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121.
Kenta Masuda Tatsuyuki Chiyoda Naoyuki Sugiyama Aldo Segura-Cabrera Yasuaki Kabe Arisa Ueki Koji Banno Makoto Suematsu Daisuke Aoki Yasushi Ishihama Hideyuki Saya Shinji Kuninaka 《PloS one》2015,10(2)
In budding yeast, the Mitotic Exit Network (MEN) regulates anaphase promoting complex/cyclosome (APC/C) via the Dbf2-Cdc14 signaling cascade. Dbf2 kinase phosphorylates and activates Cdc14 phosphatase, which removes the inhibitory phosphorylation of the APC/C cofactor Cdh1. Although each component of the MEN was highly conserved during evolution, there is presently no evidence supporting direct phosphorylation of CDC14 by large tumor suppressor kinase 1 (LATS1), the human counterpart of Dbf2; hence, it is unclear how LATS1 regulates APC/C. Here, we demonstrate that LATS1 phosphorylates the Thr7 (T7) residue of the APC/C component CDC26 directly. Nocodazole-induced phosphorylation of T7 was reduced by knockdown of LATS1 and LATS2 in HeLa cells, indicating that both of these kinases contribute to the phosphorylation of CDC26 in vivo. The T7 residue of CDC26 is critical for its interaction with APC6, a tetratricopeptide repeat-containing subunit of APC/C, and mutation of this residue to Asp (T7D) reduced the interaction of CDC26 with APC6. Replacement of endogenous CDC26 in HeLa cells with exogenous phosphor-mimic T7D-mutated CDC26 increased the elution size of APC/C subunits in a gel filtration assay, implying a change in the APC/C assembly upon phosphorylation of CDC26. Furthermore, T7D-mutated CDC26 promoted the ubiquitination of polo-like kinase 1, a well-known substrate of APC/C. Overall, these results suggest that LATS1/2 are novel kinases involved in APC/C phosphorylation and indicate a direct regulatory link between LATS1/2 and APC/C. 相似文献
122.
Shogo Higaki Tomomi Nishie Takaaki Todo Reiko Teshima Kenichiro Kusumi Risa Mitsumori Ikuo Tooyama Yasuhiro Fujioka Toshihiro Kawasaki Noriyoshi Sakai Tatsuyuki Takada 《Journal of fish biology》2021,99(4):1341-1347
Fishes expressing a fluorescent protein in germ cells are useful to perform germ cell transfer experiments for conservation study. Nonetheless, no such fish has been generated in endangered endemic fishes. In this study, we tried to produce a fish expressing Venus fluorescent protein in germ cells using Honmoroko (Gnathopogon caerulescens), which is one of the threatened small cyprinid endemic to the ancient Lake Biwa in Japan. To achieve germ cell-specific expression of Venus, we used piwil1 (formally known as ziwi) promoter and Tol2 transposon system. Following the co-injection of the piwil1-Venus expression vector and the Tol2 transposase mRNA into fertilized eggs, presumptive transgenic fish were reared. At 7 months of post-fertilization, about 19% (10/52) of the examined larvae showed Venus fluorescence in their gonad specifically. Immunohistological staining and in vitro spermatogenesis using gonads of the juvenile founder fish revealed that Venus expression was detected in spermatogonia and spermatocyte in male, and oogonia and stage I and II oocytes in female. These results indicate that the Tol2 transposon and zebrafish piwil1 promoter enabled gene transfer and germ cell-specific expression of Venus in G. caerulescens. In addition, in vitro culture of juvenile spermatogonia enables the rapid validation of temporal expression of transgene during spermatogenesis. 相似文献
123.
