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排序方式: 共有170条查询结果,搜索用时 31 毫秒
101.
102.
Kundelchuk O. P. Tarasenko L. V. Blume Ya. B. 《Russian Journal of Plant Physiology》2002,49(3):381-386
Amiprophosmethyl (APM) is a herbicide acting as a microtubule antagonist. The effect of APM on root development in sensitive and resistant (apm5r) lines of Nicotiana plumbaginifolia was investigated by means of light and immunofluorescent microscopy and by transmission electron microscopy. The treatment of intact roots with APM (0.1–10 M) led to an increase in the root diameter, termed swelling. The root cells in the elongation zone became almost spherical in the APM-sensitive (control) line but retained a cylindrical shape in the apm5r line. After APM treatment, the cell area on longitudinal root sections decreased reliably in the elongation zone but significantly increased in the meristem zone for both lines. A partial destruction of the cortical microtubule network was observed in the apm5r line, and the full degradation of microtubules was evident in the sensitive line. Analysis of the ultrastructure did not reveal any changes in the main cytoplasmic organelles, but showed disturbances of the cell-wall formation. In the meristem zone, some changes in the nucleus structure and the appearance of polynucleate cells were observed. 相似文献
103.
104.
Alexander G. Reznikov Nadezhda D. Nosenko Larisa V. Tarasenko 《The Journal of steroid biochemistry and molecular biology》1999,69(1-6):109-115
Hormonal and neurotransmitter environment of nondifferentiated cells in the developing brain determines many of gender-specific behavioural and neuroendocrine functions. Early postnatal and long-term effects of maternal stress or prenatal glucocorticoid on sex-related peculiarities of the brain morphology, biogenic monoamine turnover, testosterone metabolism, hypothalamic noradrenaline (NA) and adrenocortical responses to an acute stress were studied in Wistar rat offsprings. Maternal stress (1 h immobilization daily for gestational days 15–21) prevented development of sexual dimorphism in neuronal cell nuclei volumes in suprachiazmatic nucleus (SCN) in 10 day old pups. That was associated with a disappearance of male–female differences in NA and 5-hydroxytryptamine turnover in the preoptic area (POA) and dopamine (DA) turnover in the mediobasal hypothalamus (MBH) by decreasing them in male pups. Hydrocortisone acetate (5 mg daily during the last week of pregnancy) produced changes in NA turnover in the POA of males and females which were quite similar to those after maternal stress. Changes in aromatase and 5-reductase activities in the POA of male pups were quite opposite as affected by maternal stress or prenatal glucocorticoid. Sexual differences in 5-reductase activity in the MBH appeared due to its increase in prenatally stressed male pups. In contrast to adult males, in adult females maternal stress did not restrict hypothalamic NA and blood plasma corticosterone response to acute stress (1 h immobilization). Our findings on morphology and functions of gender-related developing brain areas stand in correlation with modifying effects of maternal stress and prenatal glucocorticoid on behavior and neuroendocrine regulations. 相似文献
105.
A. V. Kiselev P. L. Il’ina A. A. Egorova A. N. Baranov I. A. Guryanov N. V. Bayanova I. I. Tarasenko E. A. Lesina G. P. Vlasov V. S. Baranov 《Russian Journal of Genetics》2007,43(6):593-600
Asymmetrical lysine dendrimers are promising as vectors for delivering gene expression constructs into mammalian cells. The condensing, protective, and transfection properties were studied for pentaspherical lysine dendrimer D5 and its analog D5C10, modified with capric acid residues at the outer sphere; in addition, the transfection activity was assayed for complexes DNA-dendrimer-endosomolytic peptide JTS-1. Fatty acid residues incorporated in lysine dendrimers proved to improve their ability to bind DNA, to protect DNA from nuclease degradation, and to ensure its transfer into the nucleus. Peptide JTS-1 introduced in DNA-dendrimer complexes significantly increased their transfection activity. The potentiating effect of JTS-1 was especially high with the DNA-D5C10 complex. An excess of JTS-1 changed the structure of the complexes and reduced their transfection activity. It was assumed that dendrimers D5 and D5C10 are promising vectors for DNA delivery to eukaryotic cells and provide a basis for constructing more refined nonviral module carriers. 相似文献
106.
Neurodynamic parameters of the human brain and their changes depending on the trimester of an individual annual cycle (IAC), which lasts from one birthday to the next, were studied. In the course of a year, fourfold examination (in February, April, July, and October) of 124 men and 242 women aged from 18 to 20 years was carried out. According to psychological characteristics, the examined subjects were divided into introverts and extroverts. The strength and mobility of nervous processes in extrovert men tended to decrease in the fourth trimester of the IAC, simultaneously with a decrease in the overall state of health. Introvert men exhibited a lower brain efficiency than extrovert men; changes in their brain functional mobility during an IAC were more pronounced than in the case of extrovert men; the lowest parameters were recorded in the second trimester. Annual changes in neurodynamic parameters in women were smaller than in men. 相似文献
107.
108.
T A Deviatkina L M Tarasenko L E Bobyreva N G Sergienko O N Voskresenski? 《Biulleten' eksperimental'no? biologii i meditsiny》1985,100(10):412-414
The experiments on rats have shown that during prolonged subliminal electrostimulation of limbic brain system structures peroxidation is enhanced in the blood and tissues (myocardium, liver, parodontium), with it being realized via activation of sympathoadrenal and hypothalamo-hypophyseal systems and stipulated by the failure of physiologic antioxidative mechanisms. 相似文献
109.
110.
V. F. Tarasenko E. Kh. Baksht A. G. Burachenko I. D. Kostyrya D. V. Rybka 《Plasma Physics Reports》2013,39(7):592-599
The influence of the cathode design on the energy of the main group of electrons generated during a subnanosecond breakdown in atmospheric-pressure air was studied experimentally. The electron energy was measured using a time-of-flight spectrometer with a picosecond time resolution. It is shown that the energy of the main group of electrons increases with increasing cathode curvature radius. It is established using 400- to 650-μm-thick aluminum foils that the electron energy reaches its maximum value in voltage pulses with abrupt trailing edges and amplitudes below the maximum amplitude. Electrons with maximum energies are generated with a stronger spatial and amplitude scatter than those with average energies. 相似文献