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排序方式: 共有139条查询结果,搜索用时 234 毫秒
71.
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73.
Midori Okabayashi Nobutaka Takahashi Teiichi Fukami 《Bioscience, biotechnology, and biochemistry》2013,77(5):877-879
Dehydrodicaffeic acid dilactone (DDACD) was found in a cultured mushroom by screening for catechol-O-methyltransferase inhibitors. The enzyme which converts two molecules of caffeic acid to DDCAD has been extracted from the mushroom and purified and the enzyme reaction has been studied. It was markedly inhibited by reducing agents, such as NADPH, NADH, glutathione and ascorbic acid but stimulated by Fe3+, Fe2+, Co2+, Ni2+, Cu2+, Cu+ and Zn2+ ions. Sodium diethyldithiocarbamate and sodium cyanide known to be copper chelating agents inactivated the enzyme, but activity was restored by addition of Cu2+ or Cu+. Although the enzymic reaction did not occur under anaerobic conditions, 18O-oxygen was not incorporated into DDCAD. o-Diphenol oxidase catalyzed DDCAD formation from caffeic acid and the DDCAD-forming enzyme catalyzed the formation of DOPAchrome from DOPA. Thus, the DDCAD-forming enzyme is a type of o-diphenol oxidase. Peroxidase and hydrogen peroxide produced DDCAD from caffeic acid.On the other hand, DDCAD was non-enzymatically synthesized from caffeic acid in the presence of CuCl2 in 64% yield. In both enzymic and non-enzymic syntheses, both (+)- DDCAD and (?)-DDCAD were produced. 相似文献
74.
Michiko Yamashita Teiichi Eto Yukio Okazawa Masao Fujimaki Yosito Sakurai 《Bioscience, biotechnology, and biochemistry》2013,77(7):900-906
By administering 2 mg/day of cortisone acetate to adrenalectomized rats, the hepatic threonine dehydratase activity of these rats increased 5 times as much as that of the control. By administering 5 IU/day of ACTH to hypophysectomized rats, both the hepatic threonine dehydratase activity and the adrenal glucose-6-phosphate dehydrogenase activity increased 3 times and 7 times as much as that of the control group, respectively. The effects of excess feeding of lysine or threonine on the increase of the dehydratase activity by the adminitration of cortisone to the adrenalectomized rats and the administration of ACTH to the hypophysetomized rats were negative. When the intact rats were fed on lysine and/or threonine excess diet, the amount of glucocorticoid secretion as measured by the adrenal glucose-6-phosphate dehydrogenase activity increased and the hepatic threonine dehydratase activity increased accordingly. A linear relationship was found between these two activities and no significant deviation from the relationship due to the difference in diet composition was observed. A mechanism was proposed, based on these results, explaining the fact that the hepatic threonine dehydratase activity increased when rats were fed on lysine or threonine excess diet. 相似文献
75.
Akiko Motoyoshi-Yamashiro Mizuho Tamura Mitsuaki Moriyama Katsura Takano Kenji Kawabe Hidemitsu Nakajima Ritsuko Katoh-Semba Teiichi Furuichi Yoichi Nakamura 《Neurochemistry international》2013
Amphotericin B (AmB) is a polyene antibiotic and reported to be one of a few reagents having therapeutic effects on prion diseases, such as the delay in the appearing of the clinical signs and the prolongation of the survival time. In prion diseases, glial cells have been suggested to play important roles by proliferating and producing various factors such as nitric oxide, proinflammatory cytokines, and neurotrophic factors. However, the therapeutic mechanism of AmB on prion diseases remains elusive. We have previously reported that AmB changed the expression of neurotoxic and neurotrophic factors in microglia (Motoyoshi et al., 2008, Neurochem. Int. 52, 1290–1296). In the present study, we examined the effects of AmB on cellular functions of rat cultured astrocytes. We found that AmB could activate astrocytes to produce nitric oxide via inducible nitric oxide synthase induction. AmB also induced mRNA expression of interleukin-1β and tumor necrosis factor-α, and productions of their proteins in astrocytes. Moreover, AmB changed levels of neurotrophic factor mRNAs and proteins. Among three neurotrophic factors examined here, neurotrophin-3 mRNA expression and its protein production in the cells were down-regulated by AmB stimulation. On the other hand, AmB significantly enhanced the amounts of glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor proteins in the cells and the medium. These results suggest that AmB might show therapeutic effects on prion diseases by controlling the expression and production of such mediators in astrocytes. 相似文献
76.
