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161.
Background
The β1,3-N-acetylglucosaminyltransferase-3 gene (B3GNT3) encodes a member of the B3GNT family that functions as the backbone structure of dimeric sialyl-Lewis A and is involved in L-selectin ligand biosynthesis, lymphocyte homing and lymphocyte trafficking. B3GNT3 has been implicated as an important element in the development of certain cancers. However, the characteristics of B3GNT3 in the development and progression of cancer remain largely unknown. Thus, our study aimed to investigate the expression pattern and the prognostic value of B3GNT3 in patients with early-stage cervical cancer.Methods
The mRNA and protein levels of B3GNT3 expression were examined in eight cervical cancer cell lines and ten paired cervical cancer tumors, using real-time PCR and western blotting, respectively. Immunohistochemistry (IHC) was used to analyze B3GNT3 protein expression in paraffin-embedded tissues from 196 early-stage cervical cancer patients. Statistical analyses were applied to evaluate the association between B3GNT3 expression scores and clinical parameters, as well as patient survival.Results
B3GNT3 expression was significantly upregulated in cervical cancer cell lines and lesions compared with normal cells and adjacent noncancerous cervical tissues. In the 196 cases of tested early-stage cervical cancer samples, the B3GNT3 protein level was positively correlated with high risk TYPES of human papillomavirus (HPV) infection (P = 0.026), FIGO stage (P < 0.001), tumor size (P = 0.025), tumor recurrence (P = 0.004), vital status (P < 0.001), concurrent chemotherapy and radiotherapy (P = 0.016), lymphovascular space involvement (P = 0.003) and most importantly, lymph node metastasis (P = 0.003). Patients with high B3GNT3 expression had a shorter overall survival (OS) and disease-free survival (DFS) compared with those with low expression of this protein. Multivariate analysis suggested that B3GNT3 expression is an independent prognostic indicator for cervical cancer patients.Conclusions
Our study demonstrated that elevated B3GNT3 expression is associated with pelvic lymph node metastasis and poor outcome in early-stage cervical cancer patients. B3GNT3 may be a novel prognostic marker and therapeutic target for the treatment of cervical cancer. 相似文献162.
Yanping Deng Min Yang Feng Xu Qian Zhang Qun Zhao Haitao Yu Defang Li Ge Zhang Aiping Lu Kenka Cho Fukang Teng Peng Wu Linlin Wang Wanying Wu Xuan Liu De-an Guo Baohong Jiang 《PloS one》2015,10(8)
Lack of pharmacological strategies in clinics restricts the patient prognosis with myocardial ischemia/reperfusion (I/R) injury. The aim of this study was to evaluate the cardioprotection of combined salvianolic acid B (SalB) and ginsenoside Rg1 (Rg1) against myocardial I/R injury and further investigate the underlying mechanism. I/R injury was induced by coronary artery ligation for Wistar male rats and hypoxia/reoxygenation injury was induced on H9c2 cells. Firstly, the best ratio between SalB and Rg1was set as 2:5 based on their effects on heart function detected by hemodynamic measurement. Then SalB-Rg1 (2:5) was found to maintain mitochondrial membrane potential and resist apoptosis and necrosis in H9c2 cell with hypoxia/reoxygenation injury. Companying with same dose of SalB or Rg1 only, SalB-Rg1 showed more significant effects on down-regulation of myocardial infarct size, maintenance of myocardium structure, improvement on cardiac function, decrease of cytokine secretion including TNF-α, IL-1β, RANTES and sVCAM-1. Finally, the SalB-Rg1 improved the viability of cardiac myocytes other than cardiac fibroblasts in rats with I/R injury using flow cytometry. Our results revealed that SalB-Rg1 was a promising strategy to prevent myocardial I/R injury. 相似文献
163.
Xuewei Yang Shuang Yang Hongyu Chai Zhaogang Yang Robert J. Lee Weiwei Liao Lesheng Teng 《PloS one》2015,10(8)
We have screened 11 isoquinoline derivatives and α-methylene-γ-butyrolactones using the 3-(4,5-dimethylthi-azol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cytotoxicity assay in HeLa and HEK-293T cells. Compound 2 was identified as potential anticancer agent. To further improve its therapeutic potential, this agent was incorporated into transferrin (Tf)-conjugated liposomes (LPs) for targeted delivery to tumor cells. We have demonstrated Tf-LP-Compound 2 have superior antitumor activity compared to non-targeted controls and the free drug. These data show Tf-LP-Compound 2 to be a promising agent that warrants further evaluation. 相似文献
164.
