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排序方式: 共有247条查询结果,搜索用时 31 毫秒
101.
Mikko Tiusanen Tea Huotari Paul D. N. Hebert Tommi Andersson Ashley Asmus Joël Bêty Emma Davis Jennifer Gale Bess Hardwick David Hik Christian Krner Richard B. Lanctot Maarten J. J. E. Loonen Rauni Partanen Karissa Reischke Sarah T. Saalfeld Fanny Senez‐Gagnon Paul A. Smith Jn ulavík Ilkka Syvnper Christine Urbanowicz Sian Williams Paul Woodard Yulia Zaika Tomas Roslin 《Molecular ecology》2019,28(2):318-335
102.
Tea Omanovic Kolaric Tomislav Kizivat Vjera Mihaljevic Milorad Zjalic Ines Bilic-Curcic Lucija Kuna Robert Smolic Aleksandar Vcev George Y. Wu Martina Smolic 《Current issues in molecular biology》2022,44(8):3465
(1) Background: With the aging of the population and polypharmacy encountered in the elderly, drug-induced steatosis (DIS) has become frequent cause of non-alcoholic steatosis (NAS). Indeed, NAS and DIS may co-exist, making the ability to distinguish between the entities ever more important. The aim of our study was to study cell culture models of NAS and DIS and determine the effects of liraglutide (LIRA) in those models. (2) Methods: Huh7 cells were treated with oleic acid (OA), or amiodarone (AMD) to establish models of NAS and DIS, respectively. Cells were treated with LIRA and cell viability was assessed by MTT, lipid accumulation by Oil-Red-O staining and triglyceride assay, and intracellular signals involved in hepatosteatosis were quantitated by RT-PCR. (3) Results: After exposure to various OA and AMD concentrations, those that achieved 80% of cells viabilities were used in further experiments to establish NAS and DIS models using 0.5 mM OA and 20 µM AMD, respectively. In both models, LIRA increased cell viability (p < 0.01). Lipid accumulation was increased in both models, with microsteatotic pattern in DIS, and macrosteatotic pattern in NAS which corresponds to greater triglyceride accumulation in latter. LIRA ameliorated these changes (p < 0.001), and downregulated expression of lipogenic ACSL1, PPARγ, and SREBP-1c pathways in the liver (p < 0.01) (4) Conclusions: LIRA ameliorates hepatocyte steatosis in Huh7 cell culture models of NAS and DIS. 相似文献
103.
Joy S. Tea Albert Cespedes Daniel Dawson Utpal Banerjee Gerald B. Call 《Journal of visualized experiments : JoVE》2014,(93)
The Drosophila melanogaster eye disc is a powerful system that can be used to study many different biological processes. It contains approximately 800 separate eye units, termed ommatidia1. Each ommatidium contains eight neuronal photoreceptors that develop from undifferentiated cells following the passage of the morphogenetic furrow in the third larval instar2. Following the sequential differentiation of the photoreceptors, non-neuronal cells develop, including cone and pigment cells, along with mechanosensory bristle cells3. Final differentiation processes, including the structured arrangement of all the ommatidial cell types, programmed cell death of undifferentiated cell types and rhodopsin expression, occurs through the pupal phase4-7. This technique focuses on manipulating the pupal eye disc, providing insight and instruction on how to dissect the eye disc during the pupal phase, which is inherently more difficult to perform than the commonly dissected third instar eye disc. This technique also provides details on immunostaining to allow the visualization of various proteins and other cell components. 相似文献
104.
Ildiko Lingvay Kirstine Brown-Frandsen Helen M. Colhoun John Deanfield Scott S. Emerson Sille Esbjerg Søren Hardt-Lindberg G. Kees Hovingh Steven E. Kahn Robert F. Kushner A. Michael Lincoff Steven P. Marso Tea Monk Fries Jorge Plutzky Donna H. Ryan the SELECT Study Group 《Obesity (Silver Spring, Md.)》2023,31(1):111-122
105.
We used simulated data to investigate a number of properties of maximum-
likelihood (ML) phylogenetic tree estimation for the case of four taxa.
Simulated data were generated under a broad range of conditions, including
wide variation in branch lengths, differences in the ratio of transition
and transversion substitutions, and the absence of presence of
gamma-distributed site-to-site rate variation. Data were analyzed in the ML
framework with two different substitution models, and we compared the
ability of the two models to reconstruct the correct topology. Although
both models were inconsistent for some branch-length combinations in the
presence of site-to-site variation, the models were efficient predictors of
topology under most simulation conditions. We also examined the performance
of the likelihood ratio (LR) test for significant positive interior branch
length. This test was found to be misleading under many simulation
conditions, rejecting too often under some simulation conditions. Under the
null hypothesis of zero length internal branch, LR statistics are assumed
to be asymptotically distributed chi 2(1); with limited data, the
distribution of LR statistics under the null hypothesis varies from chi
2(1).
相似文献
106.
Tian-Yi?Chi George?G?Chen Lok-Kee?Ho Paul?BS?LaiEmail author 《Cancer cell international》2005,5(1):27
p53 is important in the development of hepatocellular carcinoma (HCC) and in therapeutic approaches, but the mechanism whereby
it inhibits HCC growth is still unclear. The aim of the present study was to establish a HCC cell system in which p53 levels
can be regulated. Full-length wild-type p53 cDNA obtained by PCR was cloned into a retroviral response vector controlled by
the tetracycline responsive element (RevTRE-p53). The regulatory vectors RevTet-Off and RevTRE-p53 were transfected into a
packaging cell line, PT67. Hep3B cells in which the p53 gene was deleted were infected with RevTet-Off viral particles from
the PT67. Three G418-resistant cell clones with high luciferase expression and low background were infected with RevTRE-p53.
