全文获取类型
收费全文 | 234篇 |
免费 | 16篇 |
出版年
2022年 | 2篇 |
2021年 | 2篇 |
2020年 | 2篇 |
2019年 | 3篇 |
2018年 | 4篇 |
2017年 | 6篇 |
2016年 | 9篇 |
2015年 | 13篇 |
2014年 | 15篇 |
2013年 | 14篇 |
2012年 | 10篇 |
2011年 | 20篇 |
2010年 | 7篇 |
2009年 | 11篇 |
2008年 | 17篇 |
2007年 | 12篇 |
2006年 | 16篇 |
2005年 | 13篇 |
2004年 | 20篇 |
2003年 | 10篇 |
2002年 | 8篇 |
2001年 | 8篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 4篇 |
1997年 | 2篇 |
1996年 | 4篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1982年 | 1篇 |
排序方式: 共有250条查询结果,搜索用时 281 毫秒
91.
Pikas (Ochotona princeps: Lagomorpha) build caches of vegetation (“haypiles”), which serve as a food source during winter in alpine and subalpine
habitats. Haypiles appear to degrade over time and form patches of nutrient-rich soils in barren talus and scree areas. We
sampled soils underneath and next to haypiles, and plants growing on and near haypiles in an alpine cirque in northwestern
Wyoming, USA, to determine the effects of pika food caches on N, C, and C/N ratios in soils and plants. We found that (1)
haypile soils had significantly higher carbon and nitrogen levels and lower C/N ratios than both adjacent soils and soils
in the general study area, (2) two of three plant species tested (Polemonium viscosum and Oxyria digyna) had significantly higher levels of tissue percent N when growing on haypile soils, and (3) total standing plant biomass
at the study site increased with soil percent N suggesting that vegetation was nitrogen limited. Pikas may therefore function
as allogenic ecosystem engineers by modulating nutrient availability to plants.
Received: 5 July 1997 / Accepted: 30 November 1997 相似文献
92.
Shinji Imai Marko Kaksonen Erkki Raulo Tarja Kinnunen Carole Fages Xiaojuan Meng Merja Lakso Heikki Rauvala 《The Journal of cell biology》1998,143(4):1113-1128
Bone has an enormous capacity for growth, regeneration, and remodeling. This capacity is largely due to induction of osteoblasts that are recruited to the site of bone formation. The recruitment of osteoblasts has not been fully elucidated, though the immediate environment of the cells is likely to play a role via cell– matrix interactions. We show here that heparin-binding growth-associated molecule (HB-GAM), an extracellular matrix–associated protein that enhances migratory responses in neurons, is prominently expressed in the cell matrices that act as target substrates for bone formation. Intriguingly, N-syndecan, which acts as a receptor for HB-GAM, is expressed by osteoblasts/osteoblast precursors, whose ultrastructural phenotypes suggest active cell motility. The hypothesis that HB-GAM/N-syndecan interaction mediates osteoblast recruitment, as inferred from developmental studies, was tested using osteoblast-type cells that express N-syndecan abundantly. These cells migrate rapidly to HB-GAM in a haptotactic transfilter assay and in a migration assay where HB-GAM patterns were created on culture wells. The mechanism of migration is similar to that previously described for the HB-GAM–induced migratory response of neurons. Our hypothesis that HB-GAM/N-syndecan interaction participates in regulation of osteoblast recruitment was tested using two different in vivo models: an adjuvant-induced arthritic model and a transgenic model. In the adjuvant-induced injury model, the expression of HB-GAM and of N-syndecan is strongly upregulated in the periosteum accompanying the regenerative response of bone. In the transgenic model, the HB-GAM expression is maintained in mesenchymal tissues with the highest expression in the periosteum. The HB-GAM transgenic mice develop a phenotype characterized by an increased bone thickness. HB-GAM may thus play an important role in bone formation, probably by mediating recruitment and attachment of osteoblasts/osteoblast precursors to the appropriate substrates for deposition of new bone. 相似文献
93.
