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Martin Tangney Wilfrid J. Mitchell Michael Dolberg-Rasmussen 《Biotechnology Techniques》1999,13(2):141-144
A new promoter probe plasmid, pMOL618, has been specifically designed to be selective for strong promoter sequences. The plasmid contains two origins of replication which allow it to replicate both in Bacillus subtilis and Escherichia coli, as well as an indicator gene which also functions in both backgrounds. The plasmid is therefore useful in the screening of promoter sequences in both organisms. The stringency of the promoter selection is demonstrated using a known strong promoter. 相似文献