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排序方式: 共有999条查询结果,搜索用时 15 毫秒
991.
Larissa Tavares Cyrino Alexandra Paiva Araújo Paulo Pinto Joazeiro Cristina Pontes Vicente Selma Giorgio 《Tissue & cell》2012,44(6):401-408
Autophagy is the primary mechanism of degradation of cellular proteins and at least two functions can be attributed to this biological phenomenon: increased nutrient supply via recycling of the products of autophagy under nutrient starvation; and antimicrobial response involved in the innate immune system. Many microorganisms induce host cell autophagy and it has been proposed as a pathway by which parasites compete with the host cell for limited resources. In this report we provide evidence that the intracellular parasite Leishmania amazonensis induces autophagy in macrophages. Using western blotting, the LC3II protein, a marker of autophagosomes, was detected in cell cultures with a high infection index. Macrophages infected with L. amazonensis were examined by transmission electronic microscopy, which revealed enlarged myelin-like structures typical late autophagosome and autolysosome. Other evidence indicating autophagy was Lysotracker red dye uptake by the macrophages. Autophagy also occurs in the leishmaniasis skin lesions of BALB/c mice, detected by immunohistochemistry with anti-LC3II antibody. In this study, autophagy inhibitor 3-methyladenine (3MA) reduced the infection index, while autophagy inductors, such as rapamycin or starvation, did not alter the infection index in cultivated macrophages, suggesting that one aspect of the role of autophagy could be the provision of nutritive support to the parasite. 相似文献
992.
993.
Poliana de Oliveira Figueiredo André Tavares da Silva-Fernandes Bruno Eduardo Fernandes Mota Galileu Barbosa Costa Iara Apolinário Borges Paulo César Peregrino Ferreira J?natas Santos Abrah?o Erika Martins Braga Erna Geessien Kroon Giliane de Souza Trindade 《Memórias do Instituto Oswaldo Cruz》2015,110(6):804-808
Vaccinia virus naturally circulates in Brazil and is the causative agent of a
zoonotic disease known as bovine vaccinia (BV). We retrospectively evaluated two
populations from the Amazon and Southeast Regions. BV outbreaks had not been reported
in these regions before sample collection. Neutralising antibodies were found in 13
individuals (n = 132) with titres ranging from 100 ≥ 6,400 neutralising units/mL.
Univariate analysis identified age and vaccination as statistically significant risk
factors in individuals from the Southeast Region. The absence of detectable
antibodies in vaccinated individuals raises questions about the protection of
smallpox vaccine years after vaccination and reinforces the need for surveillance of
Orthopoxvirus in Brazilian populations without evidence of previous outbreaks. 相似文献
994.
Wanderson M. Silva Cassiana S. Sousa Leticia C. Oliveira Siomar C. Soares Gustavo F.M.H. Souza Guilherme C. Tavares Cristiana P. Resende Edson L. Folador Felipe L. Pereira Henrique Figueiredo Vasco Azevedo 《Microbial biotechnology》2019,12(2):265-274
Lactococcus lactis is a bacteria with high biotechnological potential, where is frequently used in the amino acid production and production of fermented dairy products, as well as drug delivery systems and mucosal vaccine vector. The knowledge of a functional core proteome is important extremely for both fundamental understanding of cell functions and for synthetic biology applications. In this study, we characterized the L. lacits proteome from proteomic analysis of four biotechnological strains L. lactis: L. lactis subsp. lactis NCDO2118, L. lactis subsp. lactis IL1403, L. lactis subsp. cremoris NZ9000 and L. lactis subsp. cremoris MG1363. Our label-free quantitative proteomic analysis of the whole bacterial lysates from each strains resulted in the characterization of the L. lactis core proteome that was composed by 586 proteins, which might contribute to resistance of this bacterium to different stress conditions as well as involved in the probiotic characteristic of L. lactis. Kegg enrichment analysis shows that ribosome, metabolic pathways, pyruvate metabolism and microbial metabolism in diverse environments were the most enriched. According to our quantitative proteomic analysis, proteins related to translation process were the more abundant in the core proteome, which represent an important step in the synthetic biology. In addition, we identified a subset of conserved proteins that are exclusive of the L. lactis subsp. cremoris or L. lactis subsp. lactis, which some are related to metabolic pathway exclusive. Regarding specific proteome of NCDO2118, we detected ‘strain-specific proteins’. Finally, proteogenomics analysis allows the identification of proteins, which were not previously annotated in IL1403 and MG1363. The results obtained in this study allowed to increase our knowledge about the biology of L. lactis, which contributes to the implementation of strategies that make it possible to increase the biotechnological potential of this bacterium. 相似文献
995.
Junio Tavares Amaro Adeney de Freitas Bueno Pedro Manuel Oliveira Janeiro Neves Débora Mello da Silva Aline Pomari-Fernandes Bruna Magda Favetti 《Revista Brasileira de Entomologia》2018,62(3):195-197
The selectivity of five entomopathogens and a chemical insecticide (positive control) to pupae and adults of the egg parasitoid Telenomus remus (Nixon) was evaluated in the laboratory under controlled environmental conditions according to protocols established by the International Organization for Biological Control. Baculovirus anticarsia (Baculovirus AEE®), Bacillus thuringiensis var. kurstaki (Thuricide®), Bacillus thuringiensis var. aizawai (Agree®), Beauveria bassiana (Boveril®), Metarhizium anisopliae (Metarril®) and Trichoderma harzianum (Trichodermil®) are harmless to T. remus pupae, and adults. Thus, our results suggest that the insect control strategies applied here are compatible since entomopathogens were classified as harmless to T. remus in most examined cases and therefore facilitate a joint application to control different pests. Bacillus thuringiensis var. kurstaki (Dipel®), despite being classified as slightly harmful in some of the evaluations, can still be considered compatible for use together with T. remus, especially when compared with chemical insecticides such as chlorpyrifos that might be considered harmful to the parasitoid survival. 相似文献
996.
Pagels Fernando Amaro Helena M. Tavares Tânia G. Casal Susana Malcata F. Xavier Sousa-Pinto Isabel Guedes A. Catarina 《Journal of applied phycology》2021,33(3):1379-1388
Journal of Applied Phycology - Microalgae have shown a great potential as a source of bioactive compounds, such lipidic compounds as fatty acids and carotenoids, with recognized benefit for human... 相似文献
997.
Herlânder Azevedo Alberto Dias Rui Manuel Tavares 《Plant Cell, Tissue and Organ Culture》2008,93(1):115-121
We report the establishment of a Pinus pinaster (Ait.) cell suspension culture in a modified MS medium supplemented with 2 mg ml−1 2,4-D and 1 mg ml−1 BA. Calli were obtained from seedling root segments and established a friable isodiametric cell suspension, suitable for in vitro studies
of maritime pine at the cellular level. Growth (dry weight), cell viability, pH, and nutrient consumption: carbon source (sucrose,
fructose and glucose), nitrogen source (ammonia and nitrate) and phosphate were monitored over 24 h. Suspension cells exhibited
a 15-day exponential growth stage, during which a biphasic consumption profile was observed for all nutrients. Phosphate was
the first limiting nutrient and preferable consumption was observed for glucose over fructose and nitrate over ammonium. 相似文献
998.
999.