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221.
Yoshida T Suga M Arima K Muranaka Y Tanaka T Eguchi S Lin C Yoshida S Ishikawa M Higuchi Y Seo T Ueoka Y Tomotake M Kaneda Y Darby D Maruff P Iyo M Kasai K Higuchi T Sumiyoshi T Ohmori T Takahashi K Hashimoto K 《PloS one》2011,6(5):e20469
Background
The CogState Schizophrenia Battery (CSB), a computerized cognitive battery, covers all the same cognitive domains as the Measurement and Treatment Research to Improve Cognition in Schizophrenia (MATRICS) Consensus Cognitive Battery but is briefer to conduct. The aim of the present study was to evaluate the criterion and construct validity of the Japanese language version of the CSB (CSB-J) in Japanese patients with schizophrenia.Methodology/Principal Findings
Forty Japanese patients with schizophrenia and 40 Japanese healthy controls with matching age, gender, and premorbid intelligence quotient were enrolled. The CSB-J and the Brief Assessment of Cognition in Schizophrenia, Japanese-language version (BACS-J) were performed once. The structure of the CSB-J was also evaluated by a factor analysis. Similar to the BACS-J, the CSB-J was sensitive to cognitive impairment in Japanese patients with schizophrenia. Furthermore, there was a significant positive correlation between the CSB-J composite score and the BACS-J composite score. A factor analysis showed a three-factor model consisting of memory, speed, and social cognition factors.Conclusions/Significance
This study suggests that the CSB-J is a useful and rapid automatically administered computerized battery for assessing broad cognitive domains in Japanese patients with schizophrenia. 相似文献222.
223.
224.
Yukari Terashita Kazuo Yamagata Mikiko Tokoro Fumiaki Itoi Sayaka Wakayama Chong Li Eimei Sato Kentaro Tanemura Teruhiko Wakayama 《PloS one》2013,8(10)
Somatic cell nuclear transfer to an enucleated oocyte is used for reprogramming somatic cells with the aim of achieving totipotency, but most cloned embryos die in the uterus after transfer. While modifying epigenetic states of cloned embryos can improve their development, the production rate of cloned embryos can also be enhanced by changing other factors. It has already been shown that abnormal chromosome segregation (ACS) is a major cause of the developmental failure of cloned embryos and that Latrunculin A (LatA), an actin polymerization inhibitor, improves F-actin formation and birth rate of cloned embryos. Since F-actin is important for chromosome congression in embryos, here we examined the relation between ACS and F-actin in cloned embryos. Using LatA treatment, the occurrence of ACS decreased significantly whereas cloned embryo-specific epigenetic abnormalities such as dimethylation of histone H3 at lysine 9 (H3K9me2) could not be corrected. In contrast, when H3K9me2 was normalized using the G9a histone methyltransferase inhibitor BIX-01294, the Magea2 gene—essential for normal development but never before expressed in cloned embryos—was expressed. However, this did not increase the cloning success rate. Thus, non-epigenetic factors also play an important role in determining the efficiency of mouse cloning. 相似文献
225.
Teruhiko Beppu Takeshi Uchida Yukimasa Nozaki Kei Arima 《Bioscience, biotechnology, and biochemistry》2013,77(5):1129-1136
We have made screening for antiphage antibiotics using an assay system of a male-specific filamentous phage, fl, and Escherichia coli as its host, and found a proteinous active substance named AFS (anti-filamentous phage substance) which was produced by an Actinomyces belonging to the group of Streptomyces lavendulae. It was purified from the filtered broth of the strain by ammonium sulfate precipitation and chromatographies of SP-Sephadex, CM-Sephadex and Sephadex G–50. The purified preparation was judged to be homogeneous by several column chromatographies and electrophoresis. Its molcular weight was about 5100 and isoelectric point was pH 9.9. Fourty two residues of amino acids, rich in the hydrophobic ones, were contained in one mole of AFS, but no carbohydrate was detected. 相似文献
226.
Toshiyuki Suzuki Takeshi Uozumi Teruhiko Beppu 《Bioscience, biotechnology, and biochemistry》2013,77(10):2939-2947
NADH-dependent soluble l-α-hydroxyglutarate dehydrogenase (l-2-hydroxyglutarate: NAD+ 2-oxidoreductase) was found in a bacterium belonging to the genus Alcaligenes obtained from soil by citrate enrichment culture. A mutant with about 2.5-fold higher activity of the enzyme was derived from the bacterium and used as the enzyme source. High level of the enzyme was produced at the late stage of cultivation in the presence of citrate and with limited aeration. The enzyme was purified from the cells to homogeneity to give crystals, and its enzymatic properties were studied. The enzyme strongly reduced α-ketoglutarate to stereochemically pure l-α-hydroxyglutarate with NADH as a coenzyme, but it oxidized d-α-hydroxyglutarate with about 1/10 of the rate for l-form oxidation. 相似文献
227.
