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11.
Thompson JE Kutateladze TG Schuster MC Venegas FD Messmore JM Raines RT 《Bioorganic chemistry》1995,23(4):471-481
Bovine pancreatic ribonuclease A (RNase A) catalyzes the cleavage of the P-O(5') bond in RNA. Although this enzyme has been the object of much landmark work in bioorganic chemistry, the nature of its rate-limiting transition state and its catalytic rate enhancement had been unknown. Here, the value of k(cat)/K(m) for the cleavage of UpA by wild-type RNase A was found to be inversely related to the concentration of added glycerol. In contrast, the values of k(cat)/K(m) for the cleavage of UpA by a sluggish mutant of RNase A and the cleavage of the poor substrate UpOC(6)H(4)-p-NO(2) by wild-type RNase A were found to be independent of glycerol concentration. Yet, UpA cleavage by the wild-type and mutant enzymes was found to have the same dependence on sucrose concentration, indicating that catalysis of UpA cleavage by RNase A is limited by desolvation. The rate of UpA cleavage by RNase A is maximal at pH 6.0, where k(cat) = 1.4 × 10(3) s(-1) and k(cat)/K(m) = 2.3 × 10(6) M(-1)s(-1) at 25°C. At pH 6.0 and 25°C, the uncatalyzed rate of [5,6-(3)H]Up[3,5,8-(3)H]A cleavage was found to be k(uncat) = 5 × 10(-9) s(-1) (t(1/2) = 4 years). Thus, RNase A enhances the rate of UpA cleavage by 3 × 10(11)-fold by binding to the transition state for P-O(5') bond cleavage with a dissociation constant of <2 × 10(-15) M. 相似文献
12.
Hummel Herman Bogaards Roelof Bek Tatiana Polishchuk Lennard Amiard-Triquet Claude Bachelet Guy Desprez Michel Strelkov Peter Sukhotin Alex Naumov Andrei Dahle Salve Denisenko Stanislav Gantsevich Michael Sokolov Kirill Wolf Lein de 《Hydrobiologia》1997,355(1-3):127-138
The stress sensitivity, determined in copper exposureexperiments and in survival in air tests, and thegenetic structure, measured by means of isoenzymeelectrophoresis, were assessed in populations of theBaltic clam Macoma balthica (L.) from itssouthern to its northern distribution limit, in orderto test the hypotheses that near the distributionlimit the clams would be more stress sensitive andwould have a lower genetic variability. Thepopulations in west and north Europe show a stronggenetic resemblance. The populations in the sub-ArcticWhite Sea are genetically slightly different, and showa low stress sensitivity. The populations in theArctic Pechora Sea are genetically very distant fromthe other populations, and show the lowest stresssensitivity. Near the southern distribution limit, inagreement with the hypotheses, genetic variability islow and stress sensitivity high. On the other hand, incontrast to expectation, near the northerndistribution limit, in the populations of the PechoraSea, the genetic variability was higher, thus notreduced, and the stress sensitivity was low comparedto all other populations. Yet, it remains a questionif such is due to gradual physiologicalacclimatization (and ongoing differential selection)or to genetic adaptation. 相似文献
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Tatiana Kozhina Lubomira Stateva Pencho Venkov 《Molecular & general genetics : MGG》1979,170(3):351-354
Summary The genetic analysis of VY1160 sorbitol dependent, osmotic sensitive yeast mutant led to the identification of three different nuclear recessive mutations. Two of them, designated sorb- and ts1 are closely linked to one another. The mutation sorb- determines the lysis, while the mutation tsl increases the ability for lysis of the sorbitol dependent cells. The third mutation ts2 segregates independently from the other two and confers the sensitivity of VY1160 mutant cells towards rifampicin. 相似文献
15.
Myeloproliferative virus, a cloned murine sarcoma virus with spleen focus-forming properties in adult mice. 总被引:29,自引:16,他引:13 下载免费PDF全文
W Ostertag K Vehmeyer B Fagg I B Pragnell W Paetz M C Le Bousse F Smadja-Joffe B Klein C Jasmin H Eisen 《Journal of virology》1980,33(2):573-582
Myeloproliferative virus, derived from Moloney sarcoma virus, causes erythroleukemia and myeloid leukemia in adult mice. This virus is also capable of fibroblast transformation in vitro. The virus consists of two separable biological entities which have been cloned. The helper virus component caused no visible changes in adult mice, whereas the defective virus induced both spleen focus formation and a large increase in erythroid precursor cells but retained the sarcoma virus property of transforming fibroblasts in vitro. Thus, myeloproliferative virus is the first murine sarcoma virus which induces erythroleukemia in adult animals. 相似文献
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The Middle Longcraig Limestone (late Visean) at Catcraig, Scotland, is densely covered with large (about 1 m in diameter) hollows, which are surrounded by numerous fossilized roots. The latter represent the positions of tree-sized plants belonging to a fossil forest. This paper aims to reconstruct its ecology based on paleosol properties, size and spatial distribution of root-casts, rooting system morphology and characteristics of coalified plant remnants. The obtained data are consistent with the supposition that the Visean peat wetland forest of Catcraig represents a Cordaitalean dominated community composed of mono-sized, most probably, even-aged giant trees. The peaty paleosol (Histosol) contains pyrite and siderite, indicating reducing and acidic conditions, whilst the abundant presence of gypsum testifies periods of elevated salinity. The adaptation of trees to such conditions is supported by morphologically evident large root mass (typically more than 1000 individual roots per cast). Good preservation of plant tissues in peat, in addition to other paleosol and rooting system characteristics allow us to conclude that the trees occupied seacoast settings or lagoons, which were not permanently flooded. 相似文献
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Loss of function of Arabidopsis NADP‐malic enzyme 1 results in enhanced tolerance to aluminum stress
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Fanny E. Hartmann Alodie Snirc Amandine Cornille Ccile God Pascal Touzet Fabienne Van Rossum Elisabeth Fournier Stphanie Le Prieur Jacqui Shykoff Tatiana Giraud 《Molecular ecology》2020,29(6):1154-1172
Study of the congruence of population genetic structure between hosts and pathogens gives important insights into their shared phylogeographical and coevolutionary histories. We studied the population genetic structure of castrating anther‐smut fungi (genus Microbotryum) and of their host plants, the Silene nutans species complex, and the morphologically and genetically closely related Silene italica, which can be found in sympatry. Phylogeographical population genetic structure related to persistence in separate glacial refugia has been recently revealed in the S. nutans plant species complex across Western Europe, identifying several distinct lineages. We genotyped 171 associated plant–pathogen pairs of anther‐smut fungi and their host plant individuals using microsatellite markers and plant chloroplastic single nucleotide polymorphisms. We found clear differentiation between fungal populations parasitizing S. nutans and S. italica plants. The population genetic structure of fungal strains parasitizing the S. nutans plant species complex mirrored the host plant genetic structure, suggesting that the pathogen was isolated in glacial refugia together with its host and/or that it has specialized on the plant genetic lineages. Using random forest approximate Bayesian computation (ABC‐RF), we found that the divergence history of the fungal lineages on S. nutans was congruent with that previously inferred for the host plant and probably occurred with ancient but no recent gene flow. Genome sequences confirmed the genetic structure and the absence of recent gene flow between fungal genetic lineages. Our analyses of individual host–pathogen pairs contribute to a better understanding of co‐evolutionary histories between hosts and pathogens in natural ecosystems, in which such studies remain scarce. 相似文献