全文获取类型
收费全文 | 13739篇 |
免费 | 974篇 |
国内免费 | 8篇 |
出版年
2021年 | 97篇 |
2020年 | 64篇 |
2019年 | 105篇 |
2018年 | 120篇 |
2017年 | 145篇 |
2016年 | 188篇 |
2015年 | 260篇 |
2014年 | 347篇 |
2013年 | 831篇 |
2012年 | 622篇 |
2011年 | 654篇 |
2010年 | 436篇 |
2009年 | 434篇 |
2008年 | 672篇 |
2007年 | 668篇 |
2006年 | 629篇 |
2005年 | 626篇 |
2004年 | 657篇 |
2003年 | 677篇 |
2002年 | 632篇 |
2001年 | 474篇 |
2000年 | 481篇 |
1999年 | 413篇 |
1998年 | 194篇 |
1997年 | 177篇 |
1996年 | 151篇 |
1995年 | 150篇 |
1994年 | 148篇 |
1993年 | 143篇 |
1992年 | 302篇 |
1991年 | 256篇 |
1990年 | 264篇 |
1989年 | 251篇 |
1988年 | 245篇 |
1987年 | 199篇 |
1986年 | 225篇 |
1985年 | 183篇 |
1984年 | 138篇 |
1983年 | 153篇 |
1982年 | 127篇 |
1981年 | 96篇 |
1980年 | 95篇 |
1979年 | 139篇 |
1978年 | 83篇 |
1977年 | 85篇 |
1976年 | 60篇 |
1975年 | 73篇 |
1974年 | 58篇 |
1973年 | 70篇 |
1972年 | 58篇 |
排序方式: 共有10000条查询结果,搜索用时 765 毫秒
11.
12.
Tatsuya Matsunami Toshihiro Suzuki Yasuo Hisa Kuniaki Takata Tetsuro Takamatsu Masahito Oyamada 《Cell communication & adhesion》2006,13(1):93-102
To elucidate the role of the spiral limbus in glucose transport in the cochlea, we analyzed the expression and localization of GLUT1, connexin26, connexin30, and occludin in the spiral limbus of the rat cochlea. GLUT1 and occludin were detected in blood vessels. GLUT1, connexin26, connexin30, and occludin were also expressed in fibrocytes just basal to the supralimbal lining cells. Connexin26 and connexin30 were present among not only these GLUT1-positive fibrocytes but also GLUT1-negative fibrocytes. In vivo glucose imaging using 6-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-6-deoxyglucose (6-NBDG, MW 342) together with Evans Blue Albumin (EBA, MW 68,000) showed that 6-NBDG was rapidly distributed throughout the spiral limbus, whereas EBA was localized only in the vessels. Moreover, the gap junctional uncoupler heptanol inhibited the distribution of 6-NBDG. These findings suggest that gap junctions play an important role in glucose transport in the spiral limbus, i.e., that gap junctions mediate glucose transport from GLUT1-positive fibrocytes to GLUT1-negative fibrocytes in the spiral limbus. 相似文献
13.
Yoshinari Tanaka 《Evolution; international journal of organic evolution》1996,50(2):512-523
Social selection is presented here as a parallel theory to sexual selection and is defined as a selective force that occurs when individuals change their own social behaviors, responding to signals sent by conspecifics in a way to influence the other individuals' fitness. I analyze the joint evolution of a social signal and behavioral responsiveness to the signal by a quantitative-genetic model. The equilibria of average phenotypes maintained by a balance of social selection and natural selection and their stability are examined for two alternative assumptions on behavioral responsiveness, neutral and adaptive. When behavioral responsiveness is neutral on fitness, a rapid evolution by runaway selection occurs only with enough genetic covariance between the signal and responsiveness. The condition for rapid evolution also depends on natural selection and the number of interacting individuals. When signals convey some information on signalers (e.g., fighting ability), behavioral responsiveness is adaptive such that a receiver's fitness is also influenced by the signal. Here there is a single point of equilibrium. The equilibrium point and its stability do not depend on the genetic correlation. The condition needed for evolution is that the signal is beneficial for receivers, which results from reliability of the signal. Frequency-dependent selection on responsiveness has almost no influence on the equilibrium and the rate of evolution. 相似文献
14.
15.
16.
17.
18.
A new phosphorylating agent, S-(N-monomethoxytritylaminoethyl)-O-(O-chlorophenyl) phosphorothioate, was prepared and reacted with a 5'-hydroxyl group of an oligonucleotide using 1-mesitylene-sulfonyl-3-nitrotriazole (MSNT) as a condensing agent. After base labile protecting groups were removed, the partially deprotected oligonucleotide was separated on a reversed phase column and converted to the oligonucleotide with an aminoethyl or a phosphoryl group at the 5'-end by treatment with 80% acetic acid or iodine-water, respectively. The syntheses of ppT, pppT, A5'pp5'T and A5'ppp5'T were also performed by treatment of 5'-O-(N-monomethoxytritylaminoethylthiophosphoryl) thymidine with tri-n-octylammonium salt of phosphoric acid, pyrophosphoric acid, pA and ppA, respectively. 相似文献
19.
20.
Madhusoodana P. Nambiar Tatsuya Oda Chaohua Chen Yasuo Kuwazuru Henry C. Wu 《Journal of cellular physiology》1993,154(2):222-228
The intracellular pathway following receptor-mediated endocytosis of cholera toxin was studied using brefeldin A (BFA), which inhibited protein secretion and induced dramatic morphological changes in the Golgi region. In both mouse Y1 adrenal cells and CHO cells, BFA at 1 μg/ml caused a 80–90% inhibition of the cholera toxin (CT)-elevation of intracellular cAMP. The inhibition of the cytotoxicity of CT by BFA was also observed in a rounding assay of Y1 adrenal cells. The inhibition of CT cytotoxicity by BFA was dose dependent, with the ID50 value similar to the LD50 of BFA in Y1 adrenal cells. Binding and internalization of [125I]-cholera toxin in Y1 adrenal cells was not affected by BFA. Unlike the BFA-sensitive cell lines such as Y1 adrenal and CHO cells, BFA at 1 μg/ml did not inhibit the cytotoxicity of CT in PtK1 cells, of which the Golgi structure was BFA-resistant. These results strongly suggest that a BFA-sensitive Golgi is required for the protection of CT cytotoxicity by BFA. In contrast, elevation of the intracellular cAMP by forskolin, which acts directly on the plasma membrane adenylate cyclase, was not affected by BFA. These observations indicate that the intoxication of target cells by CT requires an intact Golgi region for its intracellular trafficking and/or processing. In this respect, CT shares a common intracellular pathway with ricin, Pseudomonas toxin, and modeccin, even though their structures and modes of action are very different. © 1993 Wiley-Liss, Inc. 相似文献