Keiichi Ito Taro Date Masahiro Ikegami Kenichi Hongo Masami Fujisaki Daisuke Katoh Takuya Yoshino Ryuko Anzawa Tomohisa Nagoshi Seigo Yamashita Keiichi Inada Seiichiro Matsuo Teiichi Yamane Michihiro Yoshimura 《PloS one》2013,8(6)
Objective
Thrombin, the final coagulation product of the coagulation cascade, has been demonstrated to have many physiological effects, including pro-fibrotic actions via protease-activated receptor (PAR)-1. Recent investigations have demonstrated that activation of the cardiac local coagulation system was associated with atrial fibrillation. However, the distribution of thrombin in the heart, especially difference between the atria and the ventricle, remains to be clarified. We herein investigated the expression of thrombin and other related proteins, as well as tissue fibrosis, in the human left atria and left ventricle.Methods
We examined the expression of thrombin and other related molecules in the autopsied hearts of patients with and without atrial fibrillation. An immunohistochemical analysis was performed in the left atria and the left ventricle.Results
The thrombin was immunohistologically detected in both the left atria and the left ventricles. Other than in the myocardium, the expression of thrombin was observed in the endocardium and the subendocardium of the left atrium. Thrombin was more highly expressed in the left atrium compared to the left ventricle, which was concomitant with more tissue fibrosis and inflammation, as detected by CD68 expression, in the left atrium. We also confirmed the expression of prothrombin in the left atrium. The expression of PAR-1 was observed in the endocardium, subendocardium and myocardium in the left atrium. In patients with atrial fibrillation, strong thrombin expression was observed in the left atrium.Conclusions
The strong expression levels of thrombin, prothrombin and PAR-1 were demonstrated in the atrial tissues of human autopsied hearts. 相似文献77.
Ohnishi T Ohba H Seo KC Im J Sato Y Iwayama Y Furuichi T Chung SK Yoshikawa T 《The Journal of biological chemistry》2007,282(1):637-646
Lithium is used in the clinical treatment of bipolar disorder, a disease where patients suffer mood swings between mania and depression. Although the mode of action of lithium remains elusive, a putative primary target is thought to be inositol monophosphatase (IMPase) activity. Two IMPase genes have been identified in mammals, the well characterized myo-inositol monophosphatase 1 (IMPA1) and myo-inositol monophosphatase 2 (IMPA2). Several lines of genetic evidence have implicated IMPA2 in the pathogenesis of not only bipolar disorder but also schizophrenia and febrile seizures. However, little is known about the protein, although it is predicted to have lithium-inhibitable IMPase activity based on its homology to IMPA1. Here we present the first biochemical study comparing the enzyme activity of IMPA2 to that of IMPA1. We demonstrate that in vivo, IMPA2 forms homodimers but no heterodimers with IMPA1. Recombinant IMPA2 exhibits IMPase activity, although maximal activity requires higher concentrations of magnesium and a higher pH. IMPA2 shows significantly lower activity toward myo-inositol monophosphate than IMPA1. We therefore screened for additional substrates that could be more efficiently dephosphorylated by IMPA2, but failed to find any. Importantly, when using myo-inositol monophosphate as a substrate, the IMPase activity of IMPA2 was inhibited at high lithium and restricted magnesium concentrations. This kinetics distinguishes it from IMPA1. We also observed a characteristic pattern of differential expression between IMPA1 and IMPA2 in a selection of tissues including the brain, small intestine, and kidney. These data suggest that IMPA2 has a separate function in vivo from that of IMPA1. 相似文献
78.
Aruga J Yoshikawa F Nozaki Y Sakaki Y Toyoda A Furuichi T 《Journal of neurochemistry》2007,102(5):1533-1547
Opalin is a transmembrane protein detected specifically in mammalian oligodendrocytes. Opalin homologs are found only in mammals and not in the genome sequences of other animal classes. We first determined the nucleotide sequences of Opalin orthologs and their flanking regions derived from four prosimians, a group of primitive primates. A global comparison revealed that an evolutionarily conserved region exists in the first intron of Opalin. When the conserved domain was assayed for its enhancer activity in transgenic mice, oligodendrocyte-directed expression was observed. In an oligodendroglial cell line, Oli-neu, the conserved domain showed oligodendrocyte-directed expression. The conserved domain is composed of eight subdomains, some of which contain binding sites for Myt1 and cAMP-response element binding protein (CREB). Deletion analysis and cotransfection experiments revealed that the subdomains have critical roles in Opalin gene expression. Over-expression of Myt1, treatment of the cell with leukemia inhibitory factor (LIF), and cAMP analog (CREB activator) enhanced the expression of endogenous Opalin in Oli-neu cells and activated the oligodendrocyte enhancer. These results suggest that LIF, cAMP signaling cascades and Myt1 play significant roles in the differentiation of oligodendrocytes through their action on the Opalin oligodendrocyte enhancer. 相似文献
79.
