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31.
Hiroko Kawasaki Yasuo Hoshino Aiko Hirata Kazuhide Yamasato 《Archives of microbiology》1993,160(5):358-362
The 16S rRNA or rRNA gene sequences of the type strains of 5 species of Rhodobacter, Rhodopseudomonas blastica and Paracoccus denitrificans were determined. The sequence analysis revealed that Rhodobacter species, whose intracytoplasmic membrane systems were characteristically vesicular, composed a sole cluster. Rhodopseudomonas blastica, whose intracytoplasmic membrane system was lamellar, was included in the cluster of Rhodobacter. The phylogenetic co-clustering of these bacteria conformed to their possessing of the identical types of carotenoids. Paracoccus denitrificans, which is nonphototrophic, is a right member of the Rhodobacter cluster. Rhodobacter species, Rhodopseudomonas blastica and Paracoccus denitrificans are apart from the other phototrophic bacteria and have the common deletions of 21 bases at the positions 1258 to 1278 (Escherichia coli numbering system). It was demonstrated that the morphological character intracyto-plasmic membrane structure, that has been regarded as a generic criterion does not reflect the phylogeny in the phototrophic bacteria. The transfer of Rhodopseudomonas blastica to the genus Rhodobacter is proposed. 相似文献
32.
Photosynthetic Adaptation to Salt Stress in Three-Color Leaves of a C4 Plant Amaranthus tricolor 总被引:5,自引:0,他引:5
Wang Yumei; Meng Yu-Ling; Ishikawa Hiroshi; Hibino Takashi; Tanaka Yoshito; Nii Naosuke; Takabe Teruhiro 《Plant & cell physiology》1999,40(7):668-674
We examined the photosynthetic adaptation mechanisms for saltstress in Amaranthus tricolor, which has leaves with green,yellow and red regions, in relation to the accumulation of glycinebetaineas osmoprotectants. The content of Chl, especially of Chl bin the red and yellow regions was 3{small tilde}4% of that inthe green region. The levels of Chl proteins such as LHCII,PSI and PSII were significantly lower than those in the greenregion. However, the contents of other photosynthetic proteinsin these regions seem to be relatively high. We observed thenet photosynthetic CO2 fixation activity in the red and yellowregions which was about 40% of that in the green region. Uponsalt stress (0.3 M NaCl) for 5 d the levels of Chl, PSI, PSII,ribulose 1,5-bis phosphate carboxygenase and oxygenase, andthe CO2 fixation rate in the green region decreased by about20{small tilde}35% whereas those in the non-green regions remainedalmost at the same levels. A. tricolor was found to accumulatesglycinebetaine, betainealdehyde dehydrogenase and choline monooxygenaseat similar levels in all three color regions and their contentsincreased upon salt stress. These results suggest that the lowcapacity of light harvesting in non-green regions would be favorof salt stress since the photosynthetic components in theseregions were retained at relatively high levels under high salinity. (Received February 9, 1999; Accepted April 16, 1999) 相似文献
33.
Wutipraditkul N Waditee R Incharoensakdi A Hibino T Tanaka Y Nakamura T Shikata M Takabe T Takabe T 《Applied and environmental microbiology》2005,71(8):4176-4184
Aphanothece halophytica is a halotolerant alkaliphilic cyanobacterium which can grow at NaCl concentrations up to 3.0 M and at pH values up to 11. The genome sequence revealed that the cyanobacterium Synechocystis sp. strain PCC 6803 contains five putative Na+/H+ antiporters, two of which are homologous to NhaP of Pseudomonas aeruginosa and three of which are homologous to NapA of Enterococcus hirae. The physiological and functional properties of NapA-type antiporters are largely unknown. One of NapA-type antiporters in Synechocystis sp. strain PCC 6803 has been proposed to be essential for the survival of this organism. In this study, we examined the isolation and characterization of the homologous gene in Aphanothece halophytica. Two genes encoding polypeptides of the same size, designated Ap-napA1-1 and Ap-napA1-2, were isolated. Ap-NapA1-1 exhibited a higher level of homology to the Synechocystis ortholog (Syn-NapA1) than Ap-NapA1-2 exhibited. Ap-NapA1-1, Ap-NapA1-2, and Syn-NapA1 complemented the salt-sensitive phenotypes of an Escherichia coli mutant and exhibited strongly pH-dependent Na+/H+ and Li+/H+ exchange activities (the highest activities were at alkaline pH), although the activities of Ap-NapA1-2 were significantly lower than the activities of the other polypeptides. Only one these polypeptides, Ap-NapA1-2, complemented a K+ uptake-deficient E. coli mutant and exhibited K+ uptake activity. Mutagenesis experiments suggested the importance of Glu129, Asp225, and Asp226 in the putative transmembrane segment and Glu142 in the loop region for the activity. Overexpression of Ap-NapA1-1 in the freshwater cyanobacterium Synechococcus sp. strain PCC 7942 enhanced the salt tolerance of cells, especially at alkaline pH. These findings indicate that A. halophytica has two NapA1-type antiporters which exhibit different ion specificities and play an important role in salt tolerance at alkaline pH. 相似文献
34.
