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71.
Synchronization of cell death in a dinoflagellate population is mediated by an excreted thiol protease 总被引:3,自引:0,他引:3
Vardi A Eisenstadt D Murik O Berman-Frank I Zohary T Levine A Kaplan A 《Environmental microbiology》2007,9(2):360-369
Regulated programmed cell death (PCD) processes have been documented in several phytoplankton species and are hypothesized to play a role in population dynamics. However, the mechanisms leading to the coordinated collapse of phytoplankton blooms are poorly understood. We showed that the collapse of the annual bloom of Peridinium gatunense, an abundant dinoflagellate in Lake Kinneret, Israel, is initiated by CO2 limitation followed by oxidative stress that triggers a PCD-like cascade. We provide evidences that a protease excreted by senescing P. gatunense cells sensitizes younger cells to oxidative stress and may consequently trigger synchronized cell death of the population. Ageing of the P. gatunense cultures was characterized by a remarkable rise in DNA fragmentation and enhanced sensitivity to H2O2. Exposure of logarithmic phase (young) cultures to conditioning media from stationary phase (old) cells sensitized them to H2O2 and led to premature massive cell death. We detected the induction of specific extracellular protease activity, leupeptin-sensitive, in ageing cultures and in lake waters during the succession of the P. gatunense bloom. Partial purification of the conditioned media revealed that this protease activity is responsible for the higher susceptibility of young cells to oxidative stress. Inhibition of the protease activity lowered the sensitivity to oxidative stress, whereas application of papain to logarithmic phase P. gatunense cultures mimicked the effect of the spent media and enhanced cell death. We propose a novel mechanistic framework by which a population of unicellular phytoplankton orchestrates a coordinated response to stress, thereby determine the fate of its individuals. 相似文献
72.
Non-indigenous species cause great damage worldwide. Non-indigenous insects are known as harmful in many regions, but few
comprehensive works have investigated non-indigenous insects as a group. We compiled a comprehensive database of established
non-indigenous (ENI) insects in Israel and adjacent regions to investigate how they arrived, their biological characteristics,
and the attributes of areas they invade. Of 218 species of ENI insects in this region, 124 are widespread. Most species came
as stowaways, but 38 were brought intentionally for biological control. Most ENI insects in this region are in the Coleoptera,
Diptera, Hymenoptera, Lepidoptera, and Homoptera. Species from various orders differ in their tendency to be localized or
widespread, and in biogeographic origins. The distribution of species among orders differs between native and ENI insects.
The Coastal Plain houses the most ENI insect species and the Negev and Judean deserts the fewest. Most ENI insects spread
from the Coastal Plain to other regions. Absence of roads, settlements and presence of nature reserves are negatively correlated
with occurrence of ENI species. Seventy-nine species are categorized as pests that damage produce, merchandise, forestry,
etc. Despite a general dearth of knowledge on impacts of ENI insects on natural systems, 42 species are known to feed on native
plants, some of conservation concern. Biological control agents are usually more limited in their distribution than other
ENI insects. Further research, legislation, and enforcement are required to minimize effects of these species on agriculture
and natural habitats. 相似文献
73.
Follicular dendritic cell sarcomas (FDCS) are rare tumours of lymph nodes and extranodal tissues which are grouped with the histiocytic and dendritic cell neoplasms. The diagnosis is usually made after thorough clinical and pathological examination with immunohistochemical analysis. Difficulties persist in diagnosing FDCS on cytological preparations. We report herein a case of a 57-year-old female who presented with a right neck mass of 5 months duration. Computed Tomography (CT) imaging of the neck reported a necrotic right level IIb lymph node and asymmetric fullness of the right palatine tonsil. Fine needle aspiration (FNA) biopsy revealed numerous spindle, oval and stellate neoplastic cells, arranged singly and in syncytia with moderate nuclear pleomorphism, vesicular chromatin pattern, and prominent nucleoli, sprinkled with small lymphocytes. The tumour cells were strongly diffusely positive for CD21, CD23, and D2-40 immunostaining on cell bock sections, but were negative for CD1a and CD34, supporting the diagnosis of FDCS. Follow-up surgical pathology on the resection showed histopathological features and an immunohistochemical profile consistent with FDCS. 相似文献
74.
Mai Zahran Cigdem Sevim?Bayrak Shereef Elmetwaly Tamar Schlick 《Nucleic acids research》2015,43(19):9474-9488
To address many challenges in RNA structure/function prediction, the characterization of RNA''s modular architectural units is required. Using the RNA-As-Graphs (RAG) database, we have previously explored the existence of secondary structure (2D) submotifs within larger RNA structures. Here we present RAG-3D—a dataset of RNA tertiary (3D) structures and substructures plus a web-based search tool—designed to exploit graph representations of RNAs for the goal of searching for similar 3D structural fragments. The objects in RAG-3D consist of 3D structures translated into 3D graphs, cataloged based on the connectivity between their secondary structure elements. Each graph is additionally described in terms of its subgraph building blocks. The RAG-3D search tool then compares a query RNA 3D structure to those in the database to obtain structurally similar structures and substructures. This comparison reveals conserved 3D RNA features and thus may suggest functional connections. Though RNA search programs based on similarity in sequence, 2D, and/or 3D structural elements are available, our graph-based search tool may be advantageous for illuminating similarities that are not obvious; using motifs rather than sequence space also reduces search times considerably. Ultimately, such substructuring could be useful for RNA 3D structure prediction, structure/function inference and inverse folding. 相似文献
75.
