shigetoshi Aono Yasuhisa Mizutani 《Biochemical and biophysical research communications》2013
The heme detoxification protein of the malaria parasite Plasmodium falciparum is involved in the formation of hemozoin, an insoluble crystalline form of heme. Although the disruption of hemozoin formation is the most widely used strategy for controlling the malaria parasite, the heme-binding properties of heme detoxification protein are poorly characterized. In this study, we established a method for the expression and purification of the non-tagged protein and characterized heme-binding properties. The spectroscopic features of non-tagged protein differ from those of the His-tagged protein, suggesting that the artificial tag interferes with the properties of the recombinant protein. The purified recombinant non-tagged heme detoxification protein had two heme-binding sites and exhibited a spectrum typical of heme proteins. A mechanism for hemozoin formation is proposed. 相似文献
32.
Masao Fujimaki Makoto Tajima Teruyo Matsumoto 《Bioscience, biotechnology, and biochemistry》2013,77(10):1228-1231
The free amino acids of potatoes irradiated with the doses of 7,000, 15,000 and 30,000 rad were determined by ion-exchange chromatography.After 15 days storage following irradiation, it was shown that the concentration of asparatic acid, proline and aliphatic amino acids increased with increasing irradiation doses, while that of basic amino acids and glutamic acid especially decreased. However, after 105 days of storage, the similarity of the free amino acid content of irradiated potatoes to that of non-irradiated and non-stored potatoes was observed.On the concentration of protein-bound amino acids, there were no significant differences between non-irradiated and 15,000 rad irradiated potatoes. 相似文献
33.
Masakazu Miyakado Isamu Nakayama Hirosuke Yoshioka Nobuji Nakatani 《Bioscience, biotechnology, and biochemistry》2013,77(7):1609-1611
It was evidenced that mutagenic principles in tryptophan pyrolysate, 3-amino-1,4-dimethyl-5H pyrido(4,3-b) indole and 3-amino-1-methyl-5H pyrido(4,3-b) indole (abbreviated as Trp-P-1 and Trp-P-2, respectively) bind to DNA without activation by rat liver microsomes. The bindings of Trp-P-1 and Trp-P-2 were not random and did not introduce strand scissions into DNA. Trp-P-1 bound more easily than Trp-P-2. The bindings of these mutagenic principles to DNA were concluded by using negatively superhelical simian virus 40 (SV40) DNA from following experimental data. (1) The intensity of ethidium bromide (EtBr)-DNA fluorescence by illumination with UV light and the electrophoretic mobility of superhelical DNA in agarose gel decreased as a function of the amounts of Trp-P-1 and Trp-P-2. (2) In vitro RNA synthesis catalyzed by Escherichia coli DNA-dependent RNA polymerase and nick-translation catalyzed by Escherichia coli DNA polymerase I (Kornberg enzyme) were inhibited significantly on DNA treated with Trp-P-1 and Trp-P-2. (3) The negative superhelicity of SV40 DNA introduces unpaired regions into DNA. These regions can be cleaved by single-strand-specific S1 endonuclease to generate unit length linear duplex molecules. It was found that this S1-sensitivity of DNA decreased by treatment with Trp-P-1. (4) The cleavage patterns of Trp-P-1 treated DNA with five restriction endonucleases were investigated. The protection of the cleavage site by the drug was observed against HincII, HindIII and EcoRII, whereas not against HaeIII and HinfI. These results show that the binding of Trp-P-1 to DNA is not random. Identical results were also obtained in Trp-P-2.However, the bindings of Trp-P-1 and Trp-P-2 were not so tight, and phenol extraction of the complex dissociated these drugs from DNA. 相似文献
34.
A new antioxidative glucoside (1a) was isolated from the leaves of oregano (Origanum vulgare L.), one of the herbal spices belonging to the Family Labiatae. The structure of 1a was determined as 4-(3,4-dihydroxybenzoyloxymethyl)phenyl-β-D-glucopyranoside, on the basis of chemical and spectroscopic evidence. In addition, the structure was confirmed by synthesizing its acetyl derivative (1b). 相似文献
35.
