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111.
David R. Maddison Wendy Moore Michael D. Baker Tana M. Ellis Karen A. Ober Jamie J Cannone Robin R. Gutell 《Zoologica scripta》2009,38(1):43-62
The beetle suborder Adephaga is traditionally divided into two sections on the basis of habitat, terrestrial Geadephaga and aquatic Hydradephaga. Monophyly of both groups is uncertain, and the relationship of the two groups has implications for inferring habitat transitions within Adephaga. Here we examine phylogenetic relationships of these groups using evidence provided by DNA sequences from all four suborders of beetles, including 60 species of Adephaga, 4 Archostemata, 3 Myxophaga, and 10 Polyphaga. We studied 18S ribosomal DNA and 28S ribosomal DNA, aligned with consideration of secondary structure, as well as the nuclear protein-coding gene wingless . Independent and combined Bayesian, likelihood, and parsimony analyses of all three genes supported placement of Trachypachidae in a monophyletic Geadephaga, although for analyses of 28S rDNA and some parsimony analyses only if Coleoptera is constrained to be monophyletic. Most analyses showed limited support for the monophyly of Hydradephaga. Outside of Adephaga, there is support from the ribosomal genes for a sister group relationship between Adephaga and Polyphaga. Within the small number of sampled Polyphaga, analyses of 18S rDNA, wingless , and the combined matrix supports monophyly of Polyphaga exclusive of Scirtoidea. Unconstrained analyses of the evolution of habitat suggest that Adephaga was ancestrally aquatic with one transition to terrestrial. However, in analyses constrained to disallow changes from aquatic to terrestrial habitat, the phylogenies imply two origins of aquatic habit within Adephaga. 相似文献
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Elizabeth R. Greco Anish Engineer Tana Saiyin Xiangru Lu MengQi Zhang Douglas L. Jones Qingping Feng 《Journal of cellular and molecular medicine》2022,26(11):3223
Maternal cigarette smoking is a risk factor for congenital heart defects (CHDs). Nicotine replacement therapies are often offered to pregnant women following failed attempts of smoking cessation. However, the impact of nicotine on embryonic heart development is not well understood. In the present study, the effects of maternal nicotine exposure (MNE) during pregnancy on foetal heart morphogenesis were studied. Adult female mice were treated with nicotine using subcutaneous osmotic pumps at 0.75 or 1.5 mg/kg/day and subsequently bred with male mice. Our results show that MNE dose‐dependently increased CHDs in foetal mice. CHDs included atrial and ventricular septal defects, double outlet right ventricle, unguarded tricuspid orifice, hypoplastic left ventricle, thickened aortic and pulmonary valves, and ventricular hypertrophy. MNE also significantly reduced coronary artery size and vessel abundance in foetal hearts. Moreover, MNE resulted in higher levels of oxidative stress and altered the expression of key cardiogenic regulators in the developing heart. Nicotine exposure reduced epicardial‐to‐mesenchymal transition in foetal hearts. In conclusion, MNE induces CHDs and coronary artery malformation in mice. These findings provide insight into the adverse outcomes of foetuses by MNE during pregnancy. 相似文献
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Tana Koudelakova Sarka Bidmanova Pavel Dvorak Antonin Pavelka Radka Chaloupkova Zbynek Prokop Prof. Jiri Damborsky 《Biotechnology journal》2013,8(1):32-45
Haloalkane dehalogenases (EC 3.8.1.5, HLDs) are α/β-hydrolases which act to cleave carbon-halogen bonds. Due to their unique catalytic mechanism, broad substrate specificity and high robustness, the members of this enzyme family have been employed in several practical applications: (i) biocatalytic preparation of optically pure building-blocks for organic synthesis; (ii) recycling of by-products from chemical processes; (iii) bioremediation of toxic environmental pollutants; (iv) decontamination of warfare agents; (v) biosensing of environmental pollutants; and (vi) protein tagging for cell imaging and protein analysis. This review discusses the application of HLDs in the context of the biochemical properties of individual enzymes. Further extension of HLD uses within the field of biotechnology will require currently limiting factors – such as low expression, product inhibition, insufficient enzyme selectivity, low affinity and catalytic efficiency towards selected substrates, and instability in the presence of organic co-solvents – to be overcome. We propose that strategies based on protein engineering and isolation of novel HLDs from extremophilic microorganisms may offer solutions. 相似文献
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Nicholas J Bouskill Hsiao Chien Lim Sharon Borglin Rohit Salve Tana E Wood Whendee L Silver Eoin L Brodie 《The ISME journal》2013,7(2):384-394
Global climate models project a decrease in the magnitude of precipitation in tropical regions. Changes in rainfall patterns have important implications for the moisture content and redox status of tropical soils, yet little is known about how these changes may affect microbial community structure. Specifically, does exposure to prior stress confer increased resistance to subsequent perturbation? Here we reduced the quantity of precipitation throughfall to tropical forest soils in the Luquillo Mountains, Puerto Rico. Treatments included newly established throughfall exclusion plots (de novo excluded), plots undergoing reduction for a second time (pre-excluded) and ambient control plots. Ten months of throughfall exclusion led to a small but statistically significant decline in soil water potential and bacterial populations clearly adapted to increased osmotic stress. Although the water potential decline was small and microbial biomass did not change, phylogenetic diversity in the de novo-excluded plots decreased by ∼40% compared with the control plots, yet pre-excluded plots showed no significant change. On the other hand, the relative abundances of bacterial taxa in both the de novo-excluded and pre-excluded plots changed significantly with throughfall exclusion compared with control plots. Changes in bacterial community structure could be explained by changes in soil pore water chemistry and suggested changes in soil redox. Soluble iron declined in treatment plots and was correlated with decreased soluble phosphorus concentrations, which may have significant implications for microbial productivity in these P-limited systems. 相似文献
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Evguenii I. Kozliak Tana L. Ostlie-Dunn Michele L. Jacobson Steven R. Mattson Ryan T. Domack 《Bioremediation Journal》2000,4(1):81-96
Fibers are suggested for bacterial immobilization in trickle-bed bioreactors used for the removal of volatile organic compounds (VOCs) from air. Fiber-based bioreactors retain up to 200 to 300 mg of dry biomass per 1 g of support, which is a much larger value than that of traditional, granule-based bioreactors. Air pollutant removal efficiency for fiber-based bioreactors remains high with large inlet pollutant concentrations or space velocities (lower contact times). Efficient removal is achieved not only for a water-miscible substrate (ethanol), but also for some less water-soluble compounds, such as ethyl acetate and styrene. Specific pollutant elimination capacity per unit fiber-based biocatalyst volume (up to 4000 g/m3-h) exceeds those of biological air purification methods and is comparable to chemical methods. Unlike granule-based biocatalysts, oxygen limitation for pollutant biodegradation is not observed. Evidence obtained shows that the higher air purification efficiency is due to the greater surface-to-volume ratio of fibers when compared with granules, which results in a more efficient substrate mass transfer. 相似文献