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131.
Sylvia Caiuby Novaes Edgar Teodoro da Cunha 《Visual Anthropology: Published in cooperation with the Commission on Visual Anthropology》2017,30(2):105-146
Although rarely mentioned in English-language texts, Rituais de Festas Borôro has long been acknowledged as a masterpiece of early ethnographic film in the French and Brazilian literature. Shot in 1916 by a Brazilian army officer, Luiz Thomaz Reis, and released in 1917, the film is mainly about the funeral ceremony of the Bororo, an indigenous people of Central Brazil. Here we contrast this work with other ethnographic films of the period and suggest that it has a strong claim to be seen as the first ethnographic documentary in the modern sense of the term. We also consider the political circumstances that led to the filming in this particular form, its status as an ethnographic account of the funeral, and its place in the personal filmography of Luiz Thomaz Reis. 相似文献
132.
Federica Rossi Osvaldo Facini Annalisa Rotondi Silvia Loreti Teodoro Georgiadis 《Trees - Structure and Function》2001,15(8):462-471
Light absorbance and reflectance were measured on representative shrubs of two dominant shrub species (Pistacia lentiscus and Juniperus phoenicea) of coastal Mediterranean macchia ecosystems in Sardinia (Italy). An array of 64 calibrated photodiodes was adopted for both calculations of leaf area index (LAI) and canopy transmittance of photosynthetic active radiation (PAR). PAR and NIR reflectance of canopies having similar LAI allowed us to establish the particular optical properties of each species. Lentisk reflected twice as much as juniper in the 700-1,100 spectral region. Water indexes of the two species were also different in relation to leaf water content and red edge amplitude appeared to be related to chlorophyll content. Epidermis and mesophyll structures of both species are shown. 相似文献
133.
Jessé Henrique Truppel Flavio Otomura Ueslei Teodoro Rubens Massafera Magda Clara Vieira da Costa-Ribeiro Carolina Motter Catarino Luana Dalagrana Maria Eugênia Moreira Costa Ferreira Vanete Thomaz-Soccol 《PloS one》2014,9(4)
In this study, we detected Leishmania (Viannia) braziliensis infection in equids living in endemic regions of cutaneous leishmaniasis. To determine the role of these animals in the Leishmania cycle, we used two approaches: serological and molecular methods. Antibodies to the parasite were assayed using the Enzyme Linked Immunosorbent Assay (ELISA). Blood samples were collected and tested by polymerase chain reaction (PCR), and the positive products were sequenced. The results showed that 11.0% (25/227) of the equids were seropositive for Leishmania sp, and 16.3% (37/227) were PCR positive. Antibodies were detected in 20 horses, 3 donkeys, and 2 mules, and the parasite DNA was detected in 30 horses, 5 donkeys, and 2 mules. Sequencing the amplified DNA revealed 100% similarity with sequences for Viannia complex, corroborating the results of PCR for L. braziliensis. Our results show that equids are infected with L. braziliensis, which could be food sources for phlebotomines in the peridomiciliary environment and consequently play a role in the cutaneous leishmaniasis cycle. 相似文献
134.
In vitro regeneration and cytological characterization of shoots from leaf explants of three accessions of Solanum commersonii 总被引:2,自引:0,他引:2
Teodoro Cardi Vittoria Iannamico Fabrizio D'Ambrosio Edgardo Filippone Paul F. Lurquin 《Plant Cell, Tissue and Organ Culture》1993,34(1):107-114
In vitro culture of explants were used to apply genetic or cell engineering techniques to the sexually incompatible potato relative Solanum commersonii (2n=2x=24) Three accessions of S. commersonii were tested for regeneration from leaf explants using six different protocols. A two step-regeneration procedure gave the best results. Genetic variability for regeneration ability was found between accessions, and between clones within accessions. The accession PI 472834 regenerated at highest frequency. Clones with high regeneration ability were selected. Approximately 60% of regenerated plants were diploids and 40% were tetraploids. A very low frequency of chimeras was found. Leaf shape and chloroplast counts in guard cells were shown to be quick and reliable methods for estimating ploidy levels. Use of the diploid and tetraploid regenerants obtained for potato breeding is discussed.Abbreviations BAP
6-benzylaminopurine
- EBN
Endosperm Balance Number
- GA3
gibberellic acid
- IAA
indole-3-acetic acid
- MS
Murashige and Skoog
- NAA
1-naphthalene-acetic acid
- ZEA
zeatin 相似文献
135.
