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101.
Paulo Breinis Flavio Geraldes Alves Camila AE Alves Rafael G Cintra Débora Almeida Priscila C Passarelli Camila Domingues Talita Gerbim Régia Gasparetto Luiz Carlos de Abreu Vitor E Valenti Adriana Gonçalves de Oliveira Carlos Bandeira de Mello Monteiro Rubens Wajnzstejn 《BMC neurology》2014,14(1):1-4
Background
The Mulvihill-Smith Syndrome was first recognized in 1975. After the recognition of the Mulvihill-Smith Syndrome, ten cases have been described.Case presentation
This article describes the eleventh case of this syndrome in a male patient, 24 years-old with short stature and microcephaly with mild cognitive impairment, deafness and allergic conjunctivitis. The patient was hospitalized several times for repeated infections, and the presence of multiple melanocytic nevi on his skin was noticed.Conclusions
Based on the entire set of signs and symptoms presented in our study, it was diagnosed the patient with Mulvihill-Smith Syndrome. 相似文献102.
Janaína Garcia Gon?alves Ana Carolina de Bragan?a Daniele Canale Maria Heloisa Massola Shimizu Talita Rojas Sanches Rosa Maria Affonso Moysés Lúcia Andrade Antonio Carlos Seguro Rildo Aparecido Volpini 《PloS one》2014,9(9)
Background
Despite a significant improvement in the management of chronic kidney disease (CKD), its incidence and prevalence has been increasing over the years. Progressive renal fibrosis is present in CKD and involves the participation of several cytokines, including Transforming growth factor-β1 (TGF-β1). Besides cardiovascular diseases and infections, several studies show that Vitamin D status has been considered as a non-traditional risk factor for the progression of CKD. Given the importance of vitamin D in the maintenance of essential physiological functions, we studied the events involved in the chronic kidney disease progression in rats submitted to ischemia/reperfusion injury under vitamin D deficiency (VDD).Methods
Rats were randomized into four groups: Control; VDD; ischemia/reperfusion injury (IRI); and VDD+IRI. At the 62 day after sham or IRI surgery, we measured inulin clearance, biochemical variables and hemodynamic parameters. In kidney tissue, we performed immunoblotting to quantify expression of Klotho, TGF-β, and vitamin D receptor (VDR); gene expression to evaluate renin, angiotensinogen, and angiotensin-converting enzyme; and immunohistochemical staining for ED1 (macrophages), type IV collagen, fibronectin, vimentin, and α-smooth mucle actin. Histomorphometric studies were performed to evaluate fractional interstitial area.Results
IRI animals presented renal hypertrophy, increased levels of mean blood pressure and plasma PTH. Furthermore, expansion of the interstitial area, increased infiltration of ED1 cells, increased expression of collagen IV, fibronectin, vimentin and α-actin, and reduced expression of Klotho protein were observed. VDD deficiency contributed to increased levels of plasma PTH as well as for important chronic tubulointerstitial changes (fibrosis, inflammatory infiltration, tubular dilation and atrophy), increased expression of TGF-β1 and decreased expression of VDR and Klotho protein observed in VDD+IRI animals.Conclusion
Through inflammatory pathways and involvement of TGF-β1 growth factor, VDD could be considered as an aggravating factor for tubulointerstitial damage and fibrosis progression following acute kidney injury induced by ischemia/reperfusion. 相似文献103.
Kelly Ishida Talita Ferreira Cipriano Gustavo Miranda Rocha Gilberto Weissmüller Fabio Gomes Kildare Miranda Sonia Rozental 《Memórias do Instituto Oswaldo Cruz》2014,109(2):220-228
The microbial synthesis of nanoparticles is a green chemistry approach that
combines nanotechnology and microbial biotechnology. The aim of this study was
to obtain silver nanoparticles (SNPs) using aqueous extract from the filamentous
fungus Fusarium oxysporum as an alternative to chemical
procedures and to evaluate its antifungal activity. SNPs production increased in
a concentration-dependent way up to 1 mM silver nitrate until 30 days of
reaction. Monodispersed and spherical SNPs were predominantly produced. After 60
days, it was possible to observe degenerated SNPs with in additional needle
morphology. The SNPs showed a high antifungal activity against
Candida and Cryptococcus , with minimum
inhibitory concentration values ≤ 1.68 µg/mL for both genera. Morphological
alterations of Cryptococcus neoformans treated with SNPs were
observed such as disruption of the cell wall and cytoplasmic membrane and lost
of the cytoplasm content. This work revealed that SNPs can be easily produced by
F. oxysporum aqueous extracts and may be a feasible,
low-cost, environmentally friendly method for generating stable and uniformly
sized SNPs. Finally, we have demonstrated that these SNPs are active against
pathogenic fungi, such as Candida and
Cryptococcus . 相似文献
104.
