全文获取类型
收费全文 | 2830篇 |
免费 | 202篇 |
国内免费 | 1篇 |
出版年
2021年 | 26篇 |
2019年 | 19篇 |
2018年 | 30篇 |
2017年 | 19篇 |
2016年 | 40篇 |
2015年 | 53篇 |
2014年 | 73篇 |
2013年 | 173篇 |
2012年 | 108篇 |
2011年 | 126篇 |
2010年 | 75篇 |
2009年 | 72篇 |
2008年 | 131篇 |
2007年 | 137篇 |
2006年 | 144篇 |
2005年 | 134篇 |
2004年 | 154篇 |
2003年 | 136篇 |
2002年 | 149篇 |
2001年 | 116篇 |
2000年 | 140篇 |
1999年 | 101篇 |
1998年 | 35篇 |
1997年 | 36篇 |
1996年 | 32篇 |
1995年 | 24篇 |
1994年 | 23篇 |
1993年 | 34篇 |
1992年 | 63篇 |
1991年 | 45篇 |
1990年 | 52篇 |
1989年 | 31篇 |
1988年 | 48篇 |
1987年 | 35篇 |
1986年 | 33篇 |
1985年 | 28篇 |
1984年 | 26篇 |
1983年 | 16篇 |
1982年 | 18篇 |
1981年 | 17篇 |
1980年 | 16篇 |
1979年 | 22篇 |
1978年 | 19篇 |
1974年 | 16篇 |
1973年 | 23篇 |
1972年 | 14篇 |
1971年 | 15篇 |
1970年 | 23篇 |
1969年 | 19篇 |
1966年 | 16篇 |
排序方式: 共有3033条查询结果,搜索用时 15 毫秒
61.
62.
63.
N. Ito K. Nishi M. Nakajima Y. Okamura T. Hirota 《Histochemistry and cell biology》1989,92(4):307-312
Summary Histochemical analyses of the chemical structures of sugar sequences with or without blood group specificity were carried out by combined stepwise digestion of tissue sections with exo-and endoglycosidases and subsequent lectin stainings in formalin-fixed, paraffin-embedded human pancreas. In acinar cells from blood group A or AB secretor individuals, sequential digestion with -N-acetylgalactosaminidase and -L-fucosidase imparted reactivity with peanut agglutinin (PNA) in cells reactive with Dolichos biflorus agglutinin as well as those with Ulex europaeus agglutinin I(UEA-I). Simple fucosidase digestion imparted the PNA reactivity only in UEA-I reactive cells. Sequential digestion with -galactosidase and fucosidase likewise liberated the PNA binding sites in Griffonia simplicifolia agglutinin I-B4 reactive cells from blood group B and AB secretors. Sialidase digestion liberated the PNA binding sites not only in acinar cells but also intercalated duct cells, islet cells of Langerhans and endothelial cells. The PNA reactivity obtained by these enzyme digestions was eliminted by endo--N-acetylgalactosaminidase (endo-GalNAcdase) digestion. Preexisting PNA affinity in acinar cells from nonsecretors was also susceptible to endo-GalNAcdase treatment. Following the endo-GalNAcdase digestion, fucosidase or sialidase digestion recovered the PNA reactivity in acinar cells from nonsecretors. These results show that ABH determinants carried on O-glycosidically linked type 3 chain (D-galactose-(1-3)-N-acetyl-D-galactosamine1-serine or threonine) are secreted in pancreatic acinar cells and suggest that product coded by the secretor gene is required for the complete conversion of type 3 precursor chains into H determinants. 相似文献
64.
A case of tracheal reconstruction with a palatal mucoperiosteal graft is reported. The patient is a 75-year-old woman who had the anterior wall of her trachea resected for invading thyroid carcinoma. Palatal mucoperiosteum, which is easy to handle, provided both the lining and supporting tissue for the trachea. Local neck skin was used for covering. This procedure proved very successful. 相似文献
65.
Augmentation of the nostril splint for retaining the corrected contour of the cleft lip nose 总被引:2,自引:0,他引:2
Whatever method is used to correct the deformity of the cleft lip nose, it is important to maintain the corrected contour of the nose for a certain period postoperatively. For this purpose, moldable silicone rubber is used to add volume to a ready-made nostril splint. With this material it is easy to make a splint that fits the individual contour of the nostril. Clinical examples are presented. 相似文献
66.
T Mio T Yabe M Sudoh Y Satoh T Nakajima M Arisawa H Yamada-Okabe 《Journal of bacteriology》1996,178(8):2416-2419
The CHS2 and CHS3 genes of Candida albicans were disrupted. The double disruptant was still viable. Assessment of chitin and of calcofluor white resistance shows that CHS1 is responsible for septum formation and CHS3 is responsible for overall chitin synthesis otherwise. There were only small differences in virulence to immunocompromised mice of homozygous chs2 delta amd chs3 delta null mutants. 相似文献
67.
A. SUEMORI, K. NAKAJIMA, R. KURANE AND Y. NAKAMURA. 1996. Rhodococcus erythropolis strain S1 formed enzymes essential to the degradation of phthalate when grown in phthalate-minimal medium. The reaction responsible for the dihydroxylation of the phthalate-benzene ring was concluded to be catalysed by membrane-associated phthalate 3,4-dioxygenase (PO). Of the other enzymes involved, 3,4-dihydro-3,4-dihydroxyphthalate 3,4-dehydrogenase (PH) and 3,4-dihydroxyphthalate 2-decarboxylase (PC) appeared likely to be membrane-bound, while protocatechuate 3,4-dioxygenase appeared to be present in the cytoplasm. Based on the data, the membrane-bound PO and PH apparently form an enzyme complex, which is associated with the NADH-regenerating system. 相似文献
68.
M. Sunairi N. Iwabuchi K. Murakami F. Watanabe Y. Ogawa H. Murooka M. Nakajima 《Letters in applied microbiology》1996,22(1):66-69
M. SUNAIRI, N. IWABUCHI, K. MURAKAMI, F. WATANABE, Y. OGAWA, H. MUROOKA AND M. NAKAJIMA. 1996. Suitable conditions for the introduction of bacteriophage DNA into cells of Rhodococcus rhodochrous CF222 by electroporation were established, and penicillin G was found to enhance the transfection frequency. When conditions optimal for the parental strain were applied to its colony-morphological mutants, different transfection frequencies were observed. Penicillin G enhanced the transfection frequency of smooth and mucoidal mutants but not of rough mutants. 相似文献
69.
70.
Bae Gong Young; Nakajima Nobuyoshi; Ishizuka Kozo; Kondo Noriaki 《Plant & cell physiology》1996,37(2):129-134
The rate of evolution of ethylene by tomato plants was rapidlyincreased by O3 fumigation. The time course of the increasein 1-aminocyclopropane-1-carboxylic acid (ACC) synthase activitywas the same as that in the rate of evolution of ethylene, suggestingthat ACC synthase activity might be a rate-limiting step inthe evolution of ethylene that is caused by O3 fumigation. Therate of the O3-induced evolution of ethylene was increased bythe application of ACC to tomato plants, suggesting the involvementof ACC oxidase in the O3-induced evolution of ethylene. Treatmentof plants with tiron inhibited the evolution of ethane, butnot of ethylene. These results indicated that evolution of ethylenein O3-treated tomato plants might result from enzymatic reactionscatalyzed by both ACC synthase and ACC oxidase, but not fromstimulation by O3 of the peroxidation of lipids mediated byfree radicals. Pretreatment of leaves with aminoethoxyvinylglycine (AVG), aninhibitor of ACC synthase, significantly inhibited the evolutionof ethylene that was induced by O3 and concomitantly reducedthe extent of O3-induced visible damage to leaves. Treatmentwith 2,5-norbonadiene, an inhibitor of the action of ethylene,strongly reduced the extent of visible damage caused by O3,even though it did not suppress the evloution of ethylene. Theseresults indicate that ethylene acts on certain metabolic processesto cause visible damage. (Received September 7, 1995; Accepted December 18, 1995) 相似文献