Takamasa Tobimatsu Tsuneo Nishiki Masaya Morimoto Ryou Miyata Tetsuo Toraya 《Archives of microbiology》2009,191(3):199-206
Coenzyme B12-dependent diol and glycerol dehydratases are isofunctional enzymes, which catalyze dehydration of 1, 2-diols to produce corresponding
aldehydes. Although the two types of dehydratases have high sequence homology, glycerol dehydratase is a soluble cytosolic
enzyme, whereas diol dehydratase is a low-solubility enzyme associated with carboxysome-like polyhedral organelles. Since
both the N-terminal 20 and 16 amino acid residues of the β and γ subunits, respectively, are indispensable for the low solubility
of diol dehydratase, we constructed glycerol dehydratase-based chimeric enzymes which carried N-terminal portions of the β
and γ subunits of diol dehydratase in the corresponding subunits of glycerol dehydratase. Addition of the diol dehydratase-specific
N-terminal 34 and 33 amino acid residues of the β and γ subunits, respectively, was not enough to lower the solubility of
glycerol dehydratase. A chimeric enzyme which carries the low homology region (residues 35–60) of the diol dehydratase β subunit
in addition to the diol dehydratase-specific extra-regions of β and γ subunits showed low solubility comparable to diol dehydratase,
although its hydropathy plot does not show any prominent hydrophobic peaks in these regions. It was thus concluded that short
N-terminal sequences are sufficient to change the solubility of the enzyme. 相似文献
80.
W Hueb BJ Gersh PC Rezende CL Garzillo EG Lima RD Vieira RM Garcia D Favarato CA Segre AC Pereira PR Soares E Ribeiro P Lemos MA Perin CC Strunz LA Dallan FB Jatene NA Stolf AC Hueb R Dias FA Gaiotto LM Costa FT Oikawa RM Melo CV Serrano LF Avila AV Villa JR Parga Filho C Nomura JA Ramires R Kalil Filho 《BMC cardiovascular disorders》2012,12(1):65
ABSTRACT: BACKGROUND: Although the release of cardiac biomarkers after percutaneous (PCI) or surgical revascularization (CABG) is common, its prognostic significance is not known. Questions remain about the mechanisms and degree of correlation between the release, the volume of myocardial tissue loss, and the long-term significance. Delayed-enhancement of cardiac magnetic resonance (CMR) consistently quantifies areas of irreversible myocardial injury. To investigate the quantitative relationship between irreversible injury and cardiac biomarkers, we will evaluate the extent of irreversible injury in patients undergoing PCI and CABG and relate it to postprocedural modifications in cardiac biomarkers and long-term prognosis. METHODS: The study will include 150 patients with multivessel coronary artery disease (CAD) with left ventricle ejection fraction (LVEF) and a formal indication for CABG; 50 patients will undergo CABG with cardiopulmonary bypass (CPB); 50 patients with the same arterial and ventricular condition indicated for myocardial revascularization will undergo CABG without CPB; and another 50 patients with CAD and preserved ventricular function will undergo PCI using stents. All patients will undergo CMR before and after surgery or PCI. We will also evaluate the release of cardiac markers of necrosis immediately before and after each procedure. Primary outcome considered is overall death in a 5-year follow-up. Secondary outcomes are levels of CK-MB isoenzyme and I-Troponin in association with presence of myocardial fibrosis and systolic left ventricle dysfunction assessed by CMR. DISCUSSION: The MASS-V Trial aims to establish reliable values for parameters of enzyme markers of myocardial necrosis in the absence of manifest myocardial infarction after mechanical interventions. The establishments of these indices have diagnostic value and clinical prognosis and therefore require relevant and different therapeutic measures. In daily practice, the inappropriate use of these necrosis markers has led to misdiagnosis and therefore wrong treatment. The appearance of a more sensitive tool such as CMR provides an unprecedented diagnostic accuracy of myocardial damage when correlated with necrosis enzyme markers. We aim to correlate laboratory data with imaging, thereby establishing more refined data on the presence or absence of irreversible myocardial injury after the procedure, either percutaneous or surgical, and this, with or without the use of cardiopulmonary bypass. 相似文献