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101.
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The purpose of this study was to investigate the limitation for applying a linear model to the cardiorespiratory control system. Four subjects performed the two types of exercise bouts, constant (CONST) and pseudorandom (PRBS) exercise, on an electrically braked cycle ergometer at three different work rates. The target work rate of CONST were set to 80, 100, 120% of the individual anaerobic threshold (AT). In PRBS, the work rates were varied between +/- 10% of the individual AT around the respective target work rates of CONST. Although the spectral density of beat-to-beat heart rate fluctuations showed the conventional patterns for most cases, there was no obvious difference between CONST and PRBS. These results indicated that the variation of +/- 10% of AT did not affect the heart rate variation as the output response, suggesting a dilemma inevitable to apply a linear model based on the transfer function. 相似文献
104.
105.
Kentabo Tanaka Mikio Tamaki Takuzì Itoh Eiko Otaka Syozo Osawa 《Molecular & general genetics : MGG》1972,114(1):23-30
Summary Mutants from Escherichia coli Q13 were selected for resistance to leucomycin, tylosin or spiramycin. Most of the mutants so selected exhibited cross resistance to all the macrolide antibiotics tested including erythromycin. A few mutants however seem to be less resistant to erythromycin. One mutant, QSP008, was highly resistant to tylosin, leucomycin and spiramycin but relatively sensitive to erythromycin. Another mutant, QSP006, was highly resistant to spiramycin but less resistant to erythromycin, tylosin and leucomycin. This selective resistance of cells to specific antibiotics could be due to the extent of conformational alteration of their ribosomes, which may be demonstrated by the extent of 14C-erythromycin binding to these ribosomes. The ribosomes from QSP008 cells were found to contain an altered 50-8 protein of the 50s ribosomal subunit, while in the ribosomes from QSP006 no such protein change could be detected by the methods used.A preliminary data of part of this work has been published (Tanaka, Teraoka, Tamaki, Watanabe, Osawa, Otaka, and Takata, 1971). 相似文献
106.
Kentaro Tanaka Hiroshi Teraoka Mikio Tamaki Renkichi Takata Syozo Osawa 《Molecular & general genetics : MGG》1972,114(1):9-13
Summary An erythromycin resistant (ery
r) mutant of Escherichia coli Q13, QE107, was characterized by (1) the cross resistance of the cells to several macrolide antibiotics such as erythromycin, tylosin, spiramycin, oleandomycin and leucomycin, (2) the reduced affinity of its ribosomes to erythromycin and probably to the other macrolides mentioned above, (3) a low peptidyl transferase activity of its ribosomes and (4) an altered 50-8 protein of the 50s ribosomal subunits. These characters were always transferred together with the ery marker in the transduction experiments.Preliminary data of part of this work has been published (Tanaka, Teraoka, Tamaki, Watanabe, Osawa, Otaka and Takata, 1971). 相似文献
107.
Hajime Matsushita M. Noguchi E. Tamaki 《Biochemical and biophysical research communications》1974,57(4):1006-1010
The preparation and characterization of a nicotine-albumin conjugate are reported. The nicotine derivative, 6-(p-aminobenzamido)nicotine, which was coupled to bovine serum albumin(BSA), was synthesized by the following sequence; -nicotine→6-aminonicotine→6-(p-nitrobenzamido)nicotine→6-(p-aminobenzamido)nicotine. Thirty molecules of conjugated nicotine per molecule of BSA were distinguished by the determination of 6-aminonicotine after hydrolysis. The distribution of the conjugated nicotine on BSA was examined by the stoichiometry of the composed amino acids before and after conjugation, and also by the electrophoretic behavior of the conjugated BSA on a polyacrylamide gel. 相似文献
108.
Erythromycin resistant mutants of Bacillus subtilis 总被引:6,自引:0,他引:6
K. Tanaka M. Tamaki S. Osawa A. Kimura R. Takata 《Molecular & general genetics : MGG》1973,127(2):157-161
Summary Erythromycin resistant (ery
r) mutants were isolated from Bacillus subtilis ATCC 6633. The composition of ribosomal proteins were analyzed for thirteen such ery
r-mutants with chromatography on a carboxymethyl cellulose (CMC) column. The 50s subunit from all of the ery
r-mutants was found to contain the altered 50d protein. The ribosomes prepared from the ery
r-mutants did not show in vitro alteration of the ability to combine with erythromycin. 相似文献
109.
Biochemical and genetic studies on two different types of erythromycin resistant mutants of Escherichia coli with altered ribosomal proteins 总被引:22,自引:0,他引:22
H. G. Wittmann G. Stöffler D. Apirion L. Rosen K. Tanaka M. Tamaki R. Takata S. Dekio E. Otaka S. Osawa 《Molecular & general genetics : MGG》1973,127(2):175-189
Summary Ribosomes from nine E. coli mutants with high level resistance to the antibiotic erythromycin were isolated and their proteins were compared with those of the parental strains by two-dimensional polyacrylamide gel electrophoresis, by carboxymethylcellulose column chromatography and by immunological techniques. Two 50S proteins were found to be altered in the mutants: either L 4 or L 22.Ribosomes with an altered L4 protein bound erythromycin rather poorly and the formation of N-acetylphenylalanyl puromycin was drastically decreased. On the other handribosomes with an altered L22 protein bound erythromycin as efficiently as wild type ribosomes and their puromycin reaction was at least as good as that of wild type ribosomes.Transduction experiments showed that the mutations affecting both proteins, L4 and L22, are located very close to the str and spc genes, nearer to the spc than to str gene.Paper No. 61 on Ribosomal Proteins. Preceding paper is by Hasenbank et al., Molec. gen. Genet., 127, 1–18 (1973).Communicated by E. Bautz 相似文献
110.
Mizusaki Shigenobu; Tanabe Yoko; Noguchi Masao; Tamaki Einosuke 《Plant & cell physiology》1973,14(1):103-110
Changes in ornithine decarboxylase, putrescine N-methyltransferaseand N-methylputrescine oxidase activities in response to sometreatments were investigated using hydroponically grown tobaccoplants. Decapitation of shoots brought about marked elevationof the three enzyme activities, which reached their peaks 24hr after decapitation, then declined. An excellent correlationwas observed between the accumulation of nicotine and changesin the three root enzyme activities. Administration of IAA at2.5 to 5 µconcentration significandy increased these enzymeactivities in roots of decapitated plants but higher concentrationsof IAA prevented the rise in enzyme activities promoted by decapitation.Nicotine strongly inhibited the rise in enzyme activities inroots of decapitated plants in all cases. The results suggestthat these enzymes are under the control of a common regulatorysystem, in which auxin and nicotine are important components.Ornithine decarboxylase was present in all the plants examined,but putrescine N-methyltransferase and N-mediylputrescine oxidasewere detected only in the roots of tobacco, Datura and Atropaplants.
1Part XVI of the series "Phytochemical Studies on Tobacco Alkaloids". (Received August 1, 1972; ) 相似文献