Ya-Hsu Yang Hao-Wei Teng Yen-Ting Lai Szu-Yuan Li Chih-Ching Lin Albert C. Yang Hsiang-Lin Chan Yi-Hsuan Hsieh Chiao-Fan Lin Fu-Ying Hsu Chih-Kuang Liu Wen-Sheng Liu 《PloS one》2015,10(9)
Objective
Patients with late-onset depression (LOD) have been reported to run a higher risk of subsequent dementia. The present study was conducted to assess whether statins can reduce the risk of dementia in these patients.Methods
We used the data from National Health Insurance of Taiwan during 1996–2009. Standardized Incidence Ratios (SIRs) were calculated for LOD and subsequent dementia. The criteria for LOD diagnoses included age ≥65 years, diagnosis of depression after 65 years of age, at least three service claims, and treatment with antidepressants. The time-dependent Cox proportional hazards model was applied for multivariate analyses. Propensity scores with the one-to-one nearest-neighbor matching model were used to select matching patients for validation studies. Kaplan-Meier curve estimate was used to measure the group of patients with dementia living after diagnosis of LOD.Results
Totally 45,973 patients aged ≥65 years were enrolled. The prevalence of LOD was 12.9% (5,952/45,973). Patients with LOD showed to have a higher incidence of subsequent dementia compared with those without LOD (Odds Ratio: 2.785; 95% CI 2.619–2.958). Among patients with LOD, lipid lowering agent (LLA) users (for at least 3 months) had lower incidence of subsequent dementia than non-users (Hazard Ratio = 0.781, 95% CI 0.685–0.891). Nevertheless, only statins users showed to have reduced risk of dementia (Hazard Ratio = 0.674, 95% CI 0.547–0.832) while other LLAs did not, which was further validated by Kaplan-Meier estimates after we used the propensity scores with the one-to-one nearest-neighbor matching model to control the confounding factors.Conclusions
Statins may reduce the risk of subsequent dementia in patients with LOD. 相似文献165.
Li Jiang Dongxu He Dantong Yang Zhen Chen Qiongxi Pan Aiqin Mao Yanfei Cai Xiyuan Li Hui Xing Mei Shi Yun Chen Iain C. Bruce Teng Wang Linfang Jin Xiaowei Qi Dong Hua Jian Jin Xin Ma 《FEBS letters》2014
To investigate the role of microRNAs in the development of chemoresistance and related epithelial–mesenchymal transition (EMT), we examined the effect of miR-489 in adriamycin (ADM)-resistant human breast cancer cells (MCF-7/ADM). MiR-489 was significantly suppressed in MCF-7/ADM cells compared with chemosensitive parental control MCF-7/WT cells. Forced-expression of miR-489 reversed chemoresistance. Furthermore, Smad3 was identified as the target of miR-489 and is highly expressed in MCF-7/ADM cells. Forced expression of miR-489 both inhibited Smad3 expression and Smad3 related EMT properties. Finally, the interactions between Smad3, miR-489 and EMT were confirmed in chemoresistant tumor xenografts and clinical samples, indicating their potential implication for treatment of chemoresistance. 相似文献
166.
Li Liu Jingang Cui Qinbo Yang Chenglin Jia Minqi Xiong Bingbing Ning Xiaoye Du Peiwei Wang Xintong Yu Li Li Wenjian Wang Yu Chen Teng Zhang 《Biochemical and biophysical research communications》2014
Oxidative stress is mechanistically implicated in the pathogenesis of myocardial injury and the subsequent fibrogenic tissue remodeling. Therapies targeting oxidative stress in the process of myocardial fibrogenesis are still lacking and thus remain as an active research area in myocardial injury management. The current study evaluated the effects of a NADPH oxidase inhibitor, apocynin, on the production of reactive oxygen species and the development of myocardial fibrogenesis in isoproterenol (ISO)-induced myocardial injury mouse model. The results revealed a remarkable effect of apocynin on attenuating the development of myocardial necrotic lesions, inflammation and fibrogenesis. Additionally, the protective effects of apocynin against myocardial injuries were associated with suppressed expression of an array of genes implicated in inflammatory and fibrogenic responses. Our study thus provided for the first time the histopathological and molecular evidence supporting the therapeutic value of apocynin against the development of myocardial injuries, in particular, myocardial fibrogenesis, which will benefit the mechanism-based drug development targeting oxidative stress in preventing and/or treating related myocardial disorders. 相似文献
167.
Shao-Xiang Teng Zhong-Hua Tong Wen-Wei Li Shu-Guang Wang Guo-Ping Sheng Xian-Yang Shi Xian-Wei Liu Han-Qing Yu 《Applied microbiology and biotechnology》2010,87(6):2365-2372
Fermentative hydrogen production, as a process for clean energy recovery from organic wastewater, is limited by its low hydrogen
yield due to incomplete conversion of substrates, with most of the fermentation products being volatile fatty acids (VFAs).
