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61.
Junjie Miao Yongsheng Wang Chuanfei Guo Ye Tian Jianming Zhang Qian Liu Zhiping Zhou Hiroaki Misawa 《Plasmonics (Norwell, Mass.)》2012,7(2):377-381
In this paper, we study the nanoscale-focusing effect in the far field for a spiral plasmonic lens with a concentric annular groove by using finite-difference time domain simulation. The simulation result demonstrates that a left-hand spiral plasmonic lens can concentrate an incident right-hand circular polarization light into a focal spot at the exit surface. And this spot can be focused into far field due to constructive interference of the scattered light by the annular groove. The focal length and the focal depth can be adjusted by changing the groove radius and number of grooves within a certain range. These properties make it possible to probe the signal of spiral plasmonic lens in far field by using conventional optical devices. 相似文献
62.
Suzuki T Ueda A Kobayashi N Yang J Tomaru K Yamamoto M Takeno M Ishigatsubo Y 《The Biochemical journal》2008,411(3):581-591
ARMc8 (armadillo-repeat-containing protein 8) is a key component of the CTLH (C-terminal to lissencephaly type-1-like homology motif) complex in mammalian cells. This complex is well conserved in Saccharomyces cerevisiae and has been characterized as a FBPase (fructose-1, 6-bisphosphatase)-degrading complex. The yeast homologue of ARMc8, Gid (glucose-induced degradation) 5p, plays an essential role in the ubiquitin- and proteasome-dependent degradation of FBPase. To elucidate the function of ARMc8, we used a yeast two-hybrid system to screen a human skeletal muscle cDNA library. alpha-Catenin was isolated as a binding protein of ARMc8alpha. This association was confirmed by co-immunoprecipitation assay using MDCK (Madin-Darby canine kidney) cells in which exogenous alpha-catenin and ARMc8alpha were overexpressed. The association was also confirmed by co-immunoprecipitation assay using endogenous proteins in untransfected MDCK cells. We then used immunofluorescence microscopy of MDCK cells and C2C12 cells to investigate the intracellular distribution of ARMc8. Exogenously expressed ARMc8 was co-localized with alpha-catenin and beta-catenin along the cell membrane, suggesting an association between alpha-catenin and ARMc8 in the cells. To compare the binding domain of alpha-catenin with ARMc8alpha with that of beta-catenin, we performed a co-immunoprecipitation assay, again using 5'- and 3'-deletion constructs of alpha-catenin. The N-terminal sequence (amino acids 82-148) of alpha-catenin was sufficient to bind to both ARMc8alpha and beta-catenin. Next, we investigated the proteasome-dependent degradation of alpha-catenin by immunoblotting using proteasome inhibitors. Co-expression of ARMc8alpha with alpha-catenin resulted in rapid degradation of the exogenous alpha-catenin. Furthermore, ARMc8 knockdown inhibited alpha-catenin degradation and prolonged the half-life of alpha-catenin. We conclude that ARMc8alpha associates with alpha-catenin and up-regulates its degradation. 相似文献
63.
Nie X Nishitani C Yamazoe M Ariki S Takahashi M Shimizu T Mitsuzawa H Sawada K Smith K Crouch E Nagae H Takahashi H Kuroki Y 《Biochemistry》2008,47(48):12878-12885
Pulmonary surfactant protein D (SP-D) is a member of the collectin family and plays crucial roles in the innate immunity of the lung. We have previously shown that surfactant protein A (SP-A), a homologous collectin, interacts with MD-2 and alters lipopolysaccharide signaling. In this study, we examined and characterized the binding of SP-D to MD-2 using a soluble form of recombinant MD-2 (sMD-2). SP-D bound in a concentration- and Ca(2+)-dependent manner to sMD-2 coated onto microtiter wells. Excess mannose abolished the binding of SP-D to sMD-2. In solution, SP-D cosedimented with sMD-2 in the presence of Ca(2+). The direct binding of SP-D to sMD-2 was confirmed by BIAcore analysis. Anti-SP-D monoclonal antibody that recognizes the carbohydrate recognition domain (CRD) of SP-D significantly inhibited the binding of SP-D to sMD-2, indicating the involvement of the CRD for the binding to sMD-2. Ligand blot analysis revealed that SP-D bound to N-glycopeptidase F-treated sMD-2. In addition, the biotinylated SP-D pulled down the mutant sMD-2 with Asn(26) --> Ala and Asn(114) --> Ala substitutions, which lacks the consensus for N-glycosylation. Furthermore, the sMD-2 mutant cosedimented SP-D. These results demonstrate that SP-D directly interacts with MD-2 through the CRD. 相似文献
64.