Isadora S. Cohen Carmit Bar Tamar Paz-Elizur Elena Ainbinder Karoline Leopold Niels de?Wind Nicholas Geacintov Zvi Livneh 《Nucleic acids research》2015,43(3):1637-1645
DNA-damage tolerance (DDT) via translesion DNA synthesis (TLS) or homology-dependent repair (HDR) functions to bypass DNA lesions encountered during replication, and is critical for maintaining genome stability. Here, we present piggyBlock, a new chromosomal assay that, using piggyBac transposition of DNA containing a known lesion, measures the division of labor between the two DDT pathways. We show that in the absence of DNA damage response, tolerance of the most common sunlight-induced DNA lesion, TT-CPD, is achieved by TLS in mouse embryo fibroblasts. Meanwhile, BP-G, a major smoke-induced DNA lesion, is bypassed primarily by HDR, providing the first evidence for this mechanism being the main tolerance pathway for a biologically important lesion in a mammalian genome. We also show that, far from being a last-resort strategy as it is sometimes portrayed, TLS operates alongside nucleotide excision repair, handling 40% of TT-CPDs in repair-proficient cells. Finally, DDT acts in mouse embryonic stem cells, exhibiting the same pattern—mutagenic TLS included—despite the risk of propagating mutations along all cell lineages. The new method highlights the importance of HDR, and provides an effective tool for studying DDT in mammalian cells. 相似文献
76.
Experimental management control of Opuntia dillenii Haw. and Agave americana L. in Teno Rural Park,Canary Islands
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José R. Arévalo Silvia Fernández‐Lugo Miguel Mellado Tamar de la Concepción 《Plant Species Biology》2015,30(2):137-146
Invasion biology is an important element of global environmental change and represents one of the main threats to biodiversity. American species were introduced to Tenerife after the Spanish conquest during the eighteenth century, as is the case for Agave americana and Opuntia dillenii. The long period of naturalization and adaptation of these species has led them to become two of the most dispersed introduced species of the archipelago. We analyzed several eradication management processes in an area intensively invaded by both O. dillenii and A. americana. Three treatments were randomly applied: mechanical removal, use of herbicide (glyphosate at 10% volume), and mechanical and herbicide applied together. Both the effectiveness of the treatments to remove the target exotic species biovolume and the impact of the eradication methods on species richness and species composition of the area were analyzed. We found that the treatments had an impact on species composition but not on species richness. Species composition was mainly affected by mechanical treatment. The effect caused by the mechanical removal of the exotic target species in species composition is minor after 4 years, and is related to a higher dominance of shrub species typical of coastal shrubland and of annual or pioneer species. The control of O. dillenii and A. americana is evident from insignificant recovery 4 years after treatment application. A mechanical and herbicide treatment together, allowed not only the immediate removal of large individuals but also the herbicidal control of smaller ones. 相似文献
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80.
Siton O Ideses Y Albeck S Unger T Bershadsky AD Gov NS Bernheim-Groswasser A 《Current biology : CB》2011,21(24):2092-2097
Cortactin is involved in invadopodia and podosome formation [1], pathogens and endosome motility [2], and persistent lamellipodia protrusion [
[3]
and
[4]
]; its overexpression enhances cellular motility and metastatic activity [
[5]
,
[6]
,
[7]
and
[8]
]. Several mechanisms have been proposed to explain cortactin's role in Arp2/3-driven actin polymerization [
[9]
and
[10]
], yet its direct role in cell movement remains unclear. We use a biomimetic system to study the mechanism of cortactin-mediated regulation of actin-driven motility [11]. We tested the role of different cortactin variants that interact with Arp2/3 complex and actin filaments distinctively. We show that wild-type cortactin significantly enhances the bead velocity at low concentrations. Single filament experiments show that cortactin has no significant effect on actin polymerization and branch stability, whereas it strongly affects the branching rate driven by Wiskott-Aldrich syndrome protein (WASP)-VCA fragment and Arp2/3 complex. These results lead us to propose that cortactin plays a critical role in translating actin polymerization at a bead surface into motion, by releasing WASP-VCA from the new branching site. This enhanced release has two major effects: it increases the turnover rate of branching per WASP molecule, and it decreases the friction-like force caused by the binding of the moving surface with respect to the growing actin network. 相似文献