An Evaluation of the Effects of Acute and Chronic l-Tyrosine Administration on BDNF Levels and bdnf mRNA Expression in the Rat Brain 总被引:1,自引:0,他引:1
105.
Raul Bardini Bressan Fernanda Rosene Melo Patricia Alves Almeida Denise Avani Bittencourt Silvia Visoni Talita Silva Jeremias Ana Paula Costa Rodrigo Bainy Leal Andrea Gonçalves Trentin 《Experimental cell research》2014
Epidermal neural crest stem cells (EPI-NCSCs), which reside in the bulge of hair follicles, are attractive candidates for several applications in cell therapy, drug screening and tissue engineering. As suggested remnants of the embryonic neural crest (NC) in an adult location, EPI-NCSCs are able to generate a wide variety of cell types and are readily accessible by a minimally invasive procedure. Since the combination of epidermal growth factor (EGF) and fibroblast growth factor type 2 (FGF2) is mitogenic and promotes the neuronal commitment of various stem cell populations, we examined its effects in the proliferation and neuronal potential of mouse EPI-NCSCs. By using a recognized culture protocol of bulge whiskers follicles, we were able to isolate a population of EPI-NCSCs, characterized by the migratory potential, cell morphology and expression of phenotypic markers of NC cells. EPI-NCSCs expressed neuronal, glial and smooth muscle markers and exhibited the NC-like fibroblastic morphology. The treatment with the combination EGF and FGF2, however, increased their proliferation rate and promoted the acquisition of a neuronal-like morphology accompanied by reorganization of neural cytoskeletal proteins βIII-tubulin and nestin, as well as upregulation of the pan neuronal marker βIII-tubulin and down regulation of the undifferentiated NC, glial and smooth muscle cell markers. Moreover, the treatment enhanced the response of EPI-NCSCs to neurogenic stimulation, as evidenced by induction of GAP43, and increased expression of Mash-1 in neuron-like cell, both neuronal-specific proteins. Together, the results suggest that the combination of EGF–FGF2 stimulates the proliferation and improves the neuronal potential of EPI-NCSCs similarly to embryonic NC cells, ES cells and neural progenitor/stem cells of the central nervous system and highlights the advantage of using EGF–FGF2 in neuronal differentiation protocols. 相似文献
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108.
Ricardo A. S. Cerboncini Talita V. Braga James J. Roper Fernando C. Passos 《Ibis》2020,162(1):227-231
Cooperative breeding is rare in shorebirds, and when found it is thought to be due to polygamous mating (cooperative polygamy). Here we describe the social structure of cooperatively breeding groups in Southern Lapwing Vanellus chilensis and test the prediction that offspring sex ratio is skewed towards the sex that helps. The social groups consisted of a breeding pair with one or two young (mostly males, 68%) from the previous breeding season, and offspring sex ratio was not skewed. Cooperative breeding in the Southern Lapwing is not the consequence of cooperative polygamy, but rather groups were formed by a mated pair and some of their offspring from previous nests as helpers. 相似文献
109.
The CTLH complex is a large, highly conserved eukaryotic complex composed of eight proteins that has been associated to several cellular functions, more often described as an E3 ubiquitin ligase complex involved in protein degradation through ubiquitination but also via vacuole-dependent degradation. A common feature observed in several components of this complex is the presence of the domains lissencephaly-1 homology (LisH) and C-terminal to LisH (CTLH). The LisH domain is found in several proteins involved in chromosome segregation, microtubule dynamics, and cell migration. Also, this domain participates in protein dimerization, besides affecting protein half-life, and influencing in specific cellular localization. Among the proteins found in the CTLH complex, Twa1 (Two-hybrid-associated protein 1 with RanBPM), also known as Gid8 (glucose-induced degradation protein 8 homolog) is the smallest, being a good model for structural studies by NMR. In this work we report the chemical shift assignments of the homodimeric LisH domain of Twa1, as a first step to determine its solution structure. 相似文献