Summary The model of exploitation ecosystems was re-analysed, assuming that habitat patches are so small that they form only parts of the home range of an individual predator. For habitat complexes where productive patches abound, the results suggested that predation will strongly spill over from productive patches, which set the tune for population dynamics within the whole landscape, to barren ones. This result conforms to the one obtained by T. Oksanen by assuming despotic habitat choice and essentially larger patch sizes. For habitat complexes heavily dominated by the barren habitat, spillover predation was predicted to be weak, as was the case in her large patch model. Unlike in her analysis, however, predation pressure was substantially reduced also within the productive habitat. In habitat complexes where patches are so small that they are exploited in a fine-grained manner, predation pressure was always found to be more intense in the barren habitat, contrary to the predictions of the original model of exploitation ecosystems. This analysis thus suggests that their model is applicable mainly on the landscape level. On the level of individual habitats, the applicability of their results depends on the habitat configuration (at its best for the prevailing habitat of the landscape and for moderate-sized patches of an essentially more productive habitat) and generally decreases with decreasing patch sizes. 相似文献
94.
Mursu J Voutilainen S Nurmi T Alfthan G Virtanen JK Rissanen TH Happonen P Nyyssönen K Kaikkonen J Salonen R Salonen JT 《Free radical biology & medicine》2005,38(4):527-534
Despite extensive research, the cardiovascular effects of coffee consumption in humans remain controversial. Our aim was to investigate the excretion of coffee phenols and the effects of filtered coffee consumption on oxidative stress and plasma homocysteine (tHcy) concentration in humans. The study consisted of a multiple-dose clinical supplementation trial and a single-dose study. In the long-term trial, 43 healthy nonsmoking men optionally consumed daily either no coffee, 3 cups (450 mL), or 6 cups (900 mL) of filtered coffee for 3 weeks, while in the short-term study 35 subjects consumed a single dose of 0, 1 (150 mL), or 2 cups (300 mL) of coffee. Long-term consumption of coffee increased the urinary excretion of caffeic and ferulic acid. The change in the total excretion of phenolic acids in 3 and 6 cups groups represented 3.8 and 2.5% of the amount ingested daily. Plasma tHcy concentrations increased nonsignificantly, but the consumption of coffee had neither short-nor long-term effects on lipid peroxidation or the activity of measured antioxidant enzymes. In conclusion, the consumption of filtered coffee does not have any detectable effects on lipid peroxidation in healthy nonsmoking men. The effect of coffee consumption on tHcy concentrations needs further investigation. 相似文献
95.
Leskelä T Tilsala-Timisjärvi A Kusnetsov J Neubauer P Breitenstein A 《Journal of microbiological methods》2005,62(2):167-179
The aim of this study was to develop a sensitive, cultivation-independent analytical method for Legionella in man-made water systems which can be performed within one day in crude sample extracts. The new assay for the genus Legionella is a paramagnetic bead based fluorescence sandwich hybridization assay (SHA) for the 16S rRNA based on two oligonucleotide probes which makes the method highly specific. An advantage over RT-PCR is the exclusive detection of viable cells and, due to the high number of 16S RNA molecules, the possibility to apply the method directly in crude cell extracts without prior purification of the nucleic acids. A high sensitivity was obtained by modifying the probe chemistry and hybridization conditions. The most sensitive assay uses a 3'-end biotin-labelled capture probe and a 3'-end DIG tailed detection probe and has a detection limit of 20 amol target molecules corresponding to 1.2x10(7) molecules of 16S rRNA and approximately 1800 Legionella cells. Using this assay type the number of Legionella cells was determined in Legionella contaminated water samples. The results show that the developed SHA can be applied for estimation of the approximate number of Legionella cells based on the number of 16S rRNA molecules in a water sample. 相似文献
96.
Hakanpää J Paananen A Askolin S Nakari-Setälä T Parkkinen T Penttilä M Linder MB Rouvinen J 《The Journal of biological chemistry》2004,279(1):534-539
Hydrophobins are proteins specific to filamentous fungi. Hydrophobins have several important roles in fungal physiology, for example, adhesion, formation of protective surface coatings, and the reduction of the surface tension of water, which allows growth of aerial structures. Hydrophobins show remarkable biophysical properties, for example, they are the most powerful surface-active proteins known. To this point the molecular basis of the function of this group of proteins has been largely unknown. We have now determined the crystal structure of the hydrophobin HFBII from Trichoderma reesei at 1.0 A resolution. HFBII has a novel, compact single domain structure containing one alpha-helix and four antiparallel beta-strands that completely envelop two disulfide bridges. The protein surface is mainly hydrophilic, but two beta-hairpin loops contain several conserved aliphatic side chains that form a flat hydrophobic patch that makes the molecule amphiphilic. The amphiphilicity of the HFBII molecule is expected to be a source for surface activity, and we suggest that the behavior of this surfactant is greatly enhanced by the self-assembly that is favored by the combination of size and rigidity. This mechanism of function is supported by atomic force micrographs that show highly ordered arrays of HFBII at the air water interface. The data presented show that much of the current views on structure function relations in hydrophobins must be re-evaluated. 相似文献
97.
