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排序方式: 共有1725条查询结果,搜索用时 15 毫秒
991.
992.
Tania Francisco Tony A. Rodrigues Marta O. Freitas Cláudia P. Grou Andreia F. Carvalho Clara Sá-Miranda Manuel P. Pinto Jorge E. Azevedo 《The Journal of biological chemistry》2013,288(40):29151-29159
Peroxisomal matrix proteins are synthesized on cytosolic ribosomes and post-translationally targeted to the organelle by PEX5, the peroxisomal shuttling receptor. The pathway followed by PEX5 during this process is known with reasonable detail. After recognizing cargo proteins in the cytosol, the receptor interacts with the peroxisomal docking/translocation machinery, where it gets inserted; PEX5 is then monoubiquitinated, extracted back to the cytosol and, finally, deubiquitinated. However, despite this information, the exact step of this pathway where cargo proteins are translocated across the organelle membrane is still ill-defined. In this work, we used an in vitro import system to characterize the translocation mechanism of a matrix protein possessing a type 1 targeting signal. Our results suggest that translocation of proteins across the organelle membrane occurs downstream of a reversible docking step and upstream of the first cytosolic ATP-dependent step (i.e. before ubiquitination of PEX5), concomitantly with the insertion of the receptor into the docking/translocation machinery. 相似文献
993.
Lucia Mariano da Rocha Silla Alice Zelmanowicz Ingrid Mito Mariana Michalowski Tania Hellwing Marco Antonio Shilling Jo?o Ricardo Friedrisch Christina M. Bittar Cristina Arthmar Mentz Albrecht Elaine Scapinello Claudia Conti Marcia Arthmar Mentz Albrecht Letícia Baggio Annelise Pezzi Bruna Amorin Vanessa Valim Laura Fogliatto Alessandra Paz Claudia Astigarraga Rosane Isabel Bittencourt Gustavo Fischer Liane Daudt 《PloS one》2013,8(7)
This population-based study was designed to detect the prevalence of anemia in a healthy population of children (18 months to 7 years) and women (14 to 30 years) tested in 2006–2007 in the state of Rio Grande do Sul, Brazil as part of an effort to tackle this massive problem that still affects so many people in the XXI century. Anemia was defined according to the WHO. Capillary blood was measured and socioeconomic status was determined according to the Brazilian Association of Market Research Agencies. The median prevalence of anemia in 2198 children was 45.4% and in 1999 women 36.4%. Anemia decreased with age during childhood; although significantly more prevalent in lower classes individuals, it was also high in the upper classes. There are indirect evidences that the lack of iron supplementation and/or iron fortified food may play a role in it. Professionals and society wise measures of education have to be implemented in order to address possible biologic factors involved in childhood psychosocial development in southern Brazil. 相似文献
994.
Wendy Espinosa-Hernández Dora Velez-Uriza Jesús Valdés Cristina Vélez-Del Valle Juan Salas-Benito Rebeca Martínez-Contreras Matilde García-Espítia Mariana Salas-Benito Tania Vega-Almeida Mónica De Nova-Ocampo 《PloS one》2014,9(11)
The 3′ untranslated region (3′UTR) of human astroviruses (HAstV) consists of two hairpin structures (helix I and II) joined by a linker harboring a conserved PTB/hnRNP1 binding site. The identification and characterization of cellular proteins that interact with the 3′UTR of HAstV-8 virus will help to uncover cellular requirements for viral functions. To this end, mobility shift assays and UV cross-linking were performed with uninfected and HAstV-8-infected cell extracts and HAstV-8 3′UTR probes. Two RNA-protein complexes (CI and CII) were recruited into the 3′UTR. Complex CII formation was compromised with cold homologous RNA, and seven proteins of 35, 40, 45, 50, 52, 57/60 and 75 kDa were cross-linked to the 3′UTR. Supermobility shift assays indicated that PTB/hnRNP1 is part of this complex, and 3′UTR-crosslinked PTB/hnRNP1 was immunoprecipitated from HAstV-8 infected cell-membrane extracts. Also, immunofluorescence analyses revealed that PTB/hnRNP1 is distributed in the nucleus and cytoplasm of uninfected cells, but it is mainly localized perinuclearly in the cytoplasm of HAstV-8 infected cells. Furthermore, the minimal 3′UTR sequences recognized by recombinant PTB are those conforming helix I, and an intact PTB/hnRNP1-binding site. Finally, small interfering RNA-mediated PTB/hnRNP1 silencing reduced synthesis viral genome and virus yield in CaCo2 cells, suggesting that PTB/hnRNP1 is required for HAstV replication. In conclusion, PTB/hnRNP1 binds to the 3′UTR HAstV-8 and is required or participates in viral replication. 相似文献
995.
996.
997.
Rapid polymerization of actin filament barbed ends generates protrusive forces at the cell edge, leading to cell migration. Two important regulators of free barbed ends, cofilin and Arp2/3, have been shown to work in synergy (net effect greater than additive). To explore this synergy, we model the dynamics of F-actin at the leading edge, motivated by data from EGF-stimulated mammary carcinoma cells. We study how synergy depends on the localized rates and relative timing of cofilin and Arp2/3 activation at the cell edge. The model incorporates diffusion of cofilin, membrane protrusion, F-actin capping, aging, and severing by cofilin and branch nucleation by Arp2/3 (but not G-actin recycling). In a well-mixed system, cofilin and Arp2/3 can each generate a large pulse of barbed ends on their own, but have little synergy; high synergy occurs only at low activation rates, when few barbed ends are produced. In the full spatially distributed model, both synergy and barbed-end production are significant over a range of activation rates. Furthermore, barbed-end production is greatest when Arp2/3 activation is delayed relative to cofilin. Our model supports a direct role for cofilin-mediated actin polymerization in stimulated cell migration, including chemotaxis and cancer invasion. 相似文献
998.
Andressa Alencastre Fuzari Rodrigues Vanessa de Araújo Barbosa José Dilermando Andrade Filho Reginaldo Pe?anha Brazil 《Memórias do Instituto Oswaldo Cruz》2013,108(7):943-946
Cutaneous leishmaniasis (CL) in the state of Rio de Janeiro is sporadic and can
be characterised as a peridomestic transmission that occurs in modified natural
environments. The aim of this work was to study the fauna and ecological
characteristics of sandflies in an environmentally protected area (the State
Park of Serra da Tiririca) within the remnants of the Atlantic Forest in the
municipalities of Niterói and Maricá and their possible relationship with
leishmaniasis. Captures were performed using light traps during the night once a
month for one year in both sylvatic environments and areas surrounding homes
near the park. A total of 1,037 sandflies were captured, belonging to nine
genera and 12 species: Evandromyia tupynambai (34.1%),
Migonemyia migonei (20.6%), Brumptomyia
cunhai (13.8%), Micropygomyia schreiberi (9.7%),
Psathyromyia lanei (6.5%), Brumptomyia
nitzulescui (5.7%), Evandromyia edwardsi
(5.4%), Nyssomyia intermedia (2.8%), Evandromyia
cortelezzii (0.6%), Pintomyia bianchigalatiae
(0.5%), Lutzomyia longipalpis (0.2%) and Sciopemyia
microps (0.1%). Both Mg. migonei and Ny.
intermedia may be acting as vectors of CL in this area. 相似文献
999.
1000.