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31.
The plasma membrane oxidase NtrbohD is responsible for AOS production in elicited tobacco cells 总被引:26,自引:0,他引:26
Simon-Plas F Elmayan T Blein JP 《The Plant journal : for cell and molecular biology》2002,31(2):137-147
A cDNA encoding a protein, NtrbohD, located on the plasma membrane and homologue to the flavocytochrome of the neutrophil NADPH oxidase, was cloned in tobacco. The corresponding mRNA was accumulated when tobacco leaves and cells were treated with the fungal elicitor cryptogein. After elicitation with cryptogein, tobacco cells transformed with antisense constructs of NtrbohD showed the same extracellular alkalinization as the control, but no longer produced active oxygen species (AOS). This work represents the first demonstration of the function of a homologue of gp91-phox in AOS production in elicited tobacco cells. 相似文献
32.
Toll L Khroyan TV Sonmez K Ozawa A Lindberg I McLaughlin JP Eans SO Shahien AA Kapusta DR 《FASEB journal》2012,26(2):947-954
Computational methods have led two groups to predict the endogenous presence of a highly conserved, amidated, 14-aa neuropeptide called either spexin or NPQ. NPQ/spexin is part of a larger prohormone that contains 3 sets of RR residues, suggesting that it could yield more than one bioactive peptide; however, no in vivo activity has been demonstrated for any peptide processed from this precursor. Here we demonstrate biological activity for two peptides present within proNPQ/spexin. NPQ/spexin (NWTPQAMLYLKGAQ-NH(2)) and NPQ 53-70 (FISDQSRRKDLSDRPLPE) have differing renal and cardiovascular effects when administered intracerebroventricularly or intravenously into rats. Intracerebroventricular injection of NPQ/spexin produced a 13 ± 2 mmHg increase in mean arterial pressure, a 38 ± 8 bpm decrease in heart rate, and a profound decrease in urine flow rate. Intracerebroventricular administration of NPQ 53-70 produced a 26 ± 9 bpm decrease in heart rate with no change in mean arterial pressure, and a marked increase in urine flow rate. Intraventricular NPQ/spexin and NPQ 53-70 also produced antinociceptive activity in the warm water tail withdrawal assay in mice (ED(50)<30 and 10 nmol for NPQ/spexin and NPQ 53-70, respectively). We conclude that newly identified peptides derived from the NPQ/spexin precursor contribute to CNS-mediated control of arterial blood pressure and salt and water balance and modulate nociceptive responses. 相似文献
33.
Adenot X Elmayan T Lauressergues D Boutet S Bouché N Gasciolli V Vaucheret H 《Current biology : CB》2006,16(9):927-932
34.
Alcione Silva de Carvalho Kelly Salom?o Solange Lisboa de Castro Taline Ramos Conde Helena Pereira da Silva Zamith Ernesto Raúl Caffarena Belinda Suzette Hall Shane Robert Wilkinson Núbia Boechat 《Memórias do Instituto Oswaldo Cruz》2014,109(3):315-323
Megazol (7) is a 5-nitroimidazole that is highly active against Trypanosoma
cruzi and Trypanosoma brucei, as well as drug-resistant
forms of trypanosomiasis. Compound 7 is not used clinically due to its mutagenic and
genotoxic properties, but has been largely used as a lead compound. Here, we compared
the activity of 7 with its 4H-1,2,4-triazole bioisostere (8) in
bloodstream forms of T. brucei and T. cruzi and
evaluated their activation by T. brucei type I nitroreductase
(TbNTR) enzyme. We also analysed the cytotoxic and genotoxic
effects of these compounds in whole human blood using Comet and fluorescein
diacetate/ethidium bromide assays. Although the only difference between 7 and 8 is
the substitution of sulphur (in the thiadiazole in 7) for nitrogen (in the triazole
in 8), the results indicated that 8 had poorer antiparasitic activity than 7 and was
not genotoxic, whereas 7 presented this effect. The determination of Vmax indicated
that although 8 was metabolised more rapidly than 7, it bounds to the
TbNTR with better affinity, resulting in equivalent kcat/KM
values. Docking assays of 7 and 8 performed within the active site of a homology
model of the TbNTR indicating that 8 had greater affinity than
7. 相似文献
35.