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91.
Cerebral spinal fluid (CSF) and structural imaging markers are suggested as biomarkers amended to existing diagnostic criteria of mild cognitive impairment (MCI) and Alzheimer''s disease (AD). But there is no clear instruction on which markers should be used at which stage of dementia. This study aimed to first investigate associations of the CSF markers as well as volumes and shapes of the hippocampus and lateral ventricles with MCI and AD at the baseline and secondly apply these baseline markers to predict MCI conversion in a two-year time using the Alzheimer''s Disease Neuroimaging Initiative (ADNI) cohort. Our results suggested that the CSF markers, including Aβ42, t-tau, and p-tau, distinguished MCI or AD from NC, while the Aβ42 CSF marker contributed to the differentiation between MCI and AD. The hippocampal shapes performed better than the hippocampal volumes in classifying NC and MCI, NC and AD, as well as MCI and AD. Interestingly, the ventricular volumes were better than the ventricular shapes to distinguish MCI or AD from NC, while the ventricular shapes showed better accuracy than the ventricular volumes in classifying MCI and AD. As the CSF markers and the structural markers are complementary, the combination of them showed great improvements in the classification accuracies of MCI and AD. Moreover, the combination of these markers showed high sensitivity but low specificity for predicting conversion from MCI to AD in two years. Hence, it is feasible to employ a cross-sectional sample to investigate dynamic associations of the CSF and imaging markers with MCI and AD and to predict future MCI conversion. In particular, the volumetric information may be good for the early stage of AD, while morphological shapes should be considered as markers in the prediction of MCI conversion to AD together with the CSF markers. 相似文献
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93.
Qingqing Feng Lu Han Xu Tan Yali Zhang Tianyi Meng Jun Lu Jie Lv 《Current microbiology》2016,73(6):924-929
94.
Mei-Juan Tan Xi Chen Yu-Kuan Wang Guang-Lei Liu Zhen-Ming Chi 《Bioprocess and biosystems engineering》2016,39(8):1289-1296
In this study, after the expression of a pyruvate carboxylase gene (PYC) cloned from Meyerozyma guilliermondii in a marine-derived yeast Yarrowia lipolytica SWJ-1b, a transformant PG86 obtained had much higher PYC activity than Y. lipolytica SWJ-1b. At the same time, the PYC gene expression and citric acid (CA) production by the transformant PG86 were also greatly enhanced. When glucose concentration in the medium was 60.0 g L?1, CA concentration formed by the transformant PG86 was 34.02 g L?1, leading to a CA yield of 0.57 g g?1 of glucose. During a 10-L fed-batch fermentation, the final concentration of CA was 101.0 ± 1.3 g L?1, the yield was 0.89 g g?1 of glucose, the productivity was 0.42 g L?1 h?1 and only 5.93 g L?1 reducing sugar was left in the fermented medium within 240 h of the fed-batch fermentation. HPLC analysis showed that most of the fermentation products were CA. 相似文献
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96.
Julie Tan 《Critical reviews in biochemistry and molecular biology》2014,49(1):33-58
AbstractPhosphatidylinositol lipids are signaling molecules involved in nearly all aspects of cellular regulation. Production of phosphatidylinositol 4-phosphate (PI4P) has long been recognized as one of the first steps in generating poly-phosphatidylinositol phosphates involved in actin organization, cell migration, and signal transduction. In addition, progress over the last decade has brought to light independent roles for PI4P in membrane trafficking and lipid homeostasis. Here, we describe recent advances that reveal the breadth of processes regulated by PI4P, the spectrum of PI4P effectors, and the mechanisms of spatiotemporal control that coordinate crosstalk between PI4P and cellular signaling pathways. 相似文献
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Phosphorylation of Pkp1 by RIPK4 regulates epidermal differentiation and skin tumorigenesis 下载免费PDF全文
Philbert Lee Shangwen Jiang Yuanyuan Li Jiping Yue Xuewen Gou Shao‐Yu Chen Yingming Zhao Markus Schober Minjia Tan Xiaoyang Wu 《The EMBO journal》2017,36(13):1963-1980
Tissue homeostasis of skin is sustained by epidermal progenitor cells localized within the basal layer of the skin epithelium. Post‐translational modification of the proteome, such as protein phosphorylation, plays a fundamental role in the regulation of stemness and differentiation of somatic stem cells. However, it remains unclear how phosphoproteomic changes occur and contribute to epidermal differentiation. In this study, we survey the epidermal cell differentiation in a systematic manner by combining quantitative phosphoproteomics with mammalian kinome cDNA library screen. This approach identified a key signaling event, phosphorylation of a desmosome component, PKP1 (plakophilin‐1) by RIPK4 (receptor‐interacting serine–threonine kinase 4) during epidermal differentiation. With genome‐editing and mouse genetics approach, we show that loss of function of either Pkp1 or Ripk4 impairs skin differentiation and enhances epidermal carcinogenesis in vivo. Phosphorylation of PKP1's N‐terminal domain by RIPK4 is essential for their role in epidermal differentiation. Taken together, our study presents a global view of phosphoproteomic changes that occur during epidermal differentiation, and identifies RIPK‐PKP1 signaling as novel axis involved in skin stratification and tumorigenesis. 相似文献
100.
In this paper, photosynthetic characteristics of green leaves (GL) and green pseudobulbs (GPSB) of C3 orchid Oncidium Golden Wish were first studied. Light saturation for photosynthesis and maximum photosynthetic rates (P
max) were significantly higher in GL than in GPSB. The results of the optimal PSII quantum yield (Fv/Fm ratio), electron transport rate (ETR), the effective photochemical quantum yield (ΔF/Fm′) and nonphotochemical quenching (NPQ) of Chl fluorescence revealed that GPSB had lower light utilization than that of GL.
Significantly higher photosynthetic pigments were found in GL than in GPSB. Alteration of source/sink ratio had no impact
on all photosynthetic parameters for both GL and GPSB after a short term of 3 days or even a long term of 2 weeks of treatments
although there were significant decreases in GL carbohydrate concentration of GL-darkened plants by the end of the day. However,
decreases of all photosynthetic parameters of GL were observed in GL-darkened plants after 4 weeks of treatment compared to
those of fully illuminated (FI) and GPSB-darkened plants. These results indicate that the level of carbohydrates in GL plays
an important role in regulating their photosynthesis. Due to their lower photosynthetic capacities, GPSB function mainly as
sinks. Darkening GPSB up to 2 weeks did not affect their own P
max and the P
max of GL and thus, did not result in significant decreases of total carbohydrate concentration of GPSB. As GPSB store a large
amount of carbohydrates, it could also act as a source when the level of carbohydrates decreased. Thus, GL could depend on
GPSB carbohydrates to regulate their photosynthesis when their source capacity was removed. However, 4 weeks after treatments,
photosynthetic capacities of GL were significantly lower in GL- and GPSB-darkened plants than in FI plants, which could be
due to the lower total soluble and insoluble sugar concentrations of both GL and GPSB in these plants. 相似文献