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71.
We have developed a biochemical approach for identifying the components of cortical actin assembly sites in polarized yeast cells, based on a permeabilized cell assay that we established for actin assembly in vitro. Previous analysis indicated that an activity associated with the cell cortex promotes actin polymerization in the bud. After inactivation by a chemical treatment, this activity can be reconstituted back to the permeabilized cells from a cytoplasmic extract. Fractionation of the extract revealed that the reconstitution depends on two sequentially acting protein factors. Bee1, a cortical actin cytoskeletal protein with sequence homology to Wiskott-Aldrich syndrome protein, is required for the first step of the reconstitution. This finding, together with the severe defects in actin organization associated with the bee1 null mutation, indicates that Bee1 protein plays a direct role in controlling actin polymerization at the cell cortex. The factor that acts in the second step of the reconstitution has been identified by conventional chromatography. It is composed of a novel protein, Pca1. Sequence analysis suggests that Pca1 has the potential to interact with SH3 domain-containing proteins and phospholipids. 相似文献
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The mutation, oligosyndactyly, results in syndactyly, muscle anomalies, and diabetes insipidus in heterozygous mice. When homozygous, the mutation is lethal early in development. Although homozygous embryos are able to form blastocyst outgrowths (the in vitro equivalent to implantation), cells begin to accumulate in mitosis as early as the blastocyst stage. Even though the cytologic appearance is that of mitotic cells treated with a microtubule inhibitor such as colcemid, the homozygous embryos do, in fact, have normal appearing mitotic spindles. These results define the Os mutation as one which, in the homozygous state, prevents the movement of chromosomes from the metaphase plate. It is the first mammalian developmental mutation to be so defined and is unique among all mitotic arrest mutations thus far described in higher eucaryotes. 相似文献
74.
Methanogenic Activity and Structural Characteristics of the Microbial Biofilm On a Needle-Punched Polyester Support 总被引:2,自引:0,他引:2
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Martin Harvey Cecil W. Forsberg Terry J. Beveridge Jack Pos John R. Ogilvie 《Applied microbiology》1984,48(3):633-638
In a downflow stationary fixed-film anaerobic reactor receiving a swine waste influent, few bacteria were observed to be tightly adherent to the surfaces of the needle-punched polyester support material. However, there was a morphologically complex, dense population of bacteria trapped within the matrix. Frequently large microcolonies of a uniform morphological type of bacteria were observed. These were particularly evident for methanosarcina-like bacteria which grew forming large aggregates of unseparated cells. Leafy deposits of electron-dense, calcium- and phosphorus-enriched material coated the polyester matrix and some cells. As the biofilm matured there was more extensive mineral deposition which completely entrapped cells. The entrapped cells appeared to autolyze, and many were partially degraded. Further impregnation of the matrix with minerals and apparent cell death may eventually have a deleterious effect on the methanogenic activity of the biofilm. 相似文献
75.
Terry W. Spithill Raelene J. Grumont Graham F. Mitchell 《Journal of cellular biochemistry》1984,24(2):103-112
The genetic characterization of pathogenic isolates of Leishmania was attempted by analysis of the molecular properties of kinetoplast DNA (kDNA) minicircles. Unit minicircle size is not conserved during speciation of Leishmania since the minicircles of strains and clones of L t major are smaller (700 bp) than those found in certain strains of L mexicana ssp (820 bp), L donovani (850 bp) or L t tropica (900 bp). Schizodeme analysis of minicircles reveals a high degree of sequence divergence in kDNA of Leishmania with the degree of microheterogeneity varying between species. This sequence divergence allows the discrimination of species, strains, and clones of Leishmania into schizodemes. Southern blot hybridization experiments reveal that at high stringency overall minicircle sequence homology is conserved among clones and strains of one species (L t major) but not between different species. This property of minicircle DNA permits the use of kDNA probes as a species-specific diagnostic test for the identification of unknown Leishmania isolates. The properties of kDNA from an L t tropica strain LRC-L32 (a “recidiva” organism) are so diverged from those of L t major strains as to support the classification [22,23] of L t tropica and L t major as separate species of Leishmania rather than subspecies of L tropica. 相似文献
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Terry W. Lucansky 《American journal of botany》1977,64(3):253-259
Comparative anatomical studies of the mature stems of two species each of Trichipteris and Cyathea (Cyatheaceae) are described. The outermost boundary of the stem is typically a two-layered hypodermis. Mucilage-sac cells are randomly distributed in all parenchymatous areas of the stem and form articulated laticifer systems. Localized areas of sclerenchyma tissue occur in the cortex of both T. microphylla and C. suprastrigosa. All species studied possess medullary bundles, whereas cortical bundles are found only in T. trichiata. Accessory bundles occasionally are associated with indentations in the internal stelar sheath of T. trichiata. The stelar pattern in each genus is a dictyostele and consists of individual meristeles. Distinctive cubical cells typically occur wherever sclerenchymatous fibers and parenchyma cells abut one another. Tangential cells occur within the primary phloem of each meristele, and occasionally within the larger accessory bundles. The primary xylem of the adventitious roots is typically diarch, although triarch and tetrarch xylem may occur. Leaf traces and petiole strands are similar anatomically to the accessory bundles. Based upon this study Trichipteris and Cyathea show striking anatomical similarities, and appear to be closely-related taxa. 相似文献
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Terry M. Caelli 《Biological cybernetics》1981,39(2):97-103
In a series of experiments we have investigated the perception of Moiré patterns as a function of spatial density, rotation and temporal display parameters. Results indicate that the local correlation extraction process involved in the perception of these patterns is not feature specific, yet is driven by excitatory (correlated) and inhibitory (uncorrelated) information under a form of spatial summation. These results are comparable with recent results on texture discrimination where texture interpoint distance distributions (dipole statistics) have also been discovered to have excitatory and inhibitory components. 相似文献
80.
Terry W. Moody Duncan P. Taylor Candace B. Pert 《Journal of cellular biochemistry》1981,15(2):153-159
The effect of nucleotides on central nervous system neuropeptide receptor binding was investigated. The guanine nucleotides, guanosine-5′-triphosphate and guanylyl-5′-imidodiphosphate, significantly inhibited the binding of radiolabeled vasoactive intestinal polypeptide but not that of [Tyr4]bombesin to rat brain membranes. Vasoactive intestinal polypeptide binding was inhibited by guanine nucleotides in a dose-dependent manner. Using a 20 μM dose, 60% of the specific vasoactive intestinal polypeptide binding was inhibited by guanylyl-5′-imidodiphosphate, which was more potent than guanosine-5′-triphosphate, whereas other nucleotides were not effective. This reduction in binding was a consequence of lower affinity of the receptor for vasoactive intestinal polypeptide, which in turn resulted from an increased rate of dissociation. 相似文献