全文获取类型
收费全文 | 920篇 |
免费 | 79篇 |
国内免费 | 2篇 |
出版年
2023年 | 3篇 |
2022年 | 16篇 |
2021年 | 31篇 |
2020年 | 16篇 |
2019年 | 15篇 |
2018年 | 30篇 |
2017年 | 39篇 |
2016年 | 36篇 |
2015年 | 58篇 |
2014年 | 55篇 |
2013年 | 63篇 |
2012年 | 78篇 |
2011年 | 80篇 |
2010年 | 54篇 |
2009年 | 39篇 |
2008年 | 61篇 |
2007年 | 49篇 |
2006年 | 40篇 |
2005年 | 30篇 |
2004年 | 22篇 |
2003年 | 25篇 |
2002年 | 18篇 |
2001年 | 15篇 |
2000年 | 18篇 |
1999年 | 12篇 |
1998年 | 8篇 |
1997年 | 6篇 |
1996年 | 11篇 |
1995年 | 9篇 |
1994年 | 2篇 |
1993年 | 5篇 |
1992年 | 6篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1988年 | 5篇 |
1987年 | 3篇 |
1986年 | 2篇 |
1985年 | 4篇 |
1984年 | 5篇 |
1982年 | 2篇 |
1981年 | 2篇 |
1979年 | 2篇 |
1977年 | 4篇 |
1976年 | 2篇 |
1971年 | 1篇 |
1969年 | 1篇 |
1968年 | 2篇 |
1966年 | 1篇 |
1965年 | 2篇 |
1964年 | 1篇 |
排序方式: 共有1001条查询结果,搜索用时 31 毫秒
931.
Larissa Tavares Cyrino Alexandra Paiva Araújo Paulo Pinto Joazeiro Cristina Pontes Vicente Selma Giorgio 《Tissue & cell》2012,44(6):401-408
Autophagy is the primary mechanism of degradation of cellular proteins and at least two functions can be attributed to this biological phenomenon: increased nutrient supply via recycling of the products of autophagy under nutrient starvation; and antimicrobial response involved in the innate immune system. Many microorganisms induce host cell autophagy and it has been proposed as a pathway by which parasites compete with the host cell for limited resources. In this report we provide evidence that the intracellular parasite Leishmania amazonensis induces autophagy in macrophages. Using western blotting, the LC3II protein, a marker of autophagosomes, was detected in cell cultures with a high infection index. Macrophages infected with L. amazonensis were examined by transmission electronic microscopy, which revealed enlarged myelin-like structures typical late autophagosome and autolysosome. Other evidence indicating autophagy was Lysotracker red dye uptake by the macrophages. Autophagy also occurs in the leishmaniasis skin lesions of BALB/c mice, detected by immunohistochemistry with anti-LC3II antibody. In this study, autophagy inhibitor 3-methyladenine (3MA) reduced the infection index, while autophagy inductors, such as rapamycin or starvation, did not alter the infection index in cultivated macrophages, suggesting that one aspect of the role of autophagy could be the provision of nutritive support to the parasite. 相似文献
932.
M. MAROLI L. JALOUK M. AL AHMED R. BIANCHI G. BONGIORNO C. KHOURY L. GRADONI 《Medical and veterinary entomology》2009,23(2):148-154
Aspects of the bionomics of phlebotomine sandflies (Diptera: Psychodidae) were studied from June to November 2005 in three foci of anthroponotic cutaneous leishmaniasis (ACL) in Aleppo Governorate, Syria, where the agent Leishmania tropica (Wright) (Kinetoplastida: Trypanosomatidae) is transmitted by Phlebotomus sergenti Parrot. Syria has been designated by the World Health Organization as one of four countries in the Old World where cutaneous leishmaniasis is hyperendemic, but little is known about the biology of local vector populations. Standard collections by sticky traps showed two peaks in density, in June and late August. In total, 1840 sandflies were caught, comprising five species: Phlebotomus papatasi (Scopoli) (68.0%); P. sergenti (25.4%); Sergentomyia minuta (Rondani) (6.4%); Phlebotomus tobbi Adler & Theodor (0.1%), and Phlebotomus mascittii canaaniticus Adler & Theodor (0.1%). Similar numbers of P. sergenti were caught indoors (246 specimens) and outdoors (222), whereas P. papatasi was significantly more abundant indoors (1096 specimens) than outdoors (156) (χ2 = 241, P < 0.01). In total, 212 blood-fed females were tested for host blood determination, of which 176 (83.0%) reacted with anti-species reagent. Results from 20 P. sergenti suggest that this species is an opportunistic feeder, imbibing human, ovine, avian, bovine and feline blood, although more bloodmeals were taken from humans and cattle than expected in relation to the relative proportions of potential hosts present (the forage ratio, FR). The bionomics of P. sergenti are discussed in relation to the inefficacy of control campaigns based on indoor spraying with residual insecticides that have been implemented by the Syrian Ministry of Health to control the epidemics of ACL in the Aleppo Governorate. 相似文献
933.
