全文获取类型
收费全文 | 389篇 |
免费 | 28篇 |
专业分类
417篇 |
出版年
2022年 | 4篇 |
2021年 | 4篇 |
2020年 | 1篇 |
2019年 | 4篇 |
2018年 | 4篇 |
2017年 | 9篇 |
2016年 | 10篇 |
2015年 | 18篇 |
2014年 | 22篇 |
2013年 | 26篇 |
2012年 | 32篇 |
2011年 | 17篇 |
2010年 | 15篇 |
2009年 | 26篇 |
2008年 | 18篇 |
2007年 | 19篇 |
2006年 | 21篇 |
2005年 | 25篇 |
2004年 | 20篇 |
2003年 | 18篇 |
2002年 | 11篇 |
2001年 | 18篇 |
2000年 | 14篇 |
1999年 | 8篇 |
1998年 | 13篇 |
1997年 | 7篇 |
1996年 | 4篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1992年 | 1篇 |
1991年 | 4篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 2篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1976年 | 2篇 |
1971年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有417条查询结果,搜索用时 0 毫秒
81.
Martinus AM van Boekel Erik R Vossenaar Frank HJ van den Hoogen Walther J van Venrooij 《Arthritis research & therapy》2001,3(2):1-7
This review focuses on the mechanisms of stress response in the synovial tissue of rheumatoid arthritis. The major stress factors, such as heat stress, shear stress, proinflammatory cytokines and oxidative stress, are discussed and reviewed, focusing on their potential to induce a stress response in the synovial tissue. Several pathways of stress signalling molecules are found to be activated in the synovial membrane of rheumatoid arthritis; of these the most important examples are heat shock proteins, mitogen-activated protein kinases, stress-activated protein kinases and molecules involved in the oxidative stress pathways. The expression of these pathways in vitro and in vivo as well as the consequences of stress signalling in the rheumatoid synovium are discussed. Stress signalling is part of a cellular response to potentially harmful stimuli and thus is essentially involved in the process of synovitis. Stress signalling pathways are therefore new and promising targets of future anti-rheumatic therapies. 相似文献
82.
Aimee M. Terauchi Graham Peers Marilyn C. Kobayashi Krishna K. Niyogi Sabeeha S. Merchant 《Photosynthesis research》2010,105(1):39-49
To investigate the impact of iron deficiency on bioenergetic pathways in Chlamydomonas, we compared growth rates, iron content,
and photosynthetic parameters systematically in acetate versus CO2-grown cells. Acetate-grown cells have, predictably (2-fold) greater abundance of respiration components but also, counter-intuitively,
more chlorophyll on a per cell basis. We found that phototrophic cells are less impacted by iron deficiency and this correlates
with their higher iron content on a per cell basis, suggesting a greater capacity/ability for iron assimilation in this metabolic
state. Phototrophic cells maintain both photosynthetic and respiratory function and their associated Fe-containing proteins
in conditions where heterotrophic cells lose photosynthetic capacity and have reduced oxygen evolution activity. Maintenance
of NPQ capacity might contribute to protection of the photosynthetic apparatus in iron-limited phototrophic cells. Acetate-grown
iron-limited cells maintain high growth rates by suppressing photosynthesis but increasing instead respiration. These cells
are also able to maintain a reduced plastoquinone pool. 相似文献
83.
84.
Takenori Yamamoto Satsuki Terauchi Aiko Tachikawa Kikuji Yamashita Masatoshi Kataoka Hiroshi Terada Yasuo Shinohara 《Journal of bioenergetics and biomembranes》2005,37(5):299-307
N,N′-dicyclohexylcarbodiimide (DCCD) was earlier reported to have stimulatory effects on mitochondrial respiration and to induce
mitochondrial swelling, when it was added to mitochondrial suspensions. These data seem to imply that DCCD caused the mitochondrial
permeability transition (PT), but this possibility had never been investigated. In the present study, effects of DCCD on the
mitochondrial structure and function were studied in detail. DCCD was found to induce mitochondrial PT in a cyclosporine A-insensitive
manner. Electron microscopic analysis also supported the induction of the mitochondrial PT by DCCD. However, different from
many other PT inducers, DCCD failed to cause massive release of mitochondrial cytochrome c. To understand the relationship between the induction of mitochondrial PT and the release of mitochondrial cytochrome c, we compared the actions of DCCD on mitochondrial structure and function with those of Ca2+, known as an ordinary PT inducer. As a result, two parameters considered to be critical for controlling the release of mitochondrial
cytochrome c on the induction of PT were mitochondrial volume and the velocity of mitochondrial oxygen consumption. 相似文献
85.
Sequence variation at the alcohol dehydrogenase (Adh) locus was analyzed for six species each of the genera Arabis and Arabidopsis. Phylogenetic analysis showed that investigated species were grouped into three clusters, and the generic classification did not correspond to the clusterings. The results indicated that the genera could not be distinguished on the basis of the Adh variation. A significant difference in the ratio of silent to replacement sites was detected by MK test in two comparisons, with Arabidopsis thaliana polymorphism due to excess silent divergence. Silent changes were predominant in the evolution of the Adh locus in Arabis and Arabidopsis. To infer evolutionary significance of silent substitutions, codon bias was studied. The degree of codon bias of the Adh region was relatively constant over Arabis and Arabidopsis species. "Preferred" codons of A. thaliana were determined. No evidence of natural selection on codon change was detected in the Adh regions of A. thaliana and Arabis gemmifera. 相似文献
86.
Roxana Portieles Eduardo Canales Ingrid Hernández Yunior López Mayra Rodríguez Meilyn Rodríguez Ryohei Terauchi Carlos Borroto Ramón Santos Camilo Ayra-Pardo Deqiang Zhu Xinli Liu Orlando Borrás-Hidalgo 《Plant Molecular Biology Reporter》2018,36(3):484-490
The oomycete pathogens produce important diseases in many plant species. To identify extensin genes expressed during the oomycete Phytophthora nicotianae-Nicotiana megalosiphon interaction, we used the SuperSAGE technology. Using this approach, we detected a N. megalosiphon extensin gene (NmEXT) triggered during the interaction. The extensin gene accumulation induced by the pathogen correlated with disease resistance in different Nicotiana species. Transient expression of NmEXT gene in susceptible Nicotiana tabacum enhanced the resistance to P. nicotianae. Our date indicated that NmEXT gene served a positive role in N. tabacum resistance against P. nicotianae. 相似文献
87.
Takeshi Wakoh Kunihiko Terauchi Akihito Ishigami Mitsuo Maruyama 《Biochemical and biophysical research communications》2009,380(4):807-812
A novel target of NESH-SH3 (TARSH) was identified as a cellular senescence related gene in mouse embryonic fibroblasts (MEFs) replicative senescence, the expression of which has been suppressed in primary clinical lung cancer specimens. However, the molecular mechanism underlying the regulation of TARSH involved in pulmonary tumorigenesis remains unclear. Here we demonstrate that the reduction of TARSH gene expression by short hairpin RNA (shRNA) system robustly inhibited the MEFs proliferation with increase in senescence-associated β-galactosidase (SA-β-gal) activity. Using p53−/− MEFs, we further suggest that this growth arrest by loss of TARSH is evoked by p53-dependent p21Cip1 accumulation. Moreover, we also reveal that TARSH reduction induces multicentrosome in MEFs, which is linked in chromosome instability and tumor development. These results suggest that TARSH plays an important role in proliferation of replicative senescence and may serve as a trigger of tumor development. 相似文献
88.
A pivotal role for DNase I-sensitive regions 3b and/or 4 in the induction of somatic hypermutation of IgH genes. 总被引:4,自引:0,他引:4
A Terauchi K Hayashi D Kitamura Y Kozono N Motoyama T Azuma 《Journal of immunology (Baltimore, Md. : 1950)》2001,167(2):811-820
Chimeric mice were prepared from embryonic stem cells transfected with IgH genes as transgenes and RAG-2-deficient blastocysts for the purpose of identifying the cis-acting elements responsible for the induction of somatic hypermutation. Among the three transgene constructs used, the V(H) promoter, the rearranged V(H)-D-J(H), an intron enhancer/matrix attachment region, and human Cmu were common to all, but the 3'-untranslated region in each construct was different. After immunization of mice with a T cell-dependent Ag, the distribution and frequency of hypermutation in transgenes were analyzed. The transgene lacking the 3' untranslated region showed a marginal degree of hypermutation. Addition of the 3' enhancer resulted in a slight increase in the number of mutations. However, the transgene containing DNase I-sensitive regions 3b and 4 in addition to the 3' enhancer showed more than a 10-fold increase in hypermutation, reaching levels comparable to those observed in endogenous V(H)186.2 genes of C57BL/6 mice. 相似文献
89.
90.
A case report of a patient with retinoblastoma and chromosome 13q deletion: assignment of a new gene (gene for LCP1) on human chromosome 13 总被引:2,自引:0,他引:2
I. Kondo K. Shin S. Honmura H. Nakajima E. Yamamura H. Satoh M. Terauchi Y. Usuki H. Takita H. Hamaguchi 《Human genetics》1985,71(3):263-266
Summary Retinoblastoma (Rb) occurs in hereditary, non-hereditary, and chromosomal deletion forms and the locus for the Rb gene (Rb-1) is closely linked to the locus for esterase D (ESD) assigned to the chromosome 13q14.11. We describe a patient who was predicted to have Rb from the genetic analysis of the
chromosome and ESD phenotype. Furthermore, the gene for lymphocyte cytosol polypeptide with molecular weight of 64,000 (LCP1:
McKusick catalogue No. 15343, 1983) was assigned to chromosome 13 by deletion mapping. A 3-month-old female had many characteristics
of chromosome 13q-syndrome, including dolichocephaly, epicanthus, ptosis, depressed nasal bridge, micrognathia, short webbed
neck, and short fifth fingers with clinodactyly and single crease. The karyotype of the patient was 46,XX,del(13) (q14.1–q32),
though both the parents had normal karyotypes. As expected, the phenotype of ESD derived from one of the parents, the father
in this case, was not detected in peripheral blood lymphocytes by two-dimensional gel electrophoresis (two-DE), indicating
that ESD from the father was deleted in the abnormal chromosome 13. The possibility of paternity was calculated to be 0.996 based
on the data using 22 genetic markers. Bilateral retinoblastomas could be diagnosed by ophthalmologic examinations before the
manifestation of any clinical signs of the tumor and immediately intensive care was taken. In addition, the phenotype of LCP1
derived from the father was not expressed in the lymphocyte proteins from the patient. These data indicate that the gene for
LCP1 (LCP1) is located in the region q14.1–q32 of chromosome 13 and may be a useful genetic marker for preclinical diagnosis of Rb. 相似文献