Cytometric study of the effects of destruxin E on leukemic cells in mice

The activity of destruxin E, a cryptogamic toxin isolated from the hyphomycete Metarhizium anisopliae, was studied on mouse leukemia cells (L 1210 and P 388) in culture. Besides a cytotoxic effect, a cytostatic effect (increase of diplo?d cells proportion) was observed with all doses for P 388 (from 10 micrograms/ml to 0.001 microgram/ml) and to a concentration of 0.1 microgram/ml for L 1210. At the lower doses, the effect on cellular DNA content appears distinctly.     

Formation tumorale d’origine parvovirale chezSibine fusca [Lep.: Limacodidae]

Résumé Un état pathologique se traduisant par la formation de masses d’allure tumorale a été observé et étudié chez la larve du lépidoptèreSibine fusca Stoll ravageur des oléagineux en Colombie. La masse tissulaire résultant de la modification de la paroi de l’intestin moyen est formée par l’entassement, sans élimination, des cellules épithéliales activement multipliées sous l’influence simultanée de la régénération cellulaire liée à la fréquence des mues chez cet insecte et de la pathogénèse nucléaire d’unDensovirus. L’étude ultrastructurale montre les lésions virales nucléaires, la multiplication de type et d’intensité anormaux des cellules et la constitution à partir des cellules virosées de la masse d’allure tumorale à conséquence souvent léthale.

---

Summary A pathological state involving tumor-like masses was observed and studied in larvaeSibine fusca Stoll. a pest of oleaginous plants in Colombia. The tissue mass in the midgut is formed by an accumulation of non eliminated epithelial cells which rapidly multiplied under the joint effect of the cellular regeneration for the molting frequent in that insect, and of the nuclear pathogenesis of a densovirosis. Ultrastructural study revealed the nuclear viral lesions, a cell multiplication of abnormal type and frequency as well as the formation from the virus-infected cells of the tumor-like mass often lethal.

     

Dissémination des virus de polyédries par les Éphippigères prédateurs d'insectes

Summary Transmission of polyhedral viruses byEphippiger bitterensis having ingested virus diseasedLymantria dispar larvae was observed in nature and in experiments. The passage of polyhedral bodies in different parts of the gut was studied by histological methods and the effect of the gut juice was found insignificant. These bodies were detected in dejections during 15 days after ingestion of diseased insects. Viruses were shown by electron microscope and the conservation of their pathogenic effect by infection tests. Significantion of these results in epizootiology and in biological control is discussed.

---

---

Ce travail a bénéficié d'une aide de la D.R.M.E.     

Perspectives sur la classification des virus d'invertébrés

Summary After outlining the evolution of the classification of invertebrate viruses the paper emphasizes that the current need is not to develop separately the classification of this group but to make a thorough study of the characteristics of each virus with the object of incorporating it into a general system to be adopted by the Moscow International Microbiological Congress. Proposals were submitted for such inclusions into several systems of general classification which were actually under consideration.

---

---

Rapport présenté au Symposium O.I.L.B. sur le titrage des virus et champignons entomopathogènes, Paris 3–4 mars 1966.     

Hypophyseal pathology in AIDS.

One hundred and eleven pituitary glands of patients (93 males, 18 females; mean age 32 years, 5 months) who died of fully developed AIDS or ARC were examined under light microscopy with the aid of immunohistochemistry. On post mortem (p.m.) examination a wide series of multiorgan alterations was noticed. Microscopically various lesions in both adeno- and neurohypophysis were seen. These ranged from vessel damage to secondaries to systemic infections, neoplasms and functional derangements. Necrotic lumps due to recent infarction could appear in both parts of the gland, while old fibrous scars sustained a previously overcome necrosis. Different pathogens (mainly fungi) could be seen either within the gland or arising from its meningeal surroundings. Examples of tumour pathology were provided by microadenomas, gliosis/gliomas; the frequency of adenomas (11.7%) was similar to that typical of senility. The functional impairment was mainly connected with ACTH cell hyperplasia, which seems in keeping with corticoadrenal or ACTH-receptor damage.