The early adaptive evolution of calmodulin 总被引:7,自引:0,他引:7
Baba ML; Goodman M; Berger-Cohn J; Demaille JG; Matsuda G 《Molecular biology and evolution》1984,1(6):442-455
Interaction between gene duplication and natural selection in molecular
evolution was investigated utilizing a phylogenetic tree constructed by the
parsimony procedure from amino acid sequences of 50 calmodulin- family
protein members. The 50 sequences, belonging to seven protein lineages
related by gene duplication (calmodulin itself, troponin-C, alkali and
regulatory light chains of myosin, parvalbumin, intestinal calcium-binding
protein, and glial S-100 phenylalanine-rich protein), came from a wide
range of eukaryotic taxa and yielded a denser tree (more branch points
within each lineage) than in earlier studies. Evidence obtained from the
reconstructed pattern of base substitutions and deletions in these
ancestral loci suggests that, during the early history of the family,
selection acted as a transforming force on expressed genes among the
duplicates to encode molecular sites with new or modified functions. In
later stages of descent, however, selection was a conserving force that
preserved the structures of many coadapted functional sites. Each branch of
the family was found to have a unique average tempo of evolutionary change,
apparently regulated through functional constraints. Proteins whose
functions dictate multiple interaction with several other macromolecules
evolved more slowly than those which display fewer protein-protein and
protein-ion interactions, e.g., calmodulin and next troponin-C evolved at
the slowest average rates, whereas parvalbumin evolved at the fastest. The
history of all lineages, however, appears to be characterized by rapid
rates of evolutionary change in earlier periods, followed by slower rates
in more recent periods. A particularly sharp contrast between such fast and
slow rates is found in the evolution of calmodulin, whose rate of change in
earlier eukaryotes was manyfold faster than the average rate over the past
1 billion years. In fact, the amino acid replacements in the nascent
calmodulin lineage occurred at residue positions that in extant metazoans
are largely invariable, lending further support to the Darwinian hypothesis
that natural selection is both a creative and a conserving force in
molecular evolution.
相似文献
136.
Proteolytic enzymes have been used both to modify properties of the cell membrane and to dissociate cells from many tissues including pituitary (4, 5, 12). Exposure of secretory tissues to pronase can alter their secretory response. Thus incubation of pancreatic islets of Langerhans in the presence of low concentrations of pronase increased the subsequent release of insulin in the presence of stimulatory and nonstimulatory glucose concentrations (7). The purpose of the present investigation was to determine whether low concentrations of pronase have the same stimulatory effect on the release of a pituitary hormone, growth hormone. Such an effect on hormone release could be of some importance in view of the development of dissociated cell systems as models for the study of the control of hormone release (4, 5). 相似文献
137.
138.
139.
Lifetime dairy production, reproduction and growth traits of 75 females sired by Holstein, Jersey or Brown Swiss bulls and Holstein-Friesian x Gir dams of 1/2 to 3/4 Holstein-Friesian fractions were compared. The animals were in a single herd under the same management. Milk, fat and protein yields, concentrates fed, reproduction, and weights were recorded throughout the lifetime of the cows. The data were analyzed by least squares techniques under models including the fixed effects of breed of sire, Bos taurus fraction and year of birth. Herd lifes for Holstein, Jersey and Brown Swiss crosses were 6.006 +/- 0.812, 8.129 +/- 0.863 and 7.247 +/- 0.777 years. Milk yields per day of herd life were 7.150 +/- 0.266, 6.757 +/- 0.282 and 6.249 +/- 0.254 kg. Weights of cull cows sold were 458 +/- 15, 415 +/- 15 and 457 +/- 13 kg. Based on these and on previously reported results of the same experiment, intakes of roughage and pasture were estimated from energy requirements. Lifetime expenditures on concentrates, roughages, pastures, milking, reproduction, and heifer rearing were calculated based on mean performance of each breed of sire, as well as on receipts from animals and milk sold (the latter with four sets of prices of protein, fat and carrier). The conclusion was that in systems of artificial female calf rearing and male calf wastage, the Jersey crosses appear to offer important economic benefits to farmers, which would be even greater if payment on milk protein and fat becomes effective. 相似文献
140.
Rodrigo Teodoro Matthias Scheunemann Barbara Wenzel Dan Peters Winnie Deuther-Conrad Peter Brust 《Bioorganic & medicinal chemistry letters》2018,28(9):1471-1475
By structure–activity relationship studies on the tilorone scaffold, the ‘one armed’ substituted dibenzothiophenes and the fluoren-9-ones were identified as the most potential α7 nAChR ligands. While the suitability of dibenzothiophene derivatives as PET tracers is recognized, the potential of fluoren-9-ones is insufficiently investigated. We herein report on a series of fluoren-9-one based derivatives targeting α7 nAChR with compounds 8a and 8c possessing the highest affinity and selectivity. Accordingly, with [18F]8a and [18F]8c we designed and initially evaluated the first fluoren-9-one derived α7 nAChR selective PET ligands. A future application of these radioligands is facilitated by the herein presented successful implementation of fully automated radiosynthesis. 相似文献