Takehisa?Matsumoto Masatomo?Kawakubo Mayumi?Shiohara Toshiko?Kumagai Eiko?Hidaka Kazuyoshi?Yamauchi Kozue?Oana Kenji?Matsuzawa Hiroyoshi?Ota Yoshiyuki?KawakamiEmail author 《Journal of microbiology (Seoul, Korea)》2009,47(2):201-207
“Helicobacter heilmannii” is an uncultivable spiral-shaped bacterium inhabiting the human gastric mucosa. It is larger and more tightly-coiled than
H. pylori. We encountered a patient with chronic gastritis infected a “H. heilmannii”-like organism (HHLO), designated as SH6. Gastric mucosa derived from the patient was orally ingested by specific pathogen
free mice. Colonization of the mice by SH6 was confirmed by electron microscopy of gastric tissue specimens. In an attempt
to characterize SH6, 16S rRNA and urease genes were sequenced. The 16S rRNA gene sequence was most similar (99.4%; 1,437/1,445
bp) to HHLO C4E from a cheetah. However, the urease gene sequence displayed low similarity (81.7%; 1,240/1,516 bp) with HHLO
C4E. Taxonomic analysis disclosed that SH6 represents a novel strain and should constitute a novel taxon in the phylogenetic
trees, being discriminated from any other taxon, with the ability of infecting human gastric mucosa. 相似文献
124.
125.
A novel method, based on the hemolytic screening of a cDNA phage library, was developed to isolate cDNAs encoding grammistins (antibacterial peptide toxins) of the soapfish Pogonoperca punctata. As a result, cDNAs encoding six grammistins were isolated and elucidated for their nucleotide sequences. In common with the grammistins, the precursor protein is composed of a highly conserved signal peptide, a considerably conserved propeptide that is characterized to contain a pair of basic residues (Lys-Arg) at plural positions including the C-terminus and one copy of a mature peptide. This precursor organization is similar to those of dermaseptins, antibacterial peptides from the frog skin. 相似文献
126.
The lesser Japanese mole, Mogera imaizumii, recognized by Motokawa and Abe (1996), occurs in eastern Honshu, western Honshu and Shikoku. Mitochondrial and nuclear DNA were analyzed for mole samples from eastern Honshu to elucidate intraspecific differentiation. Analyses of sequences of the mitochondrial cytochrome b gene (Cytb) and of a restriction fragment length polymorphism of the nuclear 28S ribosomal RNA gene spacer (rDNA-RFLP) revealed two genetic types, partially corresponding to Hutterer's (1993) taxa, M. wogura (= M. imaizumii) and M. minor. Most samples showed either of two combinations of mitochondrial / nuclear gene types. However, two specimens showed a different combination. This incongruent combination of mitochondrial and nuclear genes might have derived, in part, from an introgression event between genetically differentiated populations after secondary contact during the evolutionary history of the lesser Japanese mole in eastern Honshu. 相似文献
127.
Kuwayama K Inoue H Kanamori T Tsujikawa K Miyaguchi H Iwata YT Miyauchi S Kamo N 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2008,867(1):78-83
The aim of this work was to develop and validate a method for analysing amphetamine-type stimulants (ATSs) and their metabolites in plasma, urine and bile by liquid chromatography with a strong cation-exchange column-tandem mass spectrometry, and to apply it to the pharmacokinetic study of ATSs. 3,4-Methylenedioxymethamphetamine, methamphetamine, ketamine and their main metabolites, 4-hydroxy-3-methoxymethamphetamine, 3,4-methylenedioxyamphetamine, p-hydroxymethamphetamine, amphetamine and norketamine, were simultaneously quantified by the new method (50-5000 ng/ml). The coefficients of variation and the percent deviations for the eight compounds were in the range of 0.2 to 5.3% and -9.4 to +12.8%, respectively. The recoveries were over 90% in all biological samples tested. This method was effective for the separation and the identification of ATSs and their main metabolites having amine moieties in plasma, urine and bile, and was applicable to pharmacokinetic analysis of methamphetamine, ketamine and their main metabolites in biological samples. This analytical method should be useful for the pharmacokinetic analysis of ATSs. 相似文献
128.
Monkey embryonic stem (ES) cells share similar characteristics to human ES cells and provide a primate model of allotransplantation, which allows to validate efficacy and safety of cell transplantation therapy in regenerative medicine. Bone morphogenetic protein 4 (BMP4) is known to promote trophoblast differentiation in human ES cells in contrast to mouse ES cells where BMP4 synergistically maintains self-renewal with leukemia inhibitory factor (LIF), which represents a significant difference in signal transduction of self-renewal and differentiation between murine and human ES cells. As the similarity of the differentiation mechanism between monkey and human ES cells is of critical importance for their use as a primate model system, we investigated whether BMP4 induces trophoblast differentiation in monkey ES cells. Interestingly, BMP4 did not induce trophoblast differentiation, but instead induced primitive endoderm differentiation. Prominent downregulation of Sox2, which plays a pivotal role not only in pluripotency but also placenta development, was observed in cells treated with BMP4. In addition, upregulation of Hand1, Cdx2, and chorionic gonadotropin beta (CG-beta), which are markers of trophoblast, was not observed. In contrast, BMP4 induced significant upregulation of Gata6, Gata4, and LamininB1, suggesting differentiation into the primitive endoderm, visceral endoderm, and parietal endoderm, respectively. The threshold of BMP4 activity was estimated as about 10 ng/mL. These findings suggest that BMP4 induced differentiation into the primitive endoderm lineage but not into trophoblast in monkey ES cells. 相似文献
129.
Cathepsin E is an endolysosomal aspartic proteinase predominantly expressed in cells of the immune system and has been implicated in various physiological and pathological processes. Because of physiological substrates of cathepsin E have not yet been identified, however, the physiological significance of this protein still remains speculative. To better understand the physiological significance of cathepsin E in the mammary gland, we investigated the effect of the deficiency of this protein on the gene expression profile of the tissue. Here we used mammary glands derived from multiparous and non-pregnant 11-month-old syngenic wild-type (CatE(+/+)) and cathepsin E-deficient (CatE(-/-)) mice for extraction of total RNA from each tissue and subsequent mRNA amplification, DNA fragmentation, and hybridization with cDNA mixroarray chips. A total of 654 genes were identified as overexpressed (>2-fold) in CatE(-/-) mammary glands compared with CatE(+/+) counterparts. These included genes related to signal transduction, immune responses, growth factor activity, and milk proteins, which occupied a large portion of the gene fragments identified as overexpressed. In contrast, a total of 665 known genes were identified as underexpressed in the mammary gland of CatE(-/-) mice compared with CatE(+/+) counterparts. These included genes related to cytoskeleton, cell differentiation, cell cycle arrest and apoptosis, which occupied the majority of the gene fragments identified as underexpressed. The results thus suggest that cathepsin E in mammary glands plays a crucial role in the regulation of proteins involved in signaling, development, differentiation and proliferation in the mammary gland. 相似文献
130.
Yang H Kawakubo K Wong H Ohning G Walsh J Taché Y 《American journal of physiology. Gastrointestinal and liver physiology》2000,279(3):G575-G581
The site of action of peripheral peptide YY (PYY)-induced inhibition of vagally stimulated gastric acid secretion was studied using immunoneutralization with PYY antibody in urethan-anesthetized rats. Gastric acid secretion (59+/-7 micromol/90 min) stimulated by intracisternal injection of the stable thyrotropin-releasing hormone (TRH) analog RX-77368 (14 pmol/rat) was dose-dependently inhibited by 52%, 69%, and 83% by intravenous infusion of 0.25, 0.5, and 1.0 nmol. kg(-1) x h(-1) PYY, respectively. PYY or PYY(3-36) (2.4 pmol/rat) injected intracisternally also inhibited the acid response to intracisternal RX-77368 by 73% and 80%, respectively. Intravenous pretreatment with PYY antibody (4.5 mg/rat), which shows a 35% cross-reaction with PYY(3-36) by RIA, completely prevented the inhibitory effect of intravenously infused PYY (1 nmol x kg(-1) x h(-1)). When injected intracisternally, the PYY antibody (280 microg/rat) reversed intracisternal PYY (2.4 pmol)- and intravenous PYY (1 nmol x kg(-1) x h(-1))-induced inhibition of acid response to intracisternal RX-77368 by 64% and 93.5%, respectively. These results provide supporting evidence that peripheral PYY inhibits central vagal stimulation of gastric acid secretion through an action in the brain. 相似文献