Thus, further recovery of the energy from VFAs is expected. In this work, microbial fuel cell (MFC) was applied to recover
energy in the form of electricity from mixed VFAs of acetate, propionate, and butyrate. Response surface methodology was adopted
to investigate the relative contribution and possible interactions of the three components of VFAs. A stable electricity generation
was demonstrated in MFCs after the enrichment of electrochemically active bacteria. Analysis showed that power density was
more sensitive to the composition of mixed VFAs than coulombic efficiency. The electricity generation could mainly be attributed
to the portion of acetate and propionate. However, the two components showed an antagonistic effect when propionate exceeded
19%, causing a decrease in coulombic efficiency. Butyrate was found to exert a negative impact on both power density and coulombic
efficiency. Denaturing gradient gel electrophoresis profiles revealed the enrichment of electrochemically active bacteria
from the inoculum sludge. Proteobacteria (Beta-, Delta-) and Bacteroidetes were predominant in all VFA-fed MFCs. Shifts in
bacterial community structures were observed when different compositions of VFA mixtures were used as the electron donor.
The overall electron recovery efficiency may be increased from 15.7% to 27.4% if fermentative hydrogen production and MFC
processes are integrated. 相似文献
168.
J.‐M. Fan J. Luo G.‐P. Zhang L. Chen M. Teng M.‐F. Yang L. Wang C.‐Q. Wang 《Letters in applied microbiology》2010,51(1):11-17
Aims: Identification and characterization of Japanese encephalitis virus (JEV) envelope protein gene from swine. Methods and Results: Genomic RNA was separated from JEV isolated strain Henan‐09‐03, and used as templates for cDNA synthesis of E gene. The cDNA of E gene was amplified by RT‐PCR and cloned into the pMD19‐T‐Vector and confirmed by sequencing. The cloned gene was then subcloned into the pET‐32a and was introduced into Escherichia coli BL21 (DE3) for expression. The E protein was purified by Ni chelating column‐based affinity chromatography. The molecular weight of expressed protein was about 50 kDa. Compared with the published sequence of SA14 ( AF495589 ), the homology of the nucleotide sequence was 98% and the seven mutations resulting in amino acid substitutions at Leu 36 Ser, Leu107 Val, Ala167 Thr, Asn 230 Ser, Leu 340 Pro, Asn 430 Ile, Phe 448 Leu. Phylogenetic analysis of the E sequence of isolated strain classified it within genotype III of the JEV. The result of Western blotting indicated that the antigenicity of the protein was specific. Conclusions: The stable expression of the protein and the analysis of its antigenic specificity provide the foundation for developing the ELISA early stage diagnosis kit. Significance and Impact of the Study: As coating antigen, the recombinant E protein served a good source in the indirect ELISA method for the detection of JEV antibody. 相似文献
169.
Shen-Long Howng Chi-Ching Hwang Chia-Yi Hsu Meng-Yu Hsu Chun-Yen Teng Chia-Hua Chou Mei-Feng Lee Chia-Hung Wu Shean-Jaw Chiou Ann-Shung Lieu Joon-Khim Loh Chia-Ning Yang Chan-Shing Lin Yi-Ren Hong 《Molecular and cellular biochemistry》2010,339(1-2):23-33
The specificity and regulation of GSK3β are thought to involve in the docking interactions at core kinase domain because of the particular amino acid residues. Recent X-ray diffraction studies illuminated the relative binding residues on AxinGID and FRATtide for GSK3β docking and appeared that GSK3β Val267Gly (V267G) and Tyr288Phe (Y288F) could distinguish the direct interaction between AxinGID and FRATtide. In order to explore the mode that involved the binding of GSKIP to GSK3β and compare it with that of AxinGID and FRATtide, we pinpointed the binding sites of GSKIP to GSK3β through the single-point mutation of four corresponding sites within GSK3β (residues 260–300) as scaffold-binding region I (designated SBR-I260–300). Our data showed that these three binding proteins shared similar binding sites on GSK3β. We also found that the binding of GSK3β V267G mutant to GSKIP and AxinGID, but not that of Y288F mutant (effect on FRATtide), was affected. Further, based on the simulation data, the electron-density map of GSKIPtide bore closer similarity to the map AxinGID than to that of FRATtide. Interestingly, many C-terminal helix region point-mutants of GSK3β L359P, F362A, E366K, and L367P were able to eliminate the binding with FRATtide, but not AxinGID or GSKIP. In addition, CABYR exhibited a unique mode in binding to C-terminal helix region of GSK3β. Taken together, our data revealed that in addition to the core kinase domain, SBR-I260–300, another novel C-terminus helix region, designated SBR-II339–383, also appeared to participate in the recognition and specificity of GSK3β in binding to other specific proteins. 相似文献
170.