By screening dozens of RevTRE-p53-infected clones with hygromycin we identified the one with the highest expression of p53
and the lowest background after doxycycline treatment. The results showed that p53 expression in this cell clone could be
simply turned on or off by removing or adding doxycycline. Furthermore, it was found that the level of p53 protein was negatively
and sensitively related to the doxycycline concentration. In conclusion, we have established a HCC cell line in which p53
expression can be switched on or off and regulated in a dose- and time-dependent manner. 相似文献
107.
Deblois D Tea BS Beaudry D Hamet P 《Canadian journal of physiology and pharmacology》2005,83(1):29-41
Cell growth and survival are potential therapeutic targets for the control of complications associated with hypertension. In most cardiovascular disorders, cardiac fibroblasts and large-vessel smooth muscle cells can replicate and thus contribute to the disease. We propose that cardiovascular hyperplasia may be reversed via therapeutic apoptosis induction with drugs that are safe and already used in the clinic. We first reported that, irrespective of the drug class, those drugs that are able to induce regression of cardiovascular hypertrophy are also able to reverse cardiovascular hyperplasia via apoptosis. Drugs active in this regard include inhibitors of the renin-angiotensin system, calcium channel blockers, and beta-blockers. Moreover, the effects of these drugs on cell survival is not merely secondary to blood pressure reduction. Therapeutic apoptosis in the cardiovascular system of the spontaneously hypertensive rat is characterized by a rapid and transient onset following initiation of antihypertensive treatment. Herein, the induction and termination of therapeutic apoptosis during drug treatment of hypertension will be briefly reviewed and supported by novel data suggesting that reversal of cardiovascular hyperplasia is associated with reduced cell growth and a resistance to further induction of therapeutic apoptosis, as shown in spontaneously hypertensive rats receiving an intermittent regime of nifedipine therapy. We propose that the presence of a cell subpopulation with defective cell cycle regulation may determine organ susceptibility to undergo therapeutic apoptosis. 相似文献
108.
Turner ML Cockerell EJ Brereton HM Badenoch PR Tea M Coster DJ Williams KA 《International journal for parasitology》2005,35(9):981-990
Acanthamoeba species are ubiquitous soil and freshwater protozoa that have been associated with infections of the human brain, skin, lungs and eyes. Our aim was to develop specific antibodies to aid in rapid and specific diagnosis of clinically important isolates. Mice were variously immunised with live mixtures of Acanthamoeba castellanii strain 112 (AC112) trophozoites and cysts, or with sonicated, formalin-fixed or heat-treated trophozoites, or with a trophozoite membrane preparation. Eight hybridoma cell lines secreting monoclonal antibodies reactive with A. castellanii epitopes were generated. Seven of the new antibodies (designated AMEC1-3 and MTAC1-4) were isotyped as IgMkappa and one (MTAC5) as IgG1kappa. All of the novel antibodies bound to AC112 cysts, and MTAC4 and MTAC5 also bound to trophozoites as measured by flow cytometry on unfixed cells. Single chain antibody fragments that retained parental antibody binding characteristics were engineered from three of the hybridomas (AMEC1, MTAC3 and MTAC4). Four monoclonal antibodies (AMEC1, AMEC3, MTAC1, MTAC3) bound reliably to unfixed cysts of clinical isolates of A. castellanii (two strains) and Acanthamoeba polyphaga (two strains), belonging to Pussard-Pons morphological group II, and to Acanthamoeba lenticulata and Acanthamoeba culbertsoni, belonging to Pussard-Pons morphological group III. None of the antibodies bound to cysts or trophozoites of the environmental group I species, Acanthamoeba tubiashi. Antibodies AMEC1, MTAC3, MTAC4 and MTAC5 reacted with buffered formalin-fixed AC112 by immunohistochemistry, and also stained Acanthamoeba in sections of infected rat cornea and buffered formalin-fixed, paraffin-embedded infected human cornea. These antibodies may be useful in diagnosing pathogenic Acanthamoeba species in clinical specimens, provided that cysts are present. 相似文献
109.
An attachment tip and pili-like structures in insect- and plant-pathogenic spiroplasmas of the class Mollicutes 总被引:1,自引:0,他引:1
Ultrastructural studies using scanning electron microscopy (SEM), negative-staining transmission electron microscopy (TEM), and thin-sectioning TEM on four species of Spiroplasma, in vitro and/or in vivo, indicated that their helices commonly possess one tapered end (tip structure) and one blunt or round end. These tip structures appeared morphologically different from the rest of the helix, exhibiting an electron-dense conical or rod-shaped core. In thin sections of the midgut of the leafhopper Dalbulus elimatus, the tip structures of Spiroplasma kunkelii in the midgut lumen were mostly aligned between microvilli, perpendicular to the apical plasma membrane of epithelial cells. These tip structures appeared frequently attached or closely apposed to the plasma membrane, in which cup-shaped invaginations close to the tips were observed. Pleomorphic forms of spiroplasma, enclosed in membranous vesicles, were found in the cytoplasm of the midgut epithelial cells. These findings suggest that the tip structure may be involved in the orientation and attachment of spiroplasma helices in relation to their host cells, and thus may be functionally comparable to the attachment organelle of mycoplasmas. Additionally, pili-like structures were observed by negative-staining TEM on the surface of Spiroplasma melliferum, and in thin sections of S. kunkelii infecting the leafhopper vector Dalbulus gelbus.
Abbreviations
CSS
Corn stunt spiroplasma
-
SEM
Scanning electron microscopy
-
TBS
Tris-buffered saline
-
TEM
Transmission electron microscopy 相似文献
110.