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排序方式: 共有140条查询结果,搜索用时 343 毫秒
91.
da Silva Lawisch Gabriela Kniphoff Biolchi Vanderlei Kaufmann Gabriela Nicolai Gabriela Capitaneo Eduarda Rosembach Thais Regina Zang Janaina Brum Ilma Simoni Chies José Artur Bogo 《Molecular biology reports》2022,49(10):9445-9451
Molecular Biology Reports - Prostate cancer (PCA) is one of the leading causes of death among men, being related to several factors, including the aging male population, like benign prostatic... 相似文献
92.
The application of electric pulses in cells increases membrane permeability. This phenomenon is called electroporation. Current electroporation models do not explain all experimental findings: part of this problem is due to the limitations of numerical methods. The Equivalent Circuit Method (ECM) was developed in an attempt to solve electromagnetic problems in inhomogeneous and anisotropic media. ECM is based on modeling of the electrical transport properties of the medium by lumped circuit elements as capacitance, conductance, and current sources, representing the displacement, drift, and diffusion current, respectively. The purpose of the present study was to implement a 2-D cell Model Development Environment (MDE) of ionic transport process, local anisotropy around cell membranes, biological interfaces, and the dispersive behaviour of tissues. We present simulations of a single cell, skeletal muscle, and polygonal cell arrangement. Simulation of polygonal form indicates that the potential distribution depends on the geometrical form of cell. The results demonstrate the importance of the potential distributions in biological cells to provide strong evidences for the understanding of electroporation. 相似文献
93.
Brady R. Cunningham Jennifer R. Brum Sarah M. Schwenck Matthew B. Sullivan Seth G. John 《Applied and environmental microbiology》2015,81(9):2995-3000
Viruses affect biogeochemical cycling, microbial mortality, gene flow, and metabolic functions in diverse environments through infection and lysis of microorganisms. Fundamental to quantitatively investigating these roles is the determination of viral abundance in both field and laboratory samples. One current, widely used method to accomplish this with aquatic samples is the “filter mount” method, in which samples are filtered onto costly 0.02-μm-pore-size ceramic filters for enumeration of viruses by epifluorescence microscopy. Here we describe a cost-effective (ca. 500-fold-lower materials cost) alternative virus enumeration method in which fluorescently stained samples are wet mounted directly onto slides, after optional chemical flocculation of viruses in samples with viral concentrations of <5 × 107 viruses ml−1. The concentration of viruses in the sample is then determined from the ratio of viruses to a known concentration of added microsphere beads via epifluorescence microscopy. Virus concentrations obtained by using this wet-mount method, with and without chemical flocculation, were significantly correlated with, and had precision equivalent to, those obtained by the filter mount method across concentrations ranging from 2.17 × 106 to 1.37 × 108 viruses ml−1 when tested by using cultivated viral isolates and natural samples from marine and freshwater environments. In summary, the wet-mount method is significantly less expensive than the filter mount method and is appropriate for rapid, precise, and accurate enumeration of aquatic viruses over a wide range of viral concentrations (≥1 × 106 viruses ml−1) encountered in field and laboratory samples. 相似文献
94.
Gemelli T Carvalho CA de Andrade RB Guerra RB Oliboni L Salvador M Dani C Funchal C 《Molecular and cellular biochemistry》2011,355(1-2):167-172
The objective of this study was to investigate the in vitro effects of the organochalcogen 3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one on some parameters of oxidative stress in liver, kidney, and heart of 10-day-old rats. The homogenates of liver, kidney, and heart were incubated for 1 h in the absence (control) or in the presence of 1, 10, or 30 μM of the organoselenium and thiobarbituric acid reactive substances, carbonyl, and the activity of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) were measured. First, we tested the influence of the compound on 1,1-diphenyl-2-picrylhydrazyl (DPPH(?)) radical scavenging and verified that the organochalcogen did not have any antioxidant properties. We observed an increase of lipid peroxidation in all concentrations tested in heart and kidney, while in liver only in the concentrations of 10 and 30 μM. Moreover, we also verified an enhance of protein oxidation in the concentrations of 10 and 30 μM in kidney. On the other hand, the compound caused a reduction on the activity of CAT in heart (10 and 30 μM), liver (30 μM), and kidney (30 μM). The activity of SOD was increased in heart (10 and 30 μM), while in liver (30 μM) and in kidney (10 and 30 μM) the activity was reduced. Our findings indicate that this organoselenium compound induces oxidative stress in liver, heart, and kidney of immature rats, collaborating to the fact that these tissues are potential targets for the organochalcogen action. 相似文献
95.
The aim of this study was to investigate biohydrogen production from biofuel-reforming processes using new multi-component bulk-type cobalt-based catalysts. The addition of different components to improve the catalytic performance was studied. Monometallic cobalt catalyst and catalysts containing Ru (ca. 1%) and/or Na (ca. 0.5%) were characterized and tested in the 623-673 K temperature range in ethanol steam reforming (ESR) with a steam/carbon ratio (S/C) of 3. The catalysts showed a high performance for hydrogen production and, except for H(2) and CO(2), only small amounts of by-products were obtained, depending on the temperature and the catalyst used. The catalyst containing both Ru and Na (Co-Ru(Na)) showed the best catalytic behavior in ESR. It operated stably for at least 12 days under cycles of oxidative steam reforming of glycerol/ethanol mixtures (S/C=2) and activation under O(2). 相似文献
96.
We investigate the use of dispersive surface energy in quantifying surface amorphous content, and the concept of effective
amorphous surface area is introduced. An equation is introduced employing the linear combination of surface area normalized
square root dispersive surface energy terms. This equation is effective in generating calibration curves when crystalline
and amorphous references are used. Inverse gas chromatography is used to generate dispersive surface energy values. Two systems
are investigated, and in both cases surface energy data collected for physical mixture samples comprised of amorphous and
crystalline references fits the predicted response with good accuracy. Surface amorphous content of processed lactose samples
is quantified using the calibration curve, and interpreted within the context of effective amorphous surface area. Data for
bulk amorphous content is also utilized to generate a thorough picture of how disorder is distributed throughout the particle.
An approach to quantifying surface amorphous content using dispersive surface energy is presented. Quantification is achieved
by equating results to an effective amorphous surface area based on reference crystalline, and amorphous materials. 相似文献
97.
98.
dos Santos Lacerda D de Oliveira Castro V Mascarenhas M Guerra RB Dani C Coitinho A Gomez R Funchal C 《Cell biochemistry and function》2012,30(4):315-319
Organochalcogens are extensively produced and employed by industry and agriculture, and the risk of occupational and environmental toxicity to them has been poorly understood. Here, we investigated the acute effect of a new organochalcogen 3-methyl-1-phenyl-2-(phenylseleno)oct-2-en-1-one on biochemical and hematological parameters in male Wistar rats. The animals were treated with a single intraperitoneal injection of the organochalcogen at doses of 125, 250 or 500 μg·kg(-1). After 60 min, the animals were sacrificed by decapitation, and the trunk blood was collected for determination of glucose, triglycerides, cholesterol, alanine aminotransferase (ALT), aspartate aminotransferase, lactate dehydrogenase, urea, creatinine, C-reactive protein, red blood cells, hematocrit, hemoglobin and white blood cells (WBC). Our results showed a reduction in cholesterol levels in all treated groups, an increase in ALT activity at doses of 250 and 500 μg·kg(-1), a decrease of hemoglobin and an increase in WBC in animals that received 250 and 500 μg·kg(-1) of the organoselenium. In addition, we observed an increase in neutrophil counts at 125 μg·kg(-1) dose and a decrease at 500 μg·kg(-1) dose. We also verified an increase in lymphocyte counts at the dose of 500 μg·kg(-1). Thus, the present study shows that the acute treatment with this new organochalcogen causes biochemical changes and hematological disorders in male rats. 相似文献
99.
100.
Dalla Rosa L Da Silva AS Oliveira CB Brum I Benevenutti E Dorneles F Jaques JA Tavares KC Miletti LC Leal MR Monteiro SG 《Experimental parasitology》2012,131(3):358-362
The aim of this study was to evaluate the effects of a treatment using injectable zinc and copper in rats infected with Trypanosoma evansi. 48 rats were divided into eight groups of six animals each. Group A was composed of uninfected animals. Animals from groups B-H were inoculated at the 5th day of experiment with 1.2×10(6) trypanosomes. Group B was used as a positive control. The infected groups received prophylactic (C, D and E) and therapeutic (F, G and H) treatments with the zinc and copper, both at a dose of 5 mg kg(-1). The effectiveness of treatment was confirmed by negative blood smears and Polymerase Chain Reaction (PCR) at the end of study. All treated animals had their prepatent period and survival prolonged when compared with control group (group B). Treatment efficacy was 17% (C: zinc), 33% (D: copper), 50% (E: zinc+copper), 0% (F: zinc), 50% (G: copper) and 50% (H: zinc+copper). Thus, we can conclude that treatment with zinc and copper are capable of controlling and/or curing T. evansi infection in rats, delaying the parasitemia and prolonging their survival. 相似文献