Ryo Muramatsu Toru Negishi Tsutomu Mimoto Akira Miura Satoru Misawa Hideya Hayashi 《Journal of biotechnology》2002,93(2):131-142
A gene encoding for hirudin, a potent thrombin inhibitor, was expressed in Escherichia coli, which is the most widely used host. When the recombinant hirudin analog, CX-397, was overproduced by E. coli (600 mg l(-1)) in the absence of nutrient amino acids in the culture medium, the presence of two derivatives in the final product was observed with extremely increased retention times on reverse-phase high-performance liquid chromatography. Each derivative was due to methylation of an isoleucine residue at Ile29 or Ile59 in the CX-397. The structure was deducible as beta-methylnorleucine (beta MeNle; (2S,3S)-2-amino-3-methylhexanoic acid). The modification pathway of beta MeNle is not thought to be a post-translational modification of the protein because Ile has no functional group in its side-chain. Additionally, beta MeNle is synthesized by mutants of Serratia marcescens that belong to the same family, Enterobacteriaceae, as E. coli (J. Antibiot. 34 (1981a) 1278). These findings suggest that the lack of nutrient amino acids in the culture medium leads to the synthesis of beta MeNle in E. coli, which is then activated by E. coli isoleucyl-tRNA synthetase and incorporated into the overproduced recombinant protein. 相似文献
65.
I Kumagai M Inaizumi E Tamaki S Misawa T Hibino K Miura 《Nucleic acids symposium series》1988,(19):179-180
The high-level expression system of goat alpha-lactalbumin (alpha-LA) in E. coli was established by fusing the alpha-LA cDNA to porcine adenylate kinase cDNA and expressing the fused gene under the control of tac promoter. For high-level expression, elimination of 3'-noncoding region of the alpha-LA cDNA was found to be necessary. 相似文献
66.
An enzyme in Pseudomonas diminuta showed hydrolyzing activity of a benzhydrylamide ( = diphenylmethylamide) bond in S-benzylcysteinylglycine benzhydrylamide. The enzyme was purified 225-fold by precipitation with ammonium sulfate, and column chromatography with ECTEOLA-cellulose, DEAE-cellulose and hydroxyapatite. It showed an optimum pH of 6 to 8 and it was markedly inhibited by Hg2 + or p-chloromercuribenzoate. The preparation was more specific against S-benzylcysteinylglycine benzhydrylamide than other substrates tested. 相似文献
67.
Masanaru Misawa Hozumi Tanaka Osamu Chiyo Noboru Mukai 《Biotechnology and bioengineering》1975,17(3):305-314
A potent inhibitory agent against human plasmin, fibrinolytic proteinase, has been found in the extracts of callus tissue of Scopolia japonica. Effects of cultural conditions on cell growth and production of the plasmin inhibitory substance by this cell line in suspension cultures were examined in MurashigeSkoog's medium. More than l.5 mg of the inhibitor, as t-amino cyclohexane carboxylic acid, a synthetic plasmin inhibitor, were observed to accumulate per ml of medium containing 0.83 g of NH4NO3 and 7.6 g of KNO3 per liter as well as suitable levels of growth hormones. Addiction of antibiotics and deformers were examined in preliminary tests for large scale cultivation. Semicontinuous culture on a small scale in a glass cylinder, was also tested and growth rate of 1.29 g/liter/day (by dry wt) was obtained. Plasmin inhibitory activities in the extracts of the results intact plant and in cultured cells of S. japonica were compared and the results indicated that cell suspension culture was superior to extraction the natural plant for inhibitor production. 相似文献
68.
69.
Takashi Misawa Genichiro Tsuji Takeo Takahashi Eiji Ochiai Ken-ichiro Takagi Kyohei Horie Shinji Kakuda Midori Takimoto-Kamimura Masaaki Kurihara Yosuke Demizu 《Bioorganic & medicinal chemistry》2018,26(23-24):6146-6152
Non-secosteroidal VDR ligands without any assymmetric carbon were designed and synthesized based on the structure of the previously reported non-secosteroidal VDR agonist LG190178. The VDR-agonistic activity of all synthesized compounds was evaluated, and 7b emerged as a potent agonist activity with an EC50 value of 9.26?nM. Moreover, a docking simulation analysis was also performed to determine the binding mode of 7b with VDR-LBD. 相似文献
70.
Teiji Takechi Katsuhisa Koizumi Atsushi Azuma Masakazu Fukushima Katsutoshi Kobayashi Shinya Oda Katsuhiko Yanaga Leon Mullenders Peter Karran Masatsugu Ueda Yoshito Terai Minoru Ueki Masaru Sakamoto Aako Kondo Kiyohiko Miyake Yauko Koyamatsu Tsukasa Akiya Makoto Nakano Hiroshi Iwabuchi Tetsuya Muroya Yoshio Tenjin Kazunori Ochiai Tadao Tanaka Kyosuke Ymada Kazu Ueda Akihiko Misawa Aikou Okamoto Eizo Kimura Makoto Yasuda 《Human cell》2004,17(2):16-21