Augusta Silva Andreia S. P. Sousa Cláudia Costa Silva Rubim Santos João Manuel R. S. Tavares Filipa Sousa 《Somatosensory & motor research》2017,34(3):185-188
Most stroke lesions occur in the middle cerebral artery territory, presenting a high probability of damage of pathways with predominant ipsilesional disposition, mainly related to postural control. Despite the high probability of bilateral postural control dysfunction based on neuroanatomical fundaments, both research and clinical rehabilitation involving stroke subjects have been focused on contralesional side (also named affected side) impairments, while ipsilesional side (also named non-affected side) impairments have been attributed to an adaptive strategy. This paper aims to present a critical understanding about the state-of-the-art that sustains the hypothesis that stroke subjects with middle cerebral artery territory lesion at the subcortical level show an atypical behaviour in the ipsilateral side associated with the lesion itself and the possible implications. 相似文献
934.
935.
Bizuti Matheus Ribeiro Starck Édina da Silva Fagundes Kimberly Kamila Puhle Josiano Guilherme Lima Lucas Medeiros de Oliveira Natan Rodrigues de Sousa Silva Guilherme Vinicio Silva Débora Tavares Resende e 《Molecular and cellular biochemistry》2022,477(6):1725-1737
Molecular and Cellular Biochemistry - Respiratory infections of viral origin have become the leading cause of infectious diseases in the world. In 2020, the World Health Organization (WHO) declared... 相似文献
936.
Alejandro O Luquetti Suelene Brito do Nascimento Tavares Liliane da Rocha Siriano Rozangela Amaral de Oliveira Dayse Elizabeth Campos Cicilio Alves de Morais Enio Chaves de Oliveira 《Memórias do Instituto Oswaldo Cruz》2015,110(3):369-376
Transmission of Trypanosoma cruzi during pregnancy is estimated to
occur in less than 20% of infected mothers; however, the etiopathogenesis is not
completely understood. The Centre for Studies on Chagas Disease provides confirmation
of T. cruzi infection for individuals living in central Brazil.
In this retrospective hospital-based study, all requests for diagnosis of T.
cruzi infection in individuals less than 21 years old from 1994-2014 were
searched. We end with 1,211 individuals and their respective infected mothers.
Congenital transmission of infection was confirmed in 24 individuals (2%) in central
Brazil, an area where the main T. cruzi lineage circulating in
humans is TcII. This low prevalence of congenital Chagas disease is discussed in
relation to recent findings in the south region of Brazil, where TcV is the main
lineage and congenital transmission has a higher prevalence (approximately 5%),
similar to frequencies reported in Argentina, Paraguay and Bolivia. This is the first
report to show geographical differences in the rates of congenital transmission
of T. cruzi and the relationship between the prevalence of
congenital transmission and the type of Tc prevalent in each region. 相似文献
937.
938.
Some studies require fresh biological material for their development. Ant colonies have been reared under laboratory conditions
for scientific purposes, and several methodologies for leafcutter ants have been reported in the literature. However, these
methods are not well adapted for rearing basal Attini. In this study, we proposed a methodology for rearing basal Attini species
in the laboratory based on the evaluation of colonies of the genus Mycetophylax. The complete system consists of two round translucent polypropylene containers inserted one inside the other, where one
serves as a chamber proper and the other as a foraging area. Both containers are sealed with their lids, protecting the environment
against desiccation. From a total of 29 colonies collected in the field, 22 colonies survived for at least 30 weeks, and Mycetophylax morschi was the most adapted for rearing under laboratory conditions. The main problem with rearing basal Attini in the laboratory
is the loss of moisture. Thus, the method applied here may be adopted for rearing other basal Attini, as well as other ant
species very sensitive to moisture variation. 相似文献
939.
Bobrowiec Paulo Estefano D. Farneda Fábio Z. Nobre Carla Clarissa da Cunha Tavares Valéria 《Biodiversity and Conservation》2022,31(4):1359-1377
Biodiversity and Conservation - Hydroelectric dams are among the main cause of anthropogenic impacts in tropical environments. Damming interrupts the continuity of the river and produces the... 相似文献
940.