Hiroo Uchiyama Takeshi Uozumi Teruhiko Beppu Kei Arima 《Bioscience, biotechnology, and biochemistry》2013,77(6):1373-1381
Prorennin-specific messenger ribonucleic acid (mRNA) has been purified by a combination of sizing techniques, including Sepharose 2B chromatography and sucrose density gradient centrifugation, and affinity chromatography with poly (U)-Sepharose, from total nucleic acid extracted from dry ice-pulverized, fourth stomach of a calf. This mRNA bound to poly (U)-Sepharose, indicating that it contained a poly (A) sequence. The total translation product in the mRNA-dependent wheat germ system, upon addition of this mRNA, was identified as authentic prorennin by gel electrophoresis. The molecular weight of this mRNA was about 3.5 × 105 as determined by gel electrophoresis. These results indicate that the synthesis of prorennin is directed by this mRNA 1,020 nucleotides in length and requires the full coding capacity of the molecule. 相似文献
228.
Shouji Ohmori Hiroshi Masai Kei Arima Teruhiko Beppu 《Bioscience, biotechnology, and biochemistry》2013,77(12):2901-2906
Industrial vinegar production by submerged acetic acid fermentation has been carried out using Acetobacter strains at about 30°C. To obtain strains suitable for acetic acid fermentation at higher temperature, about 1,100 strains of acetic acid bacteria were isolated from vinegar mash, soils in vinegar factories and fruits, and their activities to oxidize ethanol at high temperature were examined. One of these strains, No. 1023, identified as Acetobacter aceti, retained full activity to produce acetic acid in continuous submerged culture at 35°C and produced 45% of activity at 38°C, while the usual strain of A. aceti completely lost its activity at 35°C. Thus the use of this strain may reduce the cooling costs of industrial vinegar production. 相似文献
229.
Induction of some plant organs including tubers and flower buds begins with sensing environmental cues, such as photoperiod
and temperature in the leaves. Theobroxide has been shown to induce potato tuberization and flower-bud formation in morning
glory under non-inductive photoperiodic conditions, stimulating the activity of lipoxygenase (LOX) and the synthesis of jasmonic
acid (JA). In the present study, the ability of theobroxide to overcome the inhibitory effect of unfavorable high temperature
on the induction of tubers in potato and flower buds in morning glory was examined. Both tuber induction and flower-bud formation
under non-inductive high temperatures were promoted by the application of theobroxide at a high concentration. However, although
theobroxide treatment resulted in an increase in fresh weight during potato tuber growth at 30°C, morning glory plants treated
with theobroxide at 35°C failed to bloom, implying that theobroxide may assist only in flower-bud formation. 相似文献
230.
Tracking epigenetic histone modifications in single cells using Fab-based live endogenous modification labeling 总被引:1,自引:0,他引:1
Hayashi-Takanaka Y Yamagata K Wakayama T Stasevich TJ Kainuma T Tsurimoto T Tachibana M Shinkai Y Kurumizaka H Nozaki N Kimura H 《Nucleic acids research》2011,39(15):6475-6488
Histone modifications play an important role in epigenetic gene regulation and genome integrity. It remains largely unknown, however, how these modifications dynamically change in individual cells. By using fluorescently labeled specific antigen binding fragments (Fabs), we have developed a general method to monitor the distribution and global level of endogenous histone H3 lysine modifications in living cells without disturbing cell growth and embryo development. Fabs produce distinct nuclear patterns that are characteristic of their target modifications. H3K27 trimethylation-specific Fabs, for example, are concentrated on inactive X chromosomes. As Fabs bind their targets transiently, the ratio of bound and free molecules depends on the target concentration, allowing us to measure changes in global modification levels. High-affinity Fabs are suitable for mouse embryo imaging, so we have used them to monitor H3K9 and H3K27 acetylation levels in mouse preimplantation embryos produced by in vitro fertilization and somatic cell nuclear transfer. The data suggest that a high level of H3K27 acetylation is important for normal embryo development. As Fab-based live endogenous modification labeling (FabLEM) is broadly useful for visualizing any modification, it should be a powerful tool for studying cell signaling and diagnosis in the future. 相似文献