Determination of lignans in human plasma by liquid chromatography with coulometric electrode array detection 总被引:2,自引:0,他引:2
Peñalvo JL Nurmi T Haajanen K Al-Maharik N Botting N Adlercreutz H 《Analytical biochemistry》2004,332(2):384-393
The presence of mammalian lignans, mainly enterolactone, in human plasma has been related to lower incidence of certain cancers and cardiovascular disease. The plant lignans secoisolariciresinol and matairesinol have been reported to be precursors of mammalian lignans, but recently other plant lignans relatively abundant in the diet have also been identified as precursors. To evaluate the importance and contribution of these new dietary precursors to the mammalian lignan formation in vivo, metabolic studies in human subjects must be carried out. For this purpose a method based on high-performance liquid chromatography using coulometric electrode array detection for the simultaneous determination of nine plant lignans and two mammalian lignans in human plasma was developed, validated, and shown to fulfill the reliability criteria. 相似文献
98.
Oestradiol potentiates the effects of certain pyrethroid compounds in the MCF7 human breast carcinoma cell line 总被引:2,自引:0,他引:2
Pyrethroids are the most widely used insecticides for indoor pest control, so human exposure to them is common. The main target of pyrethroids is the nervous system, but their endocrine disrupting capabilities may also be of toxicological concern. In the present study, the proliferation of the breast cancer cell line, MCF7, was studied after a 7-day exposure to various concentrations of pyrethrin, permethrin and cypermethrin. The effects of oestradiol and the combined effects of oestradiol (0.10 nM) and pyrethroids (0.1-100 microM) on MCF7 cell proliferation were also evaluated. Proliferation and cell toxicity were studied by measuring the ATP content with a luminescence method, and mitochondrial metabolic enzyme activity with the WST-1 test. In the ATP test, low concentrations (0.1-1 microM) of pyrethroids in co-exposure with oestradiol caused a clear statistically significant increase in the proliferation of MCF7 cells. This was evident when compared to the proliferative effect caused by 0.1 nM oestradiol alone. High concentrations were cytotoxic, and the greatest cell toxicity was that of cypermethrin, which has a cyano group in its molecular structure. 相似文献
99.
Lise Ruffino Tarja Oksanen Katrine S. Hoset Maria Tuomi Lauri Oksanen Erkki Korpimäki Amandine Bugli Keith A. Hobson Bernt Johansen Aurelia Mäkynen 《Ecography》2016,39(9):871-883
Spatial variation in the strength of trophic cascades in arctic tundra has been related to flows of subsidies across ecosystem boundaries. Here, we ask whether the input of marine subsidies in tundra systems would cause spatial variation in the strength of rodent–plant interactions between coastal areas, where predators have access to marine‐derived resources, and non‐subsidized inland areas of northern Fennoscandia. We present a detailed evaluation of predator–rodent–vegetation interactions along a coast‐inland gradient, during the 2011 rodent outbreak and the two following decline years, by using direct assessments of rodent impacts and tracing of marine‐derived nutrients in the food web. Our results revealed that the main rodent predator during summer, the long‐tailed jaeger Stercorarius longicaudus, did not benefit from marine resources while breeding (relative dietary proportion in chicks’ diet = 0–3%). Contrary to this pattern, parasitic jaegers S. parasiticus, bred exclusively near the coast and preyed effectively on both marine resources (41% of chicks’ diet) and rodents (12%). Mammalian predators also showed a higher activity during winter near the coast. Despite overall higher predator numbers, no evidence was found for lower rodent population growth rates during the three monitoring summers and for weaker rodent grazing impacts in the coastal area. Instead, we documented pronounced damages caused by lemmings and voles on bryophytes and vascular plants, especially dwarf shrubs (e.g. Vaccinum myrtillus) all along the coast–inland gradient. Taken together, our results did not support the hypothesis that marine subsidies would trigger a trophic cascade in coastal tundra areas of northern Fennoscandia during a major rodent outbreak, probably due to a relatively low diversity of marine‐subsidized predators in the region. Comparative observational and experimental studies at large spatial scales in various arctic regions are absolutely necessary for a better understanding of factors causing regional variations in the functioning of arctic food webs. 相似文献
100.
Tarja Rajalahti Chenchen Lin Svein Are Mjøs Olav Martin Kvalheim 《Metabolomics : Official journal of the Metabolomic Society》2016,12(5):81