Hyperlipidemic rabbit serum reduces recovery of acidic glycosaminoglycans from tissue culture medium

Hyperlipidemic rabbit serum and its lipid extract were found to impair the precipitation of glycosaminoglycans by cetylpyridinium chloride. The inhibition was 60–80% in the case of sulfated glycosaminoglycans and 75–85% in the case of hyaluronic acid. The interfering compounds could be removed by extracting the samples twice with a six-fold volume of ethanol-ether (2:1) for 1 h.     

Offspring Defence in Relation to Litter Size and Age: Experiment in the Bank vole Clethrionomys glareolus

According to parental investment theory, nest defence activity should be related to the reproductive value of the offspring. Alternative hypotheses suggest that defence activity may, for example, depend upon the conspicuousness of the young. Studies concerning this topic have been carried out almost exclusively on birds and experimental data on the diversity of organisms is lacking. Bank voles Clethrionomys glareolus were used to study the effects of the number and age of offspring upon the pup defence activity of mothers. Male bank voles are infanticidal and thus an adult male was used as a predator. Defence trials were conducted in the laboratory and filmed for subsequent analysis. Litter sizes were divided into three treatment groups: reduced (−2 pups), control (±0 pups) and enlarged (+2 pups). In order to study the effect of offspring age upon maternal defence activity the trials were conducted twice: when the pups were 3 and 8 days old. Defence activity increased with the number of offspring and enlarged litters were most actively defended. This result supports parental investment theory and conclusions drawn by earlier studies of birds. However, in contrast to the conclusions of earlier studies, older offspring were defended less than the younger ones. Whilst new-born pups are totally defenceless against predators their vulnerability decreases as they age. Therefore, we suggest that maternal aggression in female bank voles is related to the value as well as to the vulnerability of the offspring. The validity of this explanation and the determinants of parental investment decisions in small mammals in general deserve further study. This revised version was published online in July 2006 with corrections to the Cover Date.     

Comments on the structure and synthesis of jasminol,A triterpene reported from Jasminum Auriculatum

The assignment of the lup-20(29)en-28-ol structure for jasminol, and subsequent synthetic proof, are shown to be insecure.