Michiko Nose Yoichi Nakatani Tei Yamanishi 《Bioscience, biotechnology, and biochemistry》2013,77(2):261-271
By continuing flavor analysis of green tea from a previous paper, further twenty seven compounds were newly identified. These compounds are limonene, α-cubebene, α-copaene, caryophyllene, α-humulene, α- and γ-muurolene, β-sesquiphellandrene, δ-cadinene, calamenene, cubenol, α-cadinol, α-terpineol, n-heptanol, n-nonanol, furfurylalcohol, n-nonanal, N-ethylformylpyrrole, pyrrylmethylketone, 6-methyl-trans-3,5-heptadien-2-one, 2′,2″-dihydro-α-ionone, 6,10,14-trimethyl-2-pentadecanone, cis-3-hexenylcaproate, cis-3-hexenylbenzoate, α-terpinylacetate, coumarin and diphenylamine.Relative quantities of known compounds in intermediate- and high-boiling fraction were determined. 相似文献
36.
Nobuji Nakatani Reiko Inatani Hidetsugu Fuwa 《Bioscience, biotechnology, and biochemistry》2013,77(12):2831-2836
Two phenolic amides were isolated from the fruits of white pepper (Piper nigrum L.) and identified to be N-trans-feruloyl tyramine (2a) and N-5-(4-hydroxyphenyl)-2E, 4E-pentadienoyl piperidine (6a) on the basis of chemical and specrtal evidence. Both compounds were synthesized. 相似文献
37.
Kenji Kobata Ikue Takemura Gaku Tago Takayuki Moriya Kaori Kubota Sachie Nakatani 《Bioscience, biotechnology, and biochemistry》2013,77(7):1242-1245
Long-chain N-vanillyl-acylamides (LCNVAs) were generated from plant oils and vanillylamine (VA) by nucleophilic amidation without any catalytic reagents. The resulting LCNVAs varied according to the fatty acid composition of the plant oil used. Therefore, the LCNVAs contained in Capsicum oleoresins were products that were spontaneously generated from the oleoresin during storage. 相似文献
38.
39.
Flesh‐eating Streptococcus pyogenes triggers the expression of receptor activator of nuclear factor‐κB ligand
下载免费PDF全文
![点击此处可从《Cellular microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Hidenori Matsui Yuriko Nakatani Haruno Yoshida Asako Takizawa Osamu Takeuchi Anders Øverby Takashi Takahashi Somay Y. Murayama Koichi Matsuo 《Cellular microbiology》2016,18(10):1390-1404
Human CD46 is a receptor for the M protein of group A streptococcus (GAS). The emm1 GAS strain GAS472 was isolated from a patient suffering from streptococcal toxic shock‐like syndrome. Human CD46‐expressing transgenic (Tg) mice developed necrotizing fasciitis associated with osteoclast‐mediated progressive and severe bone destruction in the hind paws 3 days after subcutaneous infection with 5 × 105 colony‐forming units of GAS472. GAS472 infection induced expression of the receptor activator of nuclear factor‐κB ligand (RANKL) while concomitantly reducing osteoprotegerin expression in the hind limb bones of CD46 Tg mice. Micro‐computed tomography analysis of the bones suggested that GAS472 infection induced local bone erosion and systemic bone loss in CD46 Tg mice. Because treatment with monoclonal antibodies (mAbs) against mouse CD4+ and CD8+ T lymphocytes did not inhibit osteoclastogenesis, T lymphocyte‐derived RANKL was not considered a major contributor to massive bone loss during GAS472 infection. However, immunohistochemical analysis of the hind limb bones showed that GAS472 infection stimulated RANKL production in various bone marrow cells, including fibroblast‐like cells. Treatment with a mAb against mouse RANKL significantly inhibited osteoclast formation and bone resorption. These data suggest that increased expression of RANKL in heterogeneous bone marrow cells provoked bone destruction during GAS infection. 相似文献
40.
Murakami Shinsuke Nakatani Jun Nakajima Kenichi Amasawa Eri Ii Ryota Hayashi Kiyotada Yoshikawa Naoki Daigo Ichiro Kishita Yusuke Ihara Tomohiko Shobatake Koichi Kudoh Yuki Motoshita Masaharu Kanemoto Keiichiro Hara Minako Kashiwagi Aiichiro Hashimoto Seiji Shigetomi Yosuke Kanzaki Masayuki Kikuchi Yasunori Ohno Hajime Fukushima Yasuhiro 《The International Journal of Life Cycle Assessment》2019,24(8):1544-1552
The International Journal of Life Cycle Assessment - 相似文献