Laura?L?Eggink Russell?LoBrutto Daniel?C?Brune Judy?Brusslan Akihiro?Yamasato Ayumi?Tanaka J?Kenneth?HooberEmail author 《BMC plant biology》2004,4(1):5
Background
Assembly of stable light-harvesting complexes (LHCs) in the chloroplast of green algae and plants requires synthesis of chlorophyll (Chl) b, a reaction that involves oxygenation of the 7-methyl group of Chl a to a formyl group. This reaction uses molecular oxygen and is catalyzed by chlorophyllide a oxygenase (CAO). The amino acid sequence of CAO predicts mononuclear iron and Rieske iron-sulfur centers in the protein. The mechanism of synthesis of Chl b and localization of this reaction in the chloroplast are essential steps toward understanding LHC assembly. 相似文献35.
Waditee R Hibino T Tanaka Y Nakamura T Incharoensakdi A Hayakawa S Suzuki S Futsuhara Y Kawamitsu Y Takabe T Takabe T 《The Journal of biological chemistry》2002,277(21):18373-18382
Betaine is an important osmoprotectant in many plants, but its transport activity has only been demonstrated using a proline transporter from tomato, a betaine-nonaccumulating plant. In this study, two full-length and one partial transporter genes were isolated from betaine-accumulating mangrove Avicennia marina. Their homologies to betaine transporters from bacteria and betaine/4-aminobutyrate transporters from mammalian cells were low but were high to proline transporters from Arabidopsis and tomato. Two full-length transporters could complement the Na(+)-sensitive phenotype of the Escherichia coli mutant deficient in betT, putPA, proP, and proU. Both transporters could efficiently take up betaine and proline with similar affinities (K(m), 0.32-0.43 mm) and maximum velocities (1.9-3.6 nmol/min/mg of protein). The uptakes of betaine and proline were significantly inhibited by mono- and dimethylglycine but only partially inhibited by betaine aldehyde, choline, and 4-aminobutyrate. Sodium and potassium chloride markedly enhanced betaine uptake rates with optimum concentrations at 0.5 m, whereas sucrose showed only modest activation. The change of amino acids Thr(290)-Thr-Ser(292) in a putative periplasmic loop to Arg(290)-Gly-Arg(292) yielded the active transporter independent of salts, suggesting the positive charge induced a conformational change to the active form. These data clearly indicate that the betaine-accumulating mangrove contains betaine/proline transporters whose properties are distinct from betaine transporters of bacteria and mammalian cells. 相似文献
36.
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38.
It has been shown that marine algae produce the compatible solute dimethylsulfoniopropionate (DMSP) from methionine (Met) via four enzymatic reactions in which the third step, synthesis of 4‐dimethylsulfonio‐2‐hydroxy‐butyrate (DMSHB) from 4‐methylthio‐2‐hydroxybutyrate (MTHB), is the committing step. However, regulation of the biosynthetic pathways and transport properties of DMSP is largely unknown. Here, the effects of sulfur and sodium concentrations on the uptake and synthesis of DMSHB and DMSP were examined in a sterile mutant of Ulva pertusa Kjellm. Sulfur deficiency increased the activity of the sulfur assimilation enzyme O‐acetyl serine sulfhydrylase but decreased the MTHB S‐methyltransferase activity, suggesting the preferential utilization of sulfur atoms for Met metabolites other than DMSP. Uptake of DMSP and DMSHB was enhanced by S deficiency. High salinity enhanced the MTHB S‐methyltransferase activity as well as the uptake of DMSHB. The MTHB S‐methyltransferase activity was inhibited by its product DMSP. These data demonstrate the importance of MTHB S‐methyltransferase activity and uptake of DMSHB for the regulation of DMSP. 相似文献
39.
Regulation of betaine synthesis by precursor supply and choline monooxygenase expression in Amaranthus tricolor 总被引:1,自引:0,他引:1
Bhuiyan NH Hamada A Yamada N Rai V Hibino T Takabe T 《Journal of experimental botany》2007,58(15-16):4203-4212
In plants, betaine is synthesized upon abiotic stress via choline oxidation, in which choline monooxygenase (CMO) is a key enzyme. Although it had been thought that betaine synthesis is well regulated to protect abiotic stress, it is shown here that an exogenous supply of precursors such as choline, serine, and glycine in the betaine-accumulating plant Amaranthus tricolor further enhances the accumulation of betaine under salt stress, but not under normal conditions. Addition of isonicotinic acid hydrazide, an inhibitor of glycine decarboxylase, inhibited the salinity-induced accumulation of betaine. Salt-induced accumulation of A. tricolor CMO (AmCMO) and betaine was much slower in roots than in leaves, and a transient accumulation of proline was observed in the roots. Antisense expression of AmCMO mRNA suppressed the salt-induced accumulation of AmCMO and betaine, but increased the level of choline approximately 2- 3-fold. This indicates that betaine synthesis is highly regulated by AmCMO expression. The genomic DNA, including the upstream region (1.6 kbp), of AmCMO was isolated. Deletion analysis of the AmCMO promoter region revealed that the 410 bp fragment upstream of the translation start codon contains the sequence responsive to salt stress. These data reveal that the promoter sequence of CMO, in addition to precursor supply, is important for the accumulation of betaine in the betaine-accumulating plant A. tricolor. 相似文献
40.
Nakada T Saito Y Chikenji M Koda S Higuchi M Kawata K Ishida S Takahashi S Kondo S Kubota Y Kubota I Shimizu Y 《Plastic and reconstructive surgery》2006,117(2):646-51; discussion 652-3