全文获取类型
收费全文 | 1217篇 |
免费 | 80篇 |
出版年
2021年 | 7篇 |
2018年 | 8篇 |
2017年 | 9篇 |
2016年 | 10篇 |
2015年 | 39篇 |
2014年 | 19篇 |
2013年 | 160篇 |
2012年 | 43篇 |
2011年 | 33篇 |
2010年 | 38篇 |
2009年 | 34篇 |
2008年 | 36篇 |
2007年 | 45篇 |
2006年 | 44篇 |
2005年 | 37篇 |
2004年 | 30篇 |
2003年 | 37篇 |
2002年 | 29篇 |
2001年 | 29篇 |
2000年 | 52篇 |
1999年 | 31篇 |
1998年 | 13篇 |
1997年 | 9篇 |
1996年 | 11篇 |
1995年 | 15篇 |
1992年 | 30篇 |
1991年 | 33篇 |
1990年 | 37篇 |
1989年 | 29篇 |
1988年 | 38篇 |
1987年 | 33篇 |
1986年 | 25篇 |
1985年 | 33篇 |
1984年 | 9篇 |
1983年 | 16篇 |
1982年 | 13篇 |
1981年 | 6篇 |
1980年 | 6篇 |
1979年 | 15篇 |
1978年 | 12篇 |
1977年 | 13篇 |
1976年 | 11篇 |
1975年 | 10篇 |
1974年 | 10篇 |
1973年 | 14篇 |
1972年 | 13篇 |
1971年 | 10篇 |
1970年 | 13篇 |
1967年 | 12篇 |
1966年 | 5篇 |
排序方式: 共有1297条查询结果,搜索用时 15 毫秒
71.
72.
73.
74.
Kazuyuki Nakamura Hirofumi Kodera Tenpei Akita Masaaki Shiina Mitsuhiro Kato Hideki Hoshino Hiroshi Terashima Hitoshi Osaka Shinichi Nakamura Jun Tohyama Tatsuro Kumada Tomonori Furukawa Satomi Iwata Takashi Shiihara Masaya Kubota Satoko Miyatake Eriko Koshimizu Kiyomi Nishiyama Mitsuko Nakashima Yoshinori Tsurusaki Noriko Miyake Kiyoshi Hayasaka Kazuhiro Ogata Atsuo Fukuda Naomichi Matsumoto Hirotomo Saitsu 《American journal of human genetics》2013
75.
Yoshikazu Izumi Kuninori Sato Yoshiki Tani Koichi Ogata 《Bioscience, biotechnology, and biochemistry》2013,77(11):2683-2684
Vitamin B6 is synthesized by green Cytisus scoparius callus and green Phellodendron amurense callus cultured on Linsmaier and Skoog Agar-medium with 10?5m of ±-naphthaleneacetic acid (NAA) and 10?6 m of 6-benzyladenine (BA). Even when thiamine and inositol were omitted from this medium, the growth and vitamin B6 content of Cytisus scoparius callus did not change. Vitamin B6 contents of clones of the calluses varied and were unstable during long-term subculture. Clonal selection was repeated to obtain stable strains with high vitamin B6 content, and the vitamin B6 content of one strain of green Cytisus scoparius callus became 4-times higher than that of the green leaves. 相似文献
76.
Hideaki Yamada Osao Adachi Koichi Ogata 《Bioscience, biotechnology, and biochemistry》2013,77(2):117-123
The amine oxidase was found to be formed in mycelia of fungi when they were grown on monoamines or diamines as sole nitrogen sources. The maximal formation of enzyme was observed in the initial stage of growth, then the enzyme disappeared semilogarithmically. Other sources of nitrogen, such as ammonia, nitrate, urea and amino acids, were fully inactive for the enzyme formation. Furthermore, ammonia repressed the enzyme formation by fungi. The amine oxidase of fungi resembled in substrate specificity the monoamine oxidase of animal tissues. The enzyme oxidized preferentially aliphatic monoamines of C3–C6. Agmatine and histamine were also oxidized but in lower rates. Benzylamine was well oxidized by the enzymes of Aspergillus niger and Penicillium chrysogenum, but not by the enzymes of Monascus anka and Fusarium bulbigenum. Polyamines were not oxidized by the fungal enzymes. 相似文献
77.
Takuo Sakai Tatsurokuro Tochikura Koichi Ogata 《Bioscience, biotechnology, and biochemistry》2013,77(8):737-741
In this study, the authors developed a simplified method for the separation and the quantitative determination of nucleosides and bases, using paper electraophoretic technique. By this method, nucleosides and bases were well separated and determined in a fairly short time. Thus, this method was expected to be as accurate as the published methods and was believed to be a better method for both quantitative determination and detection of the nucleosides and bases in a large number of samples. 相似文献
78.
79.
Kichitaro Kawaguchi Koichi Ogata Tatsurokuro Tochikura 《Bioscience, biotechnology, and biochemistry》2013,77(6):908-918
Effects of various factors including incubation time, water content of airdried cells, concentration and pH of KH2PO4–K2HPO4 mixture, d-glucose concentration, MgSO4 concentration, GMP concentration, cell concentration, aeration and various kinds of carbohydrates on the fermentative production of GDP-mannose, GDP and GTP from 5′-GMP by air-dried cells of baker’s yeast were investigated. The water content of air-dried cells was the most important factor in the fermentation. When the air-dried cells of baker’s yeast (100 mg/ml) were incubated with 5′-GMP (20 μmoles/ml), d-glucose (800 μmoles/ml), potassium phosphate buffer (360 μmoles/ml, pH 7.0), and MgSO4 (20 μmoles/ml), 2-hr incubation gave GDP in 20% yield and GTP in 61.1% yield, GDP-mannose being produced in 45% yield after 8-hr incubation. The phosphorylation of 5′-AMP, 5′-dAMP, 5′-dGMP 5′-CMP and 5′-UMP was also observed in high yields under the same conditions. 相似文献
80.
Fusako Kawai Kensei Maezato Hideaki Yamada Koichi Ogata 《Bioscience, biotechnology, and biochemistry》2013,77(9):1675-1679
The formation of D-pantothenic acid-α-glucoside (PaA-α-G) was found from D-pantothenic acid (PaA) and maltose in incubation mixtures of microorganisms, especially Saccharomyces yeasts and Sporobolomyces coralliformis IFO 1032. The reaction conditions were investigated for formation of PaA-α-G by resting cells of Spor. coralliformis. The formation of the compound increased with PaA concentration (3~20 mg/ml). The yield was maximum at 5~10 mg/ml of PaA. Cetyl trimethyl ammonium bromide (0.1 %) promoted the formation of PaA-α-G. Sucrose was the optimal α-glucosyl donor. When 30 mg/ml of sucrose was fed to the reaction mixture (initial sucrose, 100 mg/ml; and PaA, 10 mg/ml) at 12-hr intervals, 5.74 mg/ml (3.30 mg/ml as PaA) of PaA-α-G was formed in 48-hr incubation at 28°C with shaking. PaA-α-G was also formed by yeast α-glucosidase, mold maltase and the cell-free extract of Spor. coralliformis. The compound showed approximately 9~10% and 0.1~0.3% (molar ratio) of activity of PaA for Saccharomyces carlsbergensis ATCC 9080 and Lactobacillus plantarum ATCC 8014, respectively. The compound had the same microbiological activity as authentic 4′-O-(α-D-glucopyranosyl)-D-pantothenic acid. 相似文献