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71.
T Watanabe Y Hashimoto T Teramoto S Kume C Naito H Oka 《Archives of biochemistry and biophysics》1986,246(2):699-709
We tested the effects of calmodulin, two types of calmodulin antagonists, and various phospholipids on the phospholipase A2 activities of intact platelets, platelet membranes, and partially purified enzyme preparations. Trifluoperazine, chlorpromazine (phenothiazines) and N-(6-amino-hexyl)-5-chloro-1-naphthalenesulfonamide (W-7), at concentrations which antagonize the effects of calmodulin, significantly inhibited thrombin- and Ca2+ ionophore-induced production of arachidonic acid metabolites by suspensions of rabbit platelets and Ca2+-induced arachidonic acid release from phospholipids of membrane fractions, but not phospholipase A2 activity in purified enzyme preparations. The addition of acidic phospholipids, but not calmodulin, stimulated phospholipase A2 activity in purified enzyme preparations while decreasing its Km for Ca2+. The dose-response and kinetics of inhibition by calmodulin antagonists of acidic phospholipid-activated phospholipase A2 activity in purified preparations were similar to those of Ca2+-induced arachidonic acid release from membrane fractions. Calmodulin antagonists were also found to inhibit Ca2+ binding to acidic phospholipids in a similar dose-dependent manner. Our results suggest that the platelet phospholipase A2 is the key enzyme involved in arachidonic acid mobilization in platelets and is regulated by acidic phospholipids in a Ca2+-dependent manner and that calmodulin antagonists inhibit phospholipase A2 activity via an action on acidic phospholipids. 相似文献
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Pituitary adenoma tissues are hypovascular, and have a lower partial oxygen pressure compared with neighboring normal organs. In this study, we investigated whether hypoxia influences the cell invasiveness of the human pituitary adenoma cell line, HP-75. HP-75 cells were exposed to hypoxic (1–10% oxygen) or normoxic (21% oxygen) conditions for 24 hours. Gelatin and reverse zymogram assays were used to determine the enzyme activities of matrix metalloproteinases (MMP) and tissue inhibitor of metalloproteinases (TIMP). Cell adhesion and Matrigel cell invasion were examined with a Boiden chamber. Finally, the mRNA gene expression profiles of cells exposed to hypoxia or normoxia were examined by cDNA microarray and confirmed with real-time RT-PCR and flow cytometry. The gelatin and reverse zymograms revealed that the activities of MMP and TIMP were not significantly altered by hypoxia. Matrigel cell invasion and cell adhesion to Matrigel or collagen type IV were increased by hypoxia (3.8- and 4.8-fold, respectively). The cDNA microarray analysis revealed that laminin β2 chain mRNA was specifically up-regulated under hypoxic conditions (4.96-fold). Finally, real-time RT-PCR and flow cytometry verified the elevated expression of laminin β2 chain at the mRNA and protein levels under hypoxic conditions. RNA interference with siRNA targeting laminin β2 inhibited Matrigel invasion and adhesion to collagen type IV in a dose.dependent manner.Collectively, these results suggested that hypoxia (1% oxygen) enhanced the cell invasion properties of a pituitary adenoma cell line in association with elevated expression of laminin β2 and enhanced binding to collagen type IV.Key Words: cell invasion, hypoxia, laminin β2, pituitary adenoma, siRNA 相似文献
74.
Rapid identification of bacteria by a bioinformatics-based approach, which processes the mass spectra observed by matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), relies on the calculated masses of ribosomal subunit proteins as biomarkers predicted from amino acid sequences found in protein sequence databases. To verify the actual state of the registered sequence information, a simple intact protein analysis by MALDI-MS using cell lysates as samples was applied to the characterization of ribosomal proteins from genome-sequenced Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus strains. This method avoided the risk of loss of some subunit proteins and the formation of disulfide bonds during the purification of ribosomal proteins. By comparing this with the MALDI mass spectra of different strains and carrying out manual inspection of sequence information, a total of five errors in N-terminal amino acid sequences were identified. After sequence correction, approximately 40 out of 53 subunit proteins could be assigned, considering N-terminal methionine loss only as a post-translational modification. These show promise for use as practical biomarkers for the rapid identification of S. thermophilus and L. bulgaricus. After verification of these amino acid sequences, mass differences relative to those of genome-sequenced strains have the potential for distinguishing bacteria at the strain level. 相似文献
75.
Cellulose derivatives having a cross-linkable mercapto group were prepared by esterification of cellulose acetate (CA) with mercaptoacetic acid. The molecular structure of a series of products (CA-MA) was characterized by (1)H and (1)H- (13)C HMQC NMR spectroscopy and gel permeation chromatography. The solubility of CA-MA in water and organic solvents could be controlled by changing the preparation conditions including the degree of acetyl substitution of the starting CA. The CA-MA samples thus synthesized showed a sol-gel transition in solution and a shape memory-recovery behavior in film form through adequate redox treatments due to the reversible, cross-linking association and dissociation between mercapto groups. Dimethyl sulfoxide was usable as the organic solvent and oxidant, while the major reducing reagent was 2-mercaptoethanol or ammonium mercaptoacetic acid. The progress of the redox reactions was followed by using a confocal depth scanning technique in Raman spectroscopy. It was found that the compatibility between the cellulose derivatives and the redox reagents used was an important factor for the successful reactions, especially in the samples of film form. The cross-linking effect on the thermal and viscoelastic properties of the CA-MA films was also estimated by differential scanning calorimetry and dynamic mechanical analysis. Discussion focused on the alternately declining and recovering behavior of a principal loss tan delta peak, observed following the redox treatments repeated for the CA-MA film specimens. 相似文献
76.
Nobuyuki Kutsukake Migaku Teramoto Seijiro Homma Yusuke Mori Kazunari Matsudaira Hisao Kobayashi Takafumi Ishida Kazuo Okanoya Toshikazu Hasegawa 《Ethology : formerly Zeitschrift fur Tierpsychologie》2012,118(3):269-280
It has been established that various species exhibit personality, defined as intra‐individual consistency and inter‐individual variation in behavioural phenotypes. For example, certain individuals may demonstrate consistently greater behavioural reactions and elevated stress responses. We conducted playback experiments and hormonal analyses on male chimpanzees (Pan troglodytes) in captivity to investigate the patterns and proximate mediators of individual variations in behavioural reactions. We found intra‐individual consistency and inter‐individual variation in behavioural reactions (intensive vigilance towards the direction of speakers) to vocalisations by unfamiliar chimpanzees. This behavioural reaction was positively correlated with changes in salivary cortisol concentration, suggesting that stress is a proximate factor mediating the variation in behavioural reactions. The males who were highly responsive to the conspecific vocalisation also exhibited high behavioural reactions towards the neutral broadcast stimulus (the jungle crow’s Corvus macrorhynchos ‘ka’ vocalisation). This observation is consistent with the notion that male chimpanzees vary in intrinsic behavioural tendency to different stimuli. 相似文献
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Mechanical micro/nanofibrillation of Douglas fir was performed by a continuous extrusion process in an attempt to develop a cost-effective pretreatment method for enzymatic saccharification. Additives with cellulose affinity (ethylene glycol, glycerol, and dimethyl sulfoxide) were used to effectively fibrillate the wood cell wall into submicron- or nano-scale, thus opening up the cell wall structure for improving enzymatic accessibility, and lower the extrusion torque. Morphological characterization showed that ethylene glycol was the most effective additive for fibrillation. The fibrillated products were converted into glucose with a high yield by enzymatic saccharification. The maximum cellulose-to-glucose conversion was achieved when ethylene glycol was used; the value was 62.4%. The glucose yield was approximately 6 times higher than that of the untreated raw material. 相似文献
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