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排序方式: 共有423条查询结果,搜索用时 15 毫秒
421.
M. A. Rojo F. J. Arias R. Iglesias J. M. Ferreras R. Muñoz T. Girbés 《Physiologia plantarum》1993,88(4):549-556
A cell-free translation system was prepared from 3- to 5-day-old embryonic axes of gherkin ( Cucumis sativus L.). The system was optimized for Mg2+ , K+ , NH+ 4 , high speed supernatants, tRNA mixture from wheat germ, time and temperature. The system translates efficiently both endogenous mRNA (using a 30000 g supernatant) and polyuridylic acid (using either a 30000 g supernatant or a 100000 g supernatant supplemented with purified ribosomes). Translation by gherkin ribosomes was inhibited by several well-known eukaryotic inhibitors, antibiotics and ribosome-inactivating proteins. A translational inhibitory activity found in Cucumis sativus L. dry seeds acted on polypeptide synthesis carried out by cell-free systems from several mammals and plants, including gherkin embryonic axes. Our results indicate that the inhibitor is located in the seed bark and cotyledons, and is either blocked or absent in the embryonic axes, thus allowing the isolation of active gherkin ribosomes. The presence of the putative inhibitor appeared to be unevenly distributed in developing plants. 相似文献
422.
A. Bódalo Santoyo J. Bastida Rodríguez F. Marín Iniesta E. Gómez Gómez M.L. Asanza Teruel I. Alcaraz Rojo 《Letters in applied microbiology》1994,19(6):461-465
The enrichment culture method was used to detect and isolate L-aminoacylase-producing bacteria from soil, using N -acetyl-L-alanine as inducer and substrate. Isolated bacterial strains were screened for growth and enzyme activity. Strain BA2 displayed both the highest intracellular L-aminoacylase activity and the most profuse growth. Furthermore, BA2 cells did not show any D-aminoacylase activity. This strain was an obligately aerobic rod-shaped bacterium and stained Gram-negative, and was therefore identified as Pseudomonas. Its morphological and biochemical characteristics corresponded to those of Pseudomonas fluorescens biovar I. 相似文献
423.
Luciana G. Naso Evelina G. Ferrer Nataliya Butenko Isabel Cavaco Luis Lezama Teófilo Rojo Susana B. Etcheverry Patricia A. M. Williams 《Journal of biological inorganic chemistry》2011,16(4):653-668
A new complex of the oxovanadium(IV) cation with the flavolignan silibinin has been synthesized and characterized. Vanadium
compounds show interesting biological and pharmacological properties and some of them display antitumoral actions. Flavonoids
are part of a larger group of antioxidant compounds called polyphenols which may inhibit the proliferation and growth of cancer
cells. The antioxidant and antitumoral effects of silibinin and its oxovanadium(IV) complex were investigated. Silibinin acted
as a very strong antioxidant and its complexation with oxovanadium(IV) improved this behavior. Besides, the generation of
reactive oxygen species (ROS) by this compound was favored in tumoral (UMR106) cells and correlated with the deleterious behavior
in the proliferation of this cell line. Conversely, silibinin did not exert any effect on the proliferation of normal osteoblasts
(MC3T3E1). The cytotoxic action and ROS generation of the oxovanadium(IV) complex was more effective in tumoral cells. This
behavior was not consistent with cleaving DNA of plasmid DNA pA1 because no significant cleaving activity was observed in
both cases. These results suggest that the main deleterious mechanisms may take place through cytotoxic effects more than
genotoxic actions. A comparison with our own findings on the behavior of other flavonoids and their vanadyl(IV